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PRJEB37898 ERP121241

Gut microbiota and MDRO colonisation in nursing home residents

MDROs and gut microbiota in a nursing home

12 faecal samples from elderly residents of a nursing home in the Netherlands, studied longitudinally for colonisation with multidrug-resistant organisms and gut microbiota composition. machine-written summary

12 samples · 12 runs · NL · Elderly · 2020 · 0.75 Gbp / sample

Sources
✓Sequence archive 68○Abstract / description✓Full text 24○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive68 sample values (collection date, country, latitude, longitude, subject id, timepoint)
○ Abstract / descriptionread; no usable statement
✓ Full text24 sample values (lifestyle, lifestyle detail) · PMC8028076
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
elderly12
Country
NL12
Body site
primary12
Collected 2016–2020
2016: 5 samples2017: 5 samples2019: 1 samples2020: 1 samples20162020
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 11 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 1 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 0.75 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinalcare home — not resolved

5 of 5 people sampled more than once (10 samples; median 2, up to 2 per person) · follow-up median 4 mo (max 4 mo) · median interval between samples 4 mo (time from collection dates). Schedule stated by the source: “four time points at 2-month intervals (October 2016-April 2017)” (time origin: enrolment); stated follow-up 6 mo.

L001L001: day 0L001: day 105L006L006: day 0L006: day 111L028L028: day 0L028: day 108L031L031: day 0L031: day 110R003R003: day 0R003: day 1110 d28 d56 d3 mo4 mo

Each row is one person (5 shown); dots are samples at days since that person's first sample.

5 residents of one Dutch nursing home (8 wards; ward per resident not given), 2 samples each.

How longitudinal and relatedness information is derived

Community composition
Bifidobacterium 26.9%Bacteroides 8.0%Methanocatella 6.4%Alistipes 4.7%Longicatena 4.6%Phocaeicola 3.9%Scatacola_A 3.6%Mediterraneibacter 3.5%Vescimonas 2.3%Fimivicinus 2.0%Frisingicoccus 1.7%Faecalibacterium 1.6%not assigned to a genus 9.6%other genera 21.1%

Genera, mean relative abundance over 1 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category12 100%
country12 100%12
health_condition0 0%
subject_id10 83%10
collection_date12 100%12
latitude12 100%12
lifestyle12 100%12
lifestyle_detail12 100%12
longitude12 100%12
timepoint_label10 83%10
Papers (1)
  • Microbiota-associated risk factors for asymptomatic gut colonisation with multi-drug-resistant organisms in a Dutch nursing home. 2021 PMID 33827686 PMC8028076 own_data
Details
Archive description

To be included

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of elderly by deterministic_prior with confidence 0.90. Assay shotgun_dna.

  • host external_curation.infant_catalog · “infant triage reason_code age_unknown_no_evidence”
  • body site study.title · “MDROs and gut microbiota in a”
  • life stage study.title · “microbiota in a nursing home”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMEA6796758collection_date2016-12-20R10.90biosample.attribute:collection_date2016-12-20
SAMEA6796758countryNLR10.90sample.attr.geographic_location_country_and_or_seaNetherlands
SAMEA6796758latitude52.0000R10.90biosample.attribute:geographic_location_latitude+geographic_location_longitude52 4,4931
SAMEA6796758lifestyleinstitutionalizedR30.70paper.fulltext.methodsresidents of a nursing home in the Netherlands
SAMEA6796758lifestyle_detailnursing home residentsR30.70paper.fulltext.methodsresidents of a nursing home in the Netherlands
SAMEA6796758longitude4.4931R10.90biosample.attribute:geographic_location_latitude+geographic_location_longitude52 4,4931
SAMEA6796758subject_idL001R10.70sample.name_rule:attr.descriptionFeces at time point 2 L001
SAMEA6796758timepoint_label2R10.70sample.name_rule:attr.descriptionFeces at time point 2 L001
Sequencing

12 runs over 12 samples · mean 0.74 Gbp per run (median 0.75 Gbp, total 8.92 Gbp; 12 runs with a base count) · layout PAIRED 12 · platform ILLUMINA 12 · models Illumina NovaSeq 6000 (12) · 8% of runs with a Sandpiper profile · first public 2020-07-14

Authors (11)

Ducarmon QR first · Terveer EM · Nooij S · Bloem MN · Vendrik KEW · Caljouw MAA · Sanders IMJG · van Dorp SM · Wong MC · Zwittink RD · Kuijper EJ last

Downloads

PRJEB37898.csv.gz (1.3 KB) · parquet (70.5 KB) — all 12 samples, every field with route and confidence · PRJEB37898_determinations.csv.gz (1.3 KB) — 92 evidence rows

Samples (12 · every field this project has values for · hover a cell for its source)
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