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PRJDA72415 DRP000446

Gut metagenomes screened for Kawasaki disease pathogens

Comprehensive Detection of Possible Pathogens Associated with Kawasaki Disease

80 gut metagenome samples (mostly neonates and infants) analyzed for possible pathogens associated with Kawasaki disease; country, design, and health status details not specified. machine-written summary

80 samples · 80 runs · Infant / toddler · 2012 · 0.74 Gbp / sample

Sources
✓Sequence archive 189–Abstract / description–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive189 sample values (age, health condition, health condition detail, subject id, timepoint)
– Abstract / descriptionno abstract or project description
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet · share metadata
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
Age
unknown50
neonate16
infant14
Condition
healthy_control10
Body site
primary62
excluded12
unknown6
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)this project, 0–0.03 Gbp: 2 samplesall projects, 0.03–0.1 Gbp: 17,066 samples (3 %)this project, 0.03–0.1 Gbp: 7 samplesall projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 10 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 28 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 19 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 14 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 0.74 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal

29 of 35 people sampled more than once (62 samples; median 2, up to 4 per person) · follow-up median 6 mo (max 6 mo) · median interval between samples 6 mo (time from age at collection). Schedule stated by the source: “Repeated sampling per subject across acute/distant phases and multiple days/months (day2-day6, 4month, 6month) based on sample_title tokens and timepoint_label diversity”.

P1P1: day 0P1: day 180P3P3: day 0P3: day 180P4P4: day 0P4: day 177P5P5: day 0P5: day 176P6P6: day 0P6: day 178P7P7: day 0P7: day 117P8P8: day 0P8: day 1180 d45 d3 mo4 mo6 mo

Each row is one person (7 shown of 29); dots are samples at days since that person's first sample.

How longitudinal and relatedness information is derived

Community composition
Bifidobacterium 24.8%Mediterraneibacter 7.9%Blautia_A 5.8%Bacteroides 5.5%Streptococcus 3.8%Escherichia 3.7%Ruminococcoides 3.3%Enterococcus_B 2.9%Phocaeicola 2.7%Enterococcus 2.5%Faecalibacterium 2.4%Fusicatenibacter 2.0%not assigned to a genus 11.6%other genera 21.2%

Genera, mean relative abundance over 51 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category30 38%
country0 0%
health_condition10 12%10
subject_id80 100%80
timepoint_label65 81%65
age_at_collection_days24 30%24
health_condition_detail10 12%10
Papers (1)
Details
How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of infant / toddler by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage verdict include”
  • body site external_curation.infant_catalog · “infant triage verdict include (gut/stool scope)”
  • life stage external_curation.infant_catalog · “infant triage verdict include (0-3 y scope)”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMD00011141age_at_collection_days4R10.85sample.titleday4
SAMD00011142health_conditionhealthy_controlR10.70sample.name_rule:TITLEC4-feces
SAMD00011142health_condition_detailC4-fecesR10.70sample.name_rule:TITLEC4-feces
SAMD00002748subject_idTYMC108R10.70sample.name_rule:TITLETYMC108-feces_Distant
SAMD00008983subject_idP1-026R10.70sample.name_rule:stP1-026A
SAMD00002748timepoint_labelDistantR10.70sample.name_rule:TITLETYMC108-feces_Distant
SAMD00008984timepoint_label026DR10.70sample.titleP1-026D
Sequencing

80 runs over 80 samples · mean 1.43 Gbp per run (median 0.74 Gbp, total 114.75 Gbp; 80 runs with a base count) · layout SINGLE 80 · platform ILLUMINA 80 · models Illumina Genome Analyzer II (80) · 75% of runs with a Sandpiper profile · first public 2012-10-22 – 2015-10-22

Authors (0)

No author record.

Help complete this project

3 of the 4 core fields are below 50% coverage: age_category, country, health_condition. Share metadata

Downloads

PRJDA72415.csv.gz (3.3 KB) · parquet (73.4 KB) — all 80 samples, every field with route and confidence · PRJDA72415_determinations.csv.gz (3.0 KB) — 189 evidence rows

Samples (80 · every field this project has values for · hover a cell for its source)
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