Fungal nutraceutical trial in CRC surgery patients, Spain
81 fecal samples from adult colorectal cancer surgery patients in Spain, comparing Micodigest® fungal nutraceutical supplementation versus placebo in a randomized, double-blind, placebo-controlled intervention trial. machine-written summary
81 samples · 81 runs · ES · Adult · 2026 · 3.35 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 405 sample values (collection date, country, latitude, location region, longitude) |
| ✓ Abstract / description | 162 sample values (health condition, health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Non-microbial dietary supplement (vitamins design randomized_controlled_trial · population colorectal_cancer · Micodigest® fungal nutraceutical supplementation vs placebo, 4-6 weeks
“fungal nutraceutical supplementation with Micodigest® was associated with favorable” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 81 | 100% | ||||
| country | 81 | 100% | 81 | |||
| health_condition | 81 | 100% | 81 | |||
| subject_id | 0 | 0% | ||||
| collection_date | 81 | 100% | 81 | |||
| detailed_location | 81 | 100% | ||||
| health_condition_detail | 81 | 100% | 81 | |||
| latitude | 81 | 100% | 81 | |||
| location_region | 81 | 100% | 81 | |||
| longitude | 81 | 100% | 81 |
Archive description
The gut microbiome is increasingly recognized as an important determinant of host immune regulation, intestinal homeostasis, and recovery after colorectal cancer (CRC) surgery. Although our previous randomized, double-blind, placebo-controlled clinical trial demonstrated that fungal nutraceutical supplementation with Micodigest® was associated with favorable taxonomic and systemic inflammatory changes in patients undergoing CRC surgery with curative intent, the functional consequences of this intervention remain poorly understood. Here, we performed a functional metagenomic analysis of fecal samples collected from patients enrolled in the original clinical trial to investigate whether Micodigest® supplementation was associated with changes in the functional potential of the gut microbiome. A total of 37 patients were analyzed, including 24 receiving Micodigest® and 13 receiving placebo, with paired samples collected before supplementation and after 4–6 weeks of intervention. Shallow shotgun metagenomic sequencing was performed, and microbial functional potential was characterized using KEGG Orthologues (KOs). Differential abundance analysis identified 14 significantly different KOs between groups at baseline and 23 after intervention, with no overlap between the two sets of orthologues. Following supplementation, Micodigest®-treated patients exhibited increased representation of functions related to CRISPR-associated systems, bacteriophage biology, and chitin-derived carbohydrate utilization, together with reduced representation of nutrient acquisition systems, conjugative transfer, sulfur metabolism, and components of the Wood–Ljungdahl pathway. These functional signatures suggest coordinated changes in microbial ecological adaptation, nutrient utilization, genetic exchange, and metabolic activity following fungal nutraceutical supplementation. Integration with previously reported taxonomic and inflammatory findings supports the hypothesis that Micodigest® may promote coordinated remodeling of the gut microbiome and host–microbiota interactions during the perioperative period. Although the present study cannot establish causality or directly infer microbial activity from metagenomic potential, these findings provide novel functional insights into the microbiome response to fungal nutraceutical supplementation in patients with CRC and highlight functional metagenomics as a valuable complement to taxonomic profiling.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Potential of the Gut Microbiome in Colorectal”
- body site study.description · “The gut microbiome is increasingly”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA123357535 | collection_date | 2024-05-11 | R1 | 0.90 | biosample.attribute:collection_date | 2024-05-11 |
| SAMEA123357535 | country | ES | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Spain |
| SAMEA123357535 | health_condition | colorectal_cancer | R4 | 0.50 | study.description | patients undergoing CRC surgery with curative intent |
| SAMEA123357535 | health_condition_detail | colorectal cancer patients undergoing surgery | R4 | 0.50 | study.description | patients undergoing CRC surgery with curative intent |
| SAMEA123357535 | latitude | 42.2314 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 42.231358 -8.712447 |
| SAMEA123357535 | location_region | Galicia | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 42.231358 -8.712447 |
| SAMEA123357535 | longitude | -8.7124 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 42.231358 -8.712447 |
Sequencing
81 runs over 81 samples · mean 3.03 Gbp per run (median 3.35 Gbp, total 245.77 Gbp; 81 runs with a base count) · layout PAIRED 81 · platform ILLUMINA 81 · models NextSeq 1000 (81) · 0% of runs with a Sandpiper profile · first public 2026-08-21
Authors (0)
No author record.
Downloads
PRJEB123938.csv.gz (2.8 KB) · parquet (73.2 KB) — all 81 samples, every field with route and confidence · PRJEB123938_determinations.csv.gz (3.5 KB) — 567 evidence rows