Gut microbiome in children given iron supplement IHAT trial
1605 gut metagenome samples from young children (ages unknown/neonate) in a randomized trial assessing dietary supplementation with the nano-iron compound IHAT for iron deficiency/anemia, sampled at three time points. machine-written summary
1,605 samples · 1,701 runs · GM · Infant / toddler · 2019 · 57.2 Mbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 3,150 sample values (subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 10,220 sample values (age, antibiotic exposure, country, lifestyle, lifestyle detail, location locality, location region) · PMC12701053, PMC8727306 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Non-microbial dietary supplement (vitamins design randomized_controlled_trial · population malnutrition · IHAT nano iron compound supplementation vs ferrous sulphate in children
“following IHAT administration” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
Schedule stated by the source: “Three time points following IHAT administration; sample titles show day codes D1, D15, D85.” (time origin: intervention start); stated follow-up 3 mo.
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 590 | 37% | ||||
| country | 1,605 | 100% | 1,605 | |||
| health_condition | 0 | 0% | ||||
| subject_id | 1,605 | 100% | 1,605 | |||
| antibiotic_exposure | 1,605 | 100% | 1,605 | |||
| detailed_location | 1,605 | 100% | ||||
| lifestyle | 1,605 | 100% | 1,605 | |||
| lifestyle_detail | 1,605 | 100% | 1,605 | |||
| location_locality | 1,605 | 100% | 1,605 | |||
| location_region | 1,605 | 100% | 1,605 | |||
| timepoint_label | 1,545 | 96% | 1,545 | |||
| age_at_collection_days | 590 | 37% | 590 |
- Gut microbiomes from Gambian infants reveal the development of a non-industrialized Prevotella-based trophic network. PMID 34972822 PMC8727306 curated
- Gut Prevotella stercorea associates with protection against infection in rural African children. PMID 41387413 PMC12701053 curated
Archive description
The aim of the project is to demonstrate for the first time that a novel and unique nano iron compound, which is an engineered analogue of natural food iron, SAFELY corrects iron deficiency and anemia in children in resource-poor areas. Our award-winning compound (Iron Hydroxide Adipate Tartrate: IHAT) is highly bioavailable to humans through physiologically appropriate pathways and is not available to gut enteropathogens. Importantly, IHAT has a low cost of manufacture due to its facile synthesis and inexpensive raw materials. It therefore has the potential to transform treatment of iron deficiency. Iron deficiency (ID) and its associated anemia (IDA) is the largest nutritional deficiency disorder in the world and affects a total of 1.2 billion people, the majority of whom are children and women from resource-poor countries. ID is frequently exacerbated by concomitant parasitic and bacterial enteropathogenic infections and contributes to almost 0.8 million deaths a year as well as irreparably limiting the cognitive development of children (1). In this study we will analyse the gut microbiome in young children over three different time points following IHAT administration. The effect of IHAT therpay and the changes of the gut microiobme will be investigated. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of infant / toddler and child and adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage verdict include”
- body site study.description · “not available to gut enteropathogens. Importantly, IHAT”
- body site external_curation.infant_catalog · “infant triage verdict include (gut/stool scope)”
- life stage external_curation.infant_catalog · “infant triage verdict include (0-3 y scope)”
- life stage study.description · “and anemia in children in resource-poor areas.”
- life stage study.description · “are children and women from resource-poor countries.”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA5080321 | age_at_collection_days | 1 | R3 | 0.80 | paper.fulltext.methods | collected at baseline (day 1) |
| SAMEA5080321 | antibiotic_exposure | no | R3 | 0.75 | paper.fulltext.methods | children who received antibiotics were also removed |
| SAMEA5080321 | country | GM | R3 | 0.40 | paper.fulltext.methods | living in the north bank rural communities |
| SAMEA5080321 | lifestyle | rural_non_industrialized | R3 | 0.70 | paper.fulltext.methods | living in the north bank rural communities in the Upper River |
| SAMEA5080321 | lifestyle_detail | Rural Gambian children, Upper River Region | R3 | 0.60 | paper.fulltext.methods | living in the north bank rural communities in the Upper River |
| SAMEA5080321 | location_locality | Wuli and Sandu districts | R3 | 0.70 | paper.fulltext.methods | study area included 45 villages in the Wuli and Sandu districts |
| SAMEA5080321 | location_region | Upper River Region | R3 | 0.70 | paper.fulltext.methods | in the Upper River Region (URR) of The Gambia |
| SAMEA5080321 | subject_id | IHAT_GUT7660077 | R1 | 0.85 | biosample_attr | IHAT_GUT7660077 |
| SAMEA5080321 | timepoint_label | D1 | R1 | 0.70 | sample.title | IHAT_004F_D1 |
Sequencing
1,701 runs over 1,605 samples · mean 64.9 Mbp per run (median 56.8 Mbp, total 110.46 Gbp; 1,701 runs with a base count) · layout PAIRED 1,701 · platform ILLUMINA 1,701 · models Illumina MiSeq (1,701) · 0% of runs with a Sandpiper profile · first public 2019-12-03 – 2020-03-03
Authors (11)
de Goffau MC first · Jallow AT · Sanyang C · Prentice AM · Meagher N · Price DJ · Revill PA · Parkhill J · Pereira DIA · Wagner J last · Ofordile O first
Help complete this project
2 of the 4 core fields are below 50% coverage: age_category, health_condition. Share metadata
Downloads
PRJEB28671.csv.gz (41.0 KB) · parquet (145.7 KB) — all 1,605 samples, every field with route and confidence · PRJEB28671_determinations.csv.gz (80.6 KB) — 13,370 evidence rows