Co-biotic formulations and ex vivo gut microbiome, Belgium

Dietary supplement formulations that contain co-biotics, or compounds that modulate biological processes in both the host and microbiome to confer a health benefit, are an emerging strategy to fine-tune both host physiology and gut microbiome function. Here, we evaluated three novel formulations containing co-biotic compounds (DM-02, a multivitamin AM-02, formulated for energy and focus and PM-02, formulated to promote sleep) for their effects on human gut microbiome composition and function. The three formulations and untreated control (Unt-Ctrl) were subjected to upper gastrointestinal (GI) digestion, after the digesta were exposed to 24 hour simulated colonic fermentation in the validated ex vivo SIFR technology (n=6 healthy adults). Outcomes included pH, short-chain fatty acid (SCFA) and gas production, ultra-deep metagenomic profiling, and untargeted metabolomics of post-colonic supernatants, each compared to Unt-Ctrl. All three formulations significantly reduced pH (3-4%) and increased total SCFAs (9-11%) and acetate (9-12%), with AM-02 and PM-02 also increasing butyrate by 20% and propionate by (7-8%), without increasing gas production. Each formulation significantly enriched specific SCFA- and B-vitamin-producing taxa. AM-02 significantly increased the abundance of two pyruvate fermentation to acetate/lactate pathways. PM-02 significantly increased the abundance of two tryptophan biosynthesis pathways, accompanied by an increase in available tryptophan and the abundance of tryptophan-producing microbes. All three formulations increased the availability of microbiome-derived metabolites, indicating microbiome functional modulation by the treatments. These findings support clinical evaluation of these novel formulations as a strategy to enhance microbiome composition and function.

Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.65. Assay shotgun_dna.

• host study.description · “human gut microbiome composition and function”
• body site study.description · “human gut microbiome composition and function”
• life stage study.description · “n=6 healthy adults”

30 runs over 30 samples · mean 13.90 Gbp per run (median 13.54 Gbp, total 417.10 Gbp; 30 runs with a base count) · layout PAIRED 30 · platform ELEMENT 30 · models Element AVITI (30) · 0% of runs with a Sandpiper profile · first public 2026-09-23

No author record.

PRJEB126105.csv.gz (1.7 KB) · parquet (72.0 KB) — all 30 samples, every field with route and confidence · PRJEB126105_determinations.csv.gz (3.5 KB) — 378 evidence rows