Gut microbiome & vaccine response in middle-aged vs elderly
74 faecal samples from a study comparing DTP and influenza vaccine responses between middle-aged (30–50) and older (>70) participants, collected before vaccination and six weeks after. machine-written summary
74 samples · 148 runs · Elderly · 2020 · 6.00 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 74 sample values (subject id) |
| – Abstract / description | no abstract or project description |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Vaccination as the study intervention design single_arm_before_after · population healthy_control · DTP and influenza vaccination, sampled pre- and 6-weeks post-vaccination
“effects of DTP and influenza vaccination on the immune systems” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
Schedule stated by the source: “Faecal samples collected prior to vaccination and 6 weeks post vaccination” (time origin: intervention_start); stated follow-up 42 d.
Genera, mean relative abundance over 73 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 0 | 0% | ||||
| country | 0 | 0% | ||||
| health_condition | 0 | 0% | ||||
| subject_id | 74 | 100% | 74 |
Archive description
The world’s population is aging, with dramatic rises predicted in the coming decades, thus a healthy aged population is a major public health priority. However, the immune system declines with increasing age leading to increased susceptibility to infectious diseases, and suboptimal responses to vaccines, although this area remains poorly researched and the mechanisms are not understood. This project investigates the effects of DTP and influenza vaccination on the immune systems and compares the immune responses and vaccine efficacy between middle aged (30 – 50) and older (>70) participants. This project has applied state-of-the-art immunological methods to investigate the immune response to these two vaccines to determine how they modulate the immune system in the elderly and how they interact. In addition to immunological measurements, 64 faecal samples have been collected prior to vaccine delivery and 6-weeks post vaccination. Sample have been stored and DNA extracted according to the standardised processes and will enable investigation of the microbiome. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult and elderly by opus_adjudicated with confidence 0.75. Assay shotgun_dna.
- host study.description · “middle aged (30 – 50) and older (>70) participants”
- body site study.description · “64 faecal samples have been collected”
- life stage study.description · “middle aged (30 – 50) and older (>70) participants”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA5698114 | subject_id | VITAL7987993 | R1 | 0.85 | sample.attr.subject_id | VITAL7987993 |
Sequencing
148 runs over 74 samples · mean 3.03 Gbp per run (median 3.00 Gbp, total 448.01 Gbp; 148 runs with a base count) · layout PAIRED 148 · platform ILLUMINA 148 · models HiSeq X Ten (74), Illumina HiSeq 4000 (74) · 91% of runs with a Sandpiper profile · first public 2020-06-30 – 2021-01-26
Authors (0)
No author record.
Help complete this project
3 of the 4 core fields are below 50% coverage: age_category, country, health_condition. Share metadata
Downloads
PRJEB32834.csv.gz (2.7 KB) · parquet (71.9 KB) — all 74 samples, every field with route and confidence · PRJEB32834_determinations.csv.gz (1021 B) — 74 evidence rows