Gut virome in adult monozygotic twins, cross-sectional
91 gut virome samples from adult monozygotic twin pairs selected for high or low microbiome concordance, examining the relationship between microbiome and virome diversity in a cross-sectional design. machine-written summary
91 samples · 92 runs · US · Adult · 2019 · 0.54 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 273 sample values (family id, family role, subject id) |
| ✓ Abstract / description | 91 sample values (health condition detail) |
| ✓ Full text | 91 sample values (country) · PMC11986028, PMC6411085, PMC7062461 |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
longitudinal — not resolvedtwins
45 of 46 people sampled more than once (90 samples; median 2, up to 2 per person).
21 adult monozygotic twin pairs + 2 additional twin pairs (A/B co-twins); co-twins not cohabiting · 23 families with 46 sampled people, 46 twin sets.
twin · circles are people (pale = no sequenced sample), arrows run donor → recipient and parent → child, curved lines join twins, partners and siblings, squares are shared settings or families without recorded roles. Shown: 23 linked groups from explicit relations. 46 people in the project overall.
Families (23)
Genera, mean relative abundance over 45 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 91 | 100% | ||||
| country | 91 | 100% | 91 | |||
| health_condition | 0 | 0% | ||||
| subject_id | 91 | 100% | 91 | |||
| family_id | 91 | 100% | 91 | |||
| family_role | 91 | 100% | 91 | |||
| health_condition_detail | 91 | 100% | 91 |
- Virome Diversity Correlates with Intestinal Microbiome Diversity in Adult Monozygotic Twins. 2019 PMID 30763537 PMC6411085 own_data
- Resurrection of a global, metagenomically defined gokushovirus. 2020 PMID 32101162 PMC7062461 unsure
- Characterization of the phyllosphere virome of fresh vegetables and potential transfer to the human gut. 2025 PMID 40210629 PMC11986028 unsure
Archive description
The human gut virome varies far more between hosts than the microbiome. Within twin-pairs, viromes have been shown to be similar for infants but not for adults, indicating that as twins age and their environments diverge, so do their viromes. The degree to which the microbiome drives the virome’s vast diversity is unclear. Here, we examined the relationship between microbiome diversity and virome diversity in 21 adult monozygotic twin pairs selected for high or low microbiome concordance. Viromes derived from virus-like particles were unique to each subject, dominated by Caudovirales and Microviridae, and exhibited a small core that included crAssphage. Microbiome-discordant twins had more dissimilar viromes compared to microbiome-concordant twins, and the richer the microbiomes, the richer the viromes. These patterns were driven by the bacteriophages, not eukaryotic viruses. These observations support a strong role of the microbiome in patterning the virome.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.72. Assay shotgun_dna.
- host study.title · “adult monozygotic twins with concordant”
- body site study.title · “discordant gut microbiomes”
- life stage study.title · “adult monozygotic twins”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA5065314 | country | US | R3 | 0.70 | paper.fulltext.methods | approved by the Cornell University IRB |
| SAMEA5065314 | family_id | 3 | R1 | 0.70 | sample.name_rule:TITLE | TITLE: 3B |
| SAMEA5065314 | family_role | twin | R1 | 0.70 | sample.name_rule:TITLE | TITLE: 3B |
| SAMEA5065314 | health_condition_detail | mixed_case_control: Twin pairs selected for microbiome concordance status; no explicit health/disease status stated for | R4 | 0.45 | study.description | selected for high or low microbiome concordance |
| SAMEA5065314 | subject_id | 3B | R1 | 0.70 | sample.name_rule:title | 3B |
Sequencing
92 runs over 91 samples · mean 0.62 Gbp per run (median 0.54 Gbp, total 57.19 Gbp; 92 runs with a base count) · layout PAIRED 84, SINGLE 8 · platform ILLUMINA 92 · models Illumina HiSeq 2500 (92) · 86% of runs with a Sandpiper profile · first public 2019-02-08 – 2020-07-29
Authors (19)
Moreno-Gallego JL first · Chou SP · Di Rienzi SC · Goodrich JK · Spector TD · Bell JT · Youngblut ND · Hewson I · Reyes A · Ley RE last · Kirchberger PC first · Ochman H last · Park JW first · Yun YE · Cho JA · Yoon SI · In SA · Park EJ · Kim MS last
Downloads
PRJEB29491.csv.gz (3.1 KB) · parquet (73.2 KB) — all 91 samples, every field with route and confidence · PRJEB29491_determinations.csv.gz (5.2 KB) — 455 evidence rows