Gut microbiota in healthy young adults, cross-sectional
72 fecal metagenome samples from healthy individuals aged 20–25 years, analyzed by Kraken2 taxonomic classification after host-read removal; cross-sectional design. Country/location not reported (data agreement established pre-2023). machine-written summary
72 samples · 72 runs · Adult · 2025 · 14.08 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 360 sample values (age, bmi, collection date, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 216 sample values (health condition, health condition detail, location site) · PMC13404127 |
| ✓ Supplementary tables | 144 sample values (antibiotic exposure, sex) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
longitudinal
8 of 8 people sampled more than once (72 samples; median 9, up to 9 per person) · follow-up median 42 d (max 42 d) · median interval between samples 3 d (time from collection dates). Schedule stated by the source: “Participants self-collected fecal samples every 2-3 days (three samples per week) across ~40 days, two high-frequency sampling phases” (time origin: enrolment); stated follow-up 40 d.
Each row is one person (8 shown); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 68 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 72 | 100% | ||||
| country | 0 | 0% | ||||
| health_condition | 72 | 100% | 72 | |||
| subject_id | 72 | 100% | 72 | |||
| age_at_collection_days | 72 | 100% | 72 | |||
| antibiotic_exposure | 72 | 100% | 72 | |||
| bmi | 72 | 100% | 72 | |||
| collection_date | 72 | 100% | 72 | |||
| detailed_location | 72 | 100% | ||||
| health_condition_detail | 72 | 100% | 72 | |||
| location_site | 72 | 100% | 72 | |||
| sex | 72 | 100% | 72 | |||
| timepoint_label | 72 | 100% | 72 |
- Tracking Gut Homeostasis: Key Taxa Transitions and Core Network Hyper-Connectivity as Early Signals of Dysbiosis. 2026 PMID 42511982 PMC13404127 own_data
Archive description
This project encompasses metagenomic sequencing data derived from fecal samples of healthy individuals aged 20 - 25 years. The data underwent rigorous quality control. Post-quality control, the sequencing reads were aligned to the host genome to remove those that matched the host genome. Subsequently, taxonomic classification was performed using a customized Kraken2 microbial database.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.80. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site study.description · “fecal samples of healthy individuals”
- life stage study.description · “healthy individuals aged 20 - 25 years”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMD01622744 | age_at_collection_days | 9496.0 | R1 | 0.90 | sample.attr.host_age | 26 year |
| SAMD01622744 | antibiotic_exposure | no | R2 | 0.85 | paper.supp.biomedicines-14-01508-s001.zip/Supplementary Table S1.pdf[Antibiotics] | H2101A H2101 Female 26 20.83 A NO 2 |
| SAMD01622744 | bmi | 20.83 | R1 | 0.90 | sample.attr.host_body_mass_index | 20.83 |
| SAMD01622744 | collection_date | 2024-01-21 | R1 | 0.90 | biosample.attribute:collection_date | 2024-01-21 |
| SAMD01622744 | health_condition | healthy_control | R3 | 0.70 | paper.body.methods_results | 8 healthy subjects recruited from Central South University |
| SAMD01622744 | health_condition_detail | healthy subjects | R3 | 0.70 | paper.body.methods_results | 8 healthy subjects recruited from Central South University |
| SAMD01622744 | location_site | Central South University | R3 | 0.70 | paper.body.methods_results | recruited from Central South University |
| SAMD01622744 | sex | female | R2 | 0.85 | paper.supp.biomedicines-14-01508-s001.zip/Supplementary Table S1.pdf[Gender] | H2101A H2101 Female 26 20.83 A NO 2 |
| SAMD01622744 | subject_id | H2101 | R1 | 0.70 | sample.name_rule:title | H2101A (SAMC5113166) |
| SAMD01622744 | timepoint_label | A | R1 | 0.70 | sample.name_rule:title | H2101A (SAMC5113166) |
Sequencing
72 runs over 72 samples · mean 13.81 Gbp per run (median 14.08 Gbp, total 994.61 Gbp; 72 runs with a base count) · layout PAIRED 72 · platform ILLUMINA 72 · models Illumina NovaSeq 6000 (72) · 94% of runs with a Sandpiper profile · first public 2025-11-21
Authors (0)
No author record.
Downloads
PRJDB36826.csv.gz (2.9 KB) · parquet (73.8 KB) — all 72 samples, every field with route and confidence · PRJDB36826_determinations.csv.gz (6.3 KB) — 720 evidence rows