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PRJEB21741 ERP024023

Gut DNA virome in healthy Wayampi adults, French Guiana

Metagenomic analysis of the DNA virome of faeces from healthy adult Wayampi Amerindians.

Cross-sectional study of 31 faecal samples from healthy adult Wayampi Amerindians in Trois-Sauts, French Guiana, analyzing DNA viral communities; eight donors were prior ESBL-producing Enterobacteria carriers. machine-written summary

31 samples · 31 runs · GF · Adult · 2018 · 0.19 Gbp / sample

Sources
✓Sequence archive 124✓Abstract / description 155–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive124 sample values (collection date, country, latitude, longitude)
✓ Abstract / description155 sample values (health condition, lifestyle, lifestyle detail, location locality, subject id)
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
adult31
Condition
healthy_control31
Country
GF31
Body site
primary31
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)this project, 0–0.03 Gbp: 2 samplesall projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 27 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 2 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 0.19 Gbp per sample0130Gbp
Sampling design and relatedness

community — not resolvedtribe — not resolved

31 adults sampled from one isolated Wayampi Amerindian community in Trois-Sauts, French Guiana.

How longitudinal and relatedness information is derived

Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category31 100%
country31 100%31
health_condition31 100%31
subject_id31 100%31
collection_date31 100%31
detailed_location31 100%
latitude31 100%31
lifestyle31 100%31
lifestyle_detail31 100%31
location_locality31 100%31
longitude31 100%31
Papers (1)
  • Isolation in small populations of Wayampi Amerindians promotes endemicity and homogenisation of their faecal virome, but its distribution is not entirely random. 2018 PMID 29272453 own_data
Details
Archive description

Samples were collected from 31 healthy adult Wayampi Amerindians from Trois-Sauts (French Guiana) in the 2010 campaign (October). Originaly, samples were collected and used to test for Enterobacteria producing extended spectrum beta-lactamases (E-ESBL) intestinal carriage.Samples consisted of faeces (n=31) from 31 different volunteers (18 females and 13 males).Eight individuals had been previously chatracterised as ESBL carrieres and the rest as noncarriers).Demographic, lifestyle, environment, and medical history data had been also collected.For all samples, the metagenomic analysis of the DNA viral communities was carried out, using the Illumina MiSeq with 300 bp x2 chemistry.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage reason_code age_unknown_no_evidence”
  • body site study.title · “DNA virome of faeces from healthy adult”
  • body site study.description · “spectrum beta-lactamases (E-ESBL) intestinal carriage.Samples consisted of”
  • life stage study.title · “faeces from healthy adult Wayampi Amerindians.”
  • life stage study.description · “from 31 healthy adult Wayampi Amerindians from”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMEA104161572collection_date2010R10.90biosample.attribute:collection_date2010
SAMEA104161572countryGFR10.90sample.attr.geographic_location_country_and_or_seaFrench Guiana
SAMEA104161572health_conditionhealthy_controlR40.50study.titlehealthy adult Wayampi Amerindians
SAMEA104161572latitude2.2450R10.90biosample.attribute:geographic_location_latitude+geographic_location_longitude2.245 -52.8772
SAMEA104161572lifestyleindigenous_communityR40.50paper.abstractisolated community of the Wayampi Amerindians
SAMEA104161572lifestyle_detailWayampi Amerindians, described as an isolated communityR40.50paper.abstractisolated community of the Wayampi Amerindians
SAMEA104161572location_localityTrois-SautsR40.45ena.study_descriptionfrom Trois-Sauts (French Guiana)
SAMEA104161572longitude-52.8772R10.90biosample.attribute:geographic_location_latitude+geographic_location_longitude2.245 -52.8772
SAMEA104161572subject_idSAMEA104161572R40.65design.one_sample_per_subject:descriptionSamples consisted of faeces (n=31) from 31 different volunteers (18 females and 13 males)
Sequencing

31 runs over 31 samples · mean 0.19 Gbp per run (median 0.19 Gbp, total 5.98 Gbp; 31 runs with a base count) · layout PAIRED 31 · platform ILLUMINA 31 · models Illumina MiSeq (31) · 0% of runs with a Sandpiper profile · first public 2018-01-11

Authors (0)

No author record.

Downloads

PRJEB21741.csv.gz (1.8 KB) · parquet (71.6 KB) — all 31 samples, every field with route and confidence · PRJEB21741_determinations.csv.gz (2.4 KB) — 279 evidence rows

Samples (31 · every field this project has values for · hover a cell for its source)
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