BL-99 probiotic trial for gut motility in older adults
110 gut metagenome samples from elderly participants in an intervention study testing the probiotic Bifidobacterium animalis subsp. lactis BL-99 for age-related intestinal hypomotility; sample location not reported. machine-written summary
110 samples · 110 runs · Adult · 2025 · 12.02 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 330 sample values (collection date, subject id, timepoint) |
| – Abstract / description | no abstract or project description |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Probiotic (live microorganisms; single strain or mixture) design randomized_controlled_trial · population other_disease · Bifidobacterium animalis subsp. lactis BL-99 supplementation vs placebo in older adults
“double-blind clinical trial and naturally aged mouse models, BL-99 intervention” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
55 of 55 people sampled more than once (110 samples; median 2, up to 2 per person). Schedule stated by the source: “Samples collected at two visit timepoints (V0, V1) in a double-blind clinical trial of BL-99 intervention” (time origin: intervention_start).
Genera, mean relative abundance over 104 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 110 | 100% | ||||
| country | 0 | 0% | ||||
| health_condition | 0 | 0% | ||||
| subject_id | 110 | 100% | 110 | |||
| collection_date | 110 | 100% | 110 | |||
| timepoint_label | 110 | 100% | 110 |
Archive description
Decay of smooth muscle contractility is one of major trigors of aging-related intestinal hypomotility, which imposes a substantial healthcare burden. Yet effective targeted therapy towards that is still unavailable. Here, we identified the probiotic Bifidobacterium animalis subsp. lactis BL-99 as a microbiota modulator that restores gut motility through bile acid (BA)-mediated rescue of smooth muscle contractile function. In a double-blind clinical trial and naturally aged mouse models, BL-99 intervention significantly reduced the whole-gut transit time and concurrently remodeled the gut microbiota. This suppressed bile salt hydrolase-expressing bacteria, which preserved conjugated BA pools, including taurochenodeoxycholic acid (TCDCA). Mechanistic investigations revealed that BL-99 activated the serum response factor(SRF)-myocardin (Myocd) transcriptional axis in colonic smooth muscle cells (SMCs), restoring contractile apparatus components (e.g., α-SMA, SM-MHC). Crucially, TCDCA administration recapitulated BL-99 regulation of intestinal SMC contractile protein expression by directly inhibiting the PI3K/AKT pathway. Our findings established a microbiota-BA-SMC signaling axis that ameliorates age-related intestinal hypomotility, positioning both BL-99 and TCDCA as precision therapeutic candidates targeting SMC-related dysmotility.
How this project entered the registry
Classified as human gut / stool / blood / tissue / internal fluids shotgun DNA metagenome of adult and elderly by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_unknown_no_evidence”
- body site study.title · “in promoting age-related intestinal peristalsis in older”
- body site study.description · “trigors of aging-related intestinal hypomotility, which imposes”
- body site study.description · “gut motility through bile acid (BA)-mediated rescue”
- life stage study.title · “peristalsis in older adults (PRJCA042472)”
- life stage study.title · “intestinal peristalsis in older adults (PRJCA042472)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMD01727886 | collection_date | 2023-11-22 | R1 | 0.90 | biosample.attribute:collection_date | 2023-11-22 |
| SAMD01727886 | subject_id | A-1 | R1 | 0.70 | sample.name_rule:title | V0-A-1 (SAMC5622037) |
| SAMD01727886 | timepoint_label | V0 | R1 | 0.70 | sample.name_rule:title | V0-A-1 (SAMC5622037) |
Sequencing
110 runs over 110 samples · mean 11.83 Gbp per run (median 12.02 Gbp, total 1,301.67 Gbp; 110 runs with a base count) · layout PAIRED 110 · platform ILLUMINA 110 · models HiSeq X Ten (110) · 95% of runs with a Sandpiper profile · first public 2025-12-21
Authors (0)
No author record.
Help complete this project
2 of the 4 core fields are below 50% coverage: country, health_condition. Share metadata
Downloads
PRJDB38865.csv.gz (3.3 KB) · parquet (73.0 KB) — all 110 samples, every field with route and confidence · PRJDB38865_determinations.csv.gz (3.8 KB) — 330 evidence rows