Fecal drying storage method evaluation, Japan
60 fecal samples from Japan were used to evaluate room-temperature storage by drying feces, assessing effects on microbiota, microbial pathways, and metabolome preservation. machine-written summary
60 samples · 60 runs · JP · Age unknown · 2023 · 4.77 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 240 sample values (collection date, country, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
longitudinal
10 of 10 people sampled more than once (60 samples; median 6, up to 6 per person) · follow-up median 14 d (max 14 d) · median interval between samples 7 d (time from timepoint numbers, unit from the stated sampling schedule). Schedule stated by the source: “Repeated sampling of same 10 subjects at day 0, 7, and 14 under different storage conditions (same biological sample split across methods/timepoints)”; stated follow-up 14 d.
Each row is one person (10 shown); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 52 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 0 | 0% | ||||
| country | 60 | 100% | 60 | |||
| health_condition | 0 | 0% | ||||
| subject_id | 60 | 100% | 60 | |||
| collection_date | 60 | 100% | 60 | |||
| timepoint_label | 60 | 100% | 60 |
Archive description
The objective was to evaluate the performance of storage at room temperature by drying feces for subsequent examination of the microbiota, microbial pathways, and the metabolome.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.60. Assay shotgun_dna.
- host run.scientific_name · “human gut metagenome”
- body site study.title · “fecal microbiota and metabolite preservation”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMD00601984 | collection_date | 2023-04-28 | R1 | 0.90 | biosample.attribute:collection_date | 2023-04-28 |
| SAMD00601984 | country | JP | R1 | 0.90 | sample.attr.geo_loc_name | Japan |
| SAMD00601984 | subject_id | 01 | R1 | 0.70 | sample.name_rule:sample_name | 01_LqN_0 |
| SAMD00601984 | timepoint_label | 0 | R1 | 0.70 | sample.name_rule:sample_name | 01_LqN_0 |
Sequencing
60 runs over 60 samples · mean 4.73 Gbp per run (median 4.77 Gbp, total 283.74 Gbp; 60 runs with a base count) · layout PAIRED 60 · platform ILLUMINA 60 · models HiSeq X Ten (60) · 87% of runs with a Sandpiper profile · first public 2023-06-05
Authors (0)
No author record.
Help complete this project
2 of the 4 core fields are below 50% coverage: age_category, health_condition. Share metadata
Downloads
PRJDB15798.csv.gz (2.2 KB) · parquet (70.8 KB) — all 60 samples, every field with route and confidence · PRJDB15798_determinations.csv.gz (2.1 KB) — 240 evidence rows