Gut microbiome and resistome in healthy Romanian adults
Cross-sectional study of 160 gut metagenome samples from a healthy Romanian cohort (158 adults, 2 elderly), characterizing microbiome composition, antimicrobial resistance genes, and correlations with cytokine-based immune responses. machine-written summary
160 samples · 160 runs · RO · Adult · 2026 · 8.42 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 1,440 sample values (age, collection date, country, health condition, health condition detail, latitude, location region, longitude) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 160 sample values (location site) · PMC13532179 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 160 | 100% | ||||
| country | 160 | 100% | 160 | |||
| health_condition | 160 | 100% | 160 | |||
| subject_id | 0 | 0% | ||||
| age_at_collection_days | 160 | 100% | 160 | |||
| collection_date | 160 | 100% | 160 | |||
| detailed_location | 160 | 100% | ||||
| health_condition_detail | 160 | 100% | 160 | |||
| latitude | 160 | 100% | 160 | |||
| location_region | 160 | 100% | 160 | |||
| location_site | 160 | 100% | 160 | |||
| longitude | 160 | 100% | 160 | |||
| sex | 160 | 100% | 160 |
- Resistome and microbiome-immune interactions in an Eastern European population with high antibiotic use. 2026 PMID 42578673 PMC13532179 own_data
Archive description
The gut microbiome influences host health, affecting gastrointestinal, metabolic, immune, cardiovascular, and neurological functions. A balanced microbiome is associated with favourable health outcomes. However, excessive antibiotic use and dietary habits can disrupt this ecosystem, leading to dysbiosis and affecting body homeostasis. This first comprehensive metagenomic analysis of the gut microbiome in a healthy Romanian cohort, a population underrepresented in microbiome studies and characterized by high antibiotic consumption, addresses a gap in current microbiome research. We report an enrichment of Enterobacteriaceae, although overall composition is more comparable to other European than non-European cohorts. Community configurations align with established enterotype patterns, and our analysis provides insight into their relationship with within-phylum diversity The analysis of antimicrobial resistance provides insight into the prevalence of resistance genes within this reservoir. We specifically report the presence of cfr(E), a Clostridioides difficile gene, and tet(X5), a variant from the ubiquitous tet family, genes not previously reported in healthy European populations. Integration with data from the European Centre for Disease Prevention and Control links the overall prevalence of resistance genes in this reservoir to antibiotic classes with higher community consumption in this population, notably beta-lactams and quinolones, highlighting potential targets for antibiotic stewardship programs. Finally, we investigate the relationship between the microbial profile and the systemic immune responses, inferred from correlations with in vitro cytokine production. Notably, we identify potential immune-priming roles for Collinsella, Flavonifractor and Bifidobacterium species.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.75. Assay shotgun_dna.
- host study.description · “gut microbiome in a healthy Romanian cohort”
- body site run.scientific_name · “human gut metagenome”
- life stage study.description · “healthy Romanian cohort”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA122737713 | age_at_collection_days | 14972.0 | R1 | 0.85 | sample.attr.host_age | 41.0 |
| SAMEA122737713 | collection_date | 2019-01-01 | R1 | 0.90 | biosample.attribute:collection_date | 2019-01-01 |
| SAMEA122737713 | country | RO | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Romania |
| SAMEA122737713 | health_condition | healthy_control | R1 | 0.85 | biosample.attribute:DESCRIPTION | healthy |
| SAMEA122737713 | health_condition_detail | healthy | R1 | 0.85 | biosample.attribute:DESCRIPTION | healthy |
| SAMEA122737713 | latitude | 44.3300 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 44.3300 23.7900 |
| SAMEA122737713 | location_region | Dolj | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 44.3300 23.7900 |
| SAMEA122737713 | location_site | Human Genomics Laboratory, University of Medicine and Pharmacy of Craiova | R3 | 0.70 | paper.fulltext.methods | at the Human Genomics Laboratory, University of Medicine and Pharmacy of Craiova |
| SAMEA122737713 | longitude | 23.7900 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 44.3300 23.7900 |
| SAMEA122737713 | sex | female | R1 | 0.90 | sample.attr.host_sex | female |
Sequencing
160 runs over 160 samples · mean 8.47 Gbp per run (median 8.42 Gbp, total 1,354.99 Gbp; 160 runs with a base count) · layout PAIRED 160 · platform DNBSEQ 160 · models DNBSEQ-G400 (160) · 0% of runs with a Sandpiper profile · first public 2026-06-01 – 2026-06-23
Authors (8)
Mirăuță B first · Riza AL · Streata I · Pirvu A · Dorobantu S · Dragos A · Surleac M · Netea MG last
Downloads
PRJEB113836.csv.gz (4.7 KB) · parquet (77.5 KB) — all 160 samples, every field with route and confidence · PRJEB113836_determinations.csv.gz (10.7 KB) — 1,600 evidence rows