Gut microbiota in healthy adults after barley bread intervention
40 samples from healthy adults in a diet intervention study testing 3-day barley kernel-based bread consumption, comparing gut microbiota (Prevotella/Bacteroides ratio) between glucose-metabolism responders and non-responders. machine-written summary
40 samples · 40 runs · SE · Adult · 2026 · 2.41 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 280 sample values (collection date, country, latitude, location region, longitude, subject id, timepoint) |
| ✓ Abstract / description | 80 sample values (health condition, health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Controlled / prescribed diet or feeding regimen design single_arm_before_after · population healthy_control · 3-day barley kernel-based bread (BKB) feeding
“3-day consumption of barley kernel-based bread” — abstract:26552345 (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
20 of 20 people sampled more than once (40 samples; median 2, up to 2 per person). Schedule stated by the source: “Samples taken from healthy subjects before and after a 3-day consumption of barley kernel-based bread (BKB)” (time origin: intervention_start).
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 40 | 100% | ||||
| country | 40 | 100% | 40 | |||
| health_condition | 40 | 100% | 40 | |||
| subject_id | 40 | 100% | 40 | |||
| collection_date | 40 | 100% | 40 | |||
| detailed_location | 40 | 100% | ||||
| health_condition_detail | 40 | 100% | 40 | |||
| latitude | 40 | 100% | 40 | |||
| location_region | 40 | 100% | 40 | |||
| longitude | 40 | 100% | 40 | |||
| timepoint_label | 40 | 100% | 40 |
- Dietary Fiber-Induced Improvement in Glucose Metabolism Is Associated with Increased Abundance of Prevotella. 2015 PMID 26552345 own_data
Archive description
The gut microbiota plays an important role in human health by interacting with host diet, but there is substantial inter-individual variation in the response to diet. Here we compared the gut microbiota composition of healthy subjects who exhibited improved glucose metabolism following 3-day consumption of barley kernel-based bread (BKB) with those who responded least to this dietary intervention. The Prevotella/Bacteroides ratio was higher in responders than non-responders after BKB. Metagenomic analysis showed that the gut microbiota of responders was enriched in Prevotella copri and had increased potential to ferment complex polysaccharides after BKB. Finally, germ-free mice transplanted with microbiota from responder human donors exhibited improved glucose metabolism and increased abundance of Prevotella and liver glycogen content compared with germ-free mice that received non-responder microbiota. Our findings indicate that Prevotella plays a role in the BKB-induced improvement in glucose metabolism observed in certain individuals, potentially by promoting increased glycogen storage.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.75. Assay shotgun_dna.
- host study.description · “gut microbiota plays an important role in human health”
- body site study.description · “gut microbiota composition of healthy subjects”
- life stage study.description · “healthy subjects who exhibited improved glucose metabolism”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA123496358 | collection_date | 2012 | R1 | 0.90 | biosample.attribute:collection_date | 2012 |
| SAMEA123496358 | country | SE | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Sweden |
| SAMEA123496358 | health_condition | healthy_control | R4 | 0.40 | study.description | healthy subjects who exhibited improved glucose metabolism |
| SAMEA123496358 | health_condition_detail | All subjects described as healthy | R4 | 0.40 | study.description | healthy subjects who exhibited improved glucose metabolism |
| SAMEA123496358 | latitude | 55.7058 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 55.70584 13.19321 |
| SAMEA123496358 | location_region | Skane | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 55.70584 13.19321 |
| SAMEA123496358 | longitude | 13.1932 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 55.70584 13.19321 |
| SAMEA123496358 | subject_id | X01 | R1 | 0.70 | sample.name_rule:alias | X01-0 |
| SAMEA123496358 | timepoint_label | 0 | R1 | 0.70 | sample.name_rule:alias | X01-0 |
Sequencing
40 runs over 40 samples · mean 2.41 Gbp per run (median 2.41 Gbp, total 96.43 Gbp; 40 runs with a base count) · layout PAIRED 40 · platform ILLUMINA 40 · models Illumina HiSeq 4000 (40) · 0% of runs with a Sandpiper profile · first public 2026-09-23
Authors (10)
Kovatcheva-Datchary P first · Nilsson A · Akrami R · Lee YS · De Vadder F · Arora T · Hallen A · Martens E · Björck I · Bäckhed F last
Downloads
PRJEB127029.csv.gz (1.9 KB) · parquet (71.3 KB) — all 40 samples, every field with route and confidence · PRJEB127029_determinations.csv.gz (3.0 KB) — 360 evidence rows