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PRJEB26089 ERP108062

Vegetarian diet impact on gut microbiota, healthy adults, Sweden

Impact-of-Vegetarian-Diet

42 samples from healthy adult volunteers at Karolinska Institutet, Sweden, studying the effect of short- and long-term lacto-ovo-vegetarian diet on gut microbiota and immune repertoire, as a diet intervention study. machine-written summary

42 samples · 42 runs · SE · Age unknown · 2018 · 5.50 Gbp / sample

Sources
✓Sequence archive 252✓Abstract / description 42✓Full text 84○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive252 sample values (collection date, country, latitude, location region, location site, longitude)
✓ Abstract / description42 sample values (health condition detail)
✓ Full text84 sample values (antibiotic exposure, health condition) · PMC5934425
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Interventions

intervention — arms not resolved

Controlled / prescribed diet or feeding regimen design single_arm_before_after · population healthy_control · 3-months lacto-ovo-vegetarian diet in healthy omnivorous volunteers

“3-months lacto-ovo-vegetarian diet on the diversity” — abstract:29755475 (study level; per-sample arms are in the intervention column below)

Samples at a glance
1 sampling locations · 1 country
Age
adult42
Condition
healthy_control42
Country
SE42
Body site
primary42
Collected 2013–2015
2013: 23 samples2015: 19 samples20132015
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 42 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 5.50 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

Schedule stated by the source: “Samples collected from all participants at day 0 and day 91 (before and after 3-month diet period)” (time origin: start of diet change (study enrollment)); stated follow-up 3 mo.

How longitudinal and relatedness information is derived

Community composition
Bacteroides 9.9%Faecalibacterium 7.9%Prevotella 7.1%Bifidobacterium 6.6%Phocaeicola 6.0%Alistipes 4.1%Roseburia 2.8%Vescimonas 2.7%Dialister 1.9%Ligilactobacillus 1.5%Collinsella 1.5%Hominicoprocola 1.4%not assigned to a genus 4.8%other genera 41.8%

Genera, mean relative abundance over 35 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category42 100%
country42 100%42
health_condition42 100%42
subject_id0 0%
antibiotic_exposure42 100%42
collection_date42 100%42
detailed_location42 100%
health_condition_detail42 100%42
latitude42 100%42
location_region42 100%42
location_site42 100%42
longitude42 100%42
Papers (1)
  • Impact of a 3-Months Vegetarian Diet on the Gut Microbiota and Immune Repertoire. 2018 PMID 29755475 PMC5934425 own_data
Details
Archive description

Impact of short- and long-term vegetarian diet on the gut microbiota and immune repertoire

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by opus_adjudicated with confidence 0.75. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • host run.scientific_name · “human gut metagenome”
  • body site study.description · “vegetarian diet on the gut microbiota”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMEA4585769antibiotic_exposurenoR30.70paper.fulltext.methodshad not taken antibiotics 3 months before the study
SAMEA4585769collection_date2015-04-28R10.90biosample.attribute:collection_date2015-04-28
SAMEA4585769countrySER10.90sample.attr.geo_loc_nameSweden:Karolinska Institutet
SAMEA4585769health_conditionhealthy_controlR30.60paper.fulltext.resultsTwenty-nine healthy volunteers were enrolled as three groups
SAMEA4585769health_condition_detailhealthy volunteers in a vegetarian diet intervention trialR40.50paper.abstracthealthy omnivorous volunteers
SAMEA4585769latitude59.3500R10.90biosample.attribute:lat_lon59.35 N,18.03 E
SAMEA4585769location_regionStockholmR10.60biosample.attribute:lat_lon59.35 N,18.03 E
SAMEA4585769location_siteKarolinska InstitutetR10.85biosample.attribute:geo_loc_nameSweden:Karolinska Institutet
SAMEA4585769longitude18.0300R10.90biosample.attribute:lat_lon59.35 N,18.03 E
Sequencing

42 runs over 42 samples · mean 5.52 Gbp per run (median 5.50 Gbp, total 231.76 Gbp; 42 runs with a base count) · layout PAIRED 42 · platform ILLUMINA 42 · models Illumina HiSeq 2000 (42) · 83% of runs with a Sandpiper profile · first public 2018-06-29 – 2018-07-01

Authors (13)

Zhang C first · Björkman A · Cai K · Liu G · Wang C · Li Y · Xia H · Sun L · Kristiansen K · Wang J · Han J · Hammarström L · Pan-Hammarström Q last

Downloads

PRJEB26089.csv.gz (2.0 KB) · parquet (71.8 KB) — all 42 samples, every field with route and confidence · PRJEB26089_determinations.csv.gz (3.1 KB) — 378 evidence rows

Samples (42 · every field this project has values for · hover a cell for its source)
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