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PRJDB18359 DRP013446

Gut microbiota in dietary intervention study, Japan

Gut microbiota of dietary intervention study

45 gut microbiome samples from adults in Tokyo (Shinjuku) and Osaka (Settsu), Japan, collected as part of a dietary intervention study examining factors affecting energy balance via digestion and absorption rate. machine-written summary

45 samples · 45 runs · JP · Adult · 2025 · 7.75 Gbp / sample

Sources
✓Sequence archive 555○Abstract / description✓Full text 165○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive555 sample values (age, collection date, country, diet, diet detail, intervention, intervention detail, latitude)
○ Abstract / descriptionread; no usable statement
✓ Full text165 sample values (antibiotic exposure, diet, diet detail, health condition, health condition detail) · PMC12150606
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Interventions

intervention · arms resolved

dietdiet45 samples
single arm: samples on the intervention (no pre-intervention samples labelled)

Controlled / prescribed diet or feeding regimen design single_arm_before_after · Controlled diet study examining digestion/absorption and energy balance

“dietary intervention study UMIN000047018” — bioproject.description (study level; per-sample arms are in the intervention column below)

Samples at a glance
2 sampling locations · 1 country
Age
adult45
Condition
healthy_control45
Sex
male36
female9
Country
JP45
Body site
primary45
Collected 2022–2023
2022: 30 samples2023: 15 samples20222023
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 12 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 30 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 3 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 7.75 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal

15 of 15 people sampled more than once (45 samples; median 3, up to 3 per person) · follow-up median 2 mo (max 3 mo) · median interval between samples 31 d (time from collection months (month precision)). Schedule stated by the source: “feces collected during each 8-day condition (OF, CON, UF), days 5-8, crossover with ≥3 week washout” (time origin: intervention_start).

PN004449PN004449: day 0PN004449: day 31PN004449: day 92PN010127PN010127: day 0PN010127: day 28PN010127: day 59PN011471PN011471: day 0PN011471: day 31PN011471: day 61PN015289PN015289: day 0PN015289: day 28PN015289: day 59PN020633PN020633: day 0PN020633: day 31PN020633: day 61PN029461PN029461: day 0PN029461: day 31PN029461: day 61PN030917PN030917: day 0PN030917: day 61PN030917: day 92PN038158PN038158: day 0PN038158: day 28PN038158: day 59PN039673PN039673: day 0PN039673: day 31PN039673: day 61PN043923PN043923: day 0PN043923: day 30PN043923: day 61PN062288PN062288: day 0PN062288: day 59PN062288: day 89PN065449PN065449: day 0PN065449: day 61PN065449: day 92PN084226PN084226: day 0PN084226: day 0PN084226: day 31PN097445PN097445: day 0PN097445: day 0PN097445: day 31PN097497PN097497: day 0PN097497: day 30PN097497: day 610 d23 d46 d2 mo3 mo

Each row is one person (15 shown); dots are samples at days since that person's first sample.

How longitudinal and relatedness information is derived

Community composition
Bifidobacterium 14.7%Phocaeicola 14.0%Bacteroides 9.0%Prevotella 6.7%Faecalibacterium 6.2%Blautia_A 5.8%Megamonas 4.8%Lachnospira 2.6%Anaerostipes 2.4%Roseburia 2.4%Streptococcus 2.3%Parabacteroides 2.1%not assigned to a genus 3.1%other genera 23.9%

Genera, mean relative abundance over 45 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category45 100%
country45 100%45
health_condition45 100%45
subject_id45 100%45
age_at_collection_days45 100%45
antibiotic_exposure45 100%45
collection_date45 100%45
detailed_location45 100%
diet45 100%3015
diet_detail45 100%3015
health_condition_detail45 100%45
intervention45 100%45
intervention_detail45 100%45
latitude45 100%45
location_locality45 100%45
location_region45 100%45
longitude45 100%45
sex45 100%45
Papers (1)
  • Effect of short-term dietary intervention on fecal serotonin, gut microbiome-derived tryptophanase, and energy absorption in a randomized crossover trial: an exploratory analysis. 2025 PMID 40488306 PMC12150606 own_data
Details
Archive description

Shotgun metagenomic sequence of gut microbiota of dietary intervention study UMIN000047018: a study on the factors affecting the variation of energy balance focusing on the digestion and absorption rate.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.70. Assay shotgun_dna.

  • host run.scientific_name · “human gut metagenome”
  • body site study.title · “Gut microbiota of dietary intervention study”
  • life stage sample.attr.host_age · “20-24 (n=45/45)”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMD00797372age_at_collection_days7933.0R10.85sample.attr.host_age20-24
SAMD00797372antibiotic_exposurenoR30.70paper.body.methodsthe participants did not have digestion/absorption-related illnesses or use antibiotics
SAMD00797372collection_date2022-03R10.90biosample.attribute:collection_date2022-03
SAMD00797372countryJPR10.90sample.attr.geo_loc_nameJapan:Tokyo, Shinjuku
SAMD00797372diettherapeutic_or_study_dietR10.90biosample.attribute:host_dietOverfeeding
SAMD00797382diettherapeutic_or_study_dietR30.70paper.body.methodsAll diets had similar macronutrient profiles (15% protein, 30% fat, and 55% carbohydrate)
SAMD00797372diet_detailOverfeedingR10.90biosample.attribute:host_dietOverfeeding
SAMD00797382diet_detailcontrolled study diet varying only in energy intakeR30.70paper.body.methodsAll diets had similar macronutrient profiles (15% protein, 30% fat, and 55% carbohydrate)
SAMD00797372health_conditionhealthy_controlR30.70paper.body.methodsFifteen healthy males and females aged ≥20 years were included in this study
SAMD00797372health_condition_detailhealthy males and femalesR30.70paper.body.methodsFifteen healthy males and females aged ≥20 years were included in this study
SAMD00797372interventiondiet_interventionR10.90biosample.attribute:host_diethost_diet: Overfeeding
SAMD00797372intervention_detailoverfeeding (caloric excess) diet armR10.90biosample.attribute:host_diethost_diet: Overfeeding
SAMD00797372latitude35.6900R10.90biosample.attribute:lat_lon35.69 N 139.70 E
SAMD00797372location_localityShinjukuR10.85biosample.attribute:geo_loc_nameJapan:Tokyo, Shinjuku
SAMD00797372location_regionTokyoR10.85biosample.attribute:geo_loc_nameJapan:Tokyo, Shinjuku
SAMD00797372longitude139.7000R10.90biosample.attribute:lat_lon35.69 N 139.70 E
SAMD00797372sexmaleR10.90sample.attr.host_sexmale
SAMD00797372subject_idPN015289R10.85sample.attr.host_subject_idPN015289
Sequencing

45 runs over 45 samples · mean 6.45 Gbp per run (median 7.75 Gbp, total 290.37 Gbp; 45 runs with a base count) · layout PAIRED 45 · platform ILLUMINA 45 · models Illumina NovaSeq 6000 (45) · 100% of runs with a Sandpiper profile · first public 2025-06-18

Authors (0)

No author record.

Downloads

PRJDB18359.csv.gz (2.5 KB) · parquet (73.8 KB) — all 45 samples, every field with route and confidence · PRJDB18359_determinations.csv.gz (5.9 KB) — 720 evidence rows

Samples (45 · every field this project has values for · hover a cell for its source)
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