Gut microbiota across infants, adults, elderly, Spain
304 longitudinal fecal samples from healthy infants, adults, and elderly volunteers in the Valencian Community, Spain, analyzed by 16S rRNA, metagenomics, and metatranscriptomics to study core microbiota and age-related host-microbe decoupling. machine-written summary
304 samples · 304 runs · ES · Child · 2023 · 0.19 Gbp / sample · cohort Lifetime existence of a core of mutualistic symbio
One study deposited as 2 BioProjects (363 catalog samples together): PRJEB39661 · PRJEB62571 · show together
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 2,736 sample values (collection date, country, health condition, health condition detail, latitude, location region, longitude, subject id) |
| ○ Abstract / description | read; no usable statement |
| ○ Full text | read; no usable statement or table · PMC12873169 |
| ✓ Supplementary tables | 742 sample values (age, lifestyle, sex) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
longitudinal — not resolved
30 of 30 people sampled more than once (304 samples; median 11, up to 11 per person). Schedule stated by the source: “Eight fecal samples collected from each volunteer separated by a month”.
Genera, mean relative abundance over 4 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 134 | 44% | ||||
| country | 304 | 100% | 304 | |||
| health_condition | 304 | 100% | 304 | |||
| subject_id | 304 | 100% | 304 | |||
| collection_date | 304 | 100% | 304 | |||
| detailed_location | 304 | 100% | ||||
| health_condition_detail | 304 | 100% | 304 | |||
| latitude | 304 | 100% | 304 | |||
| lifestyle | 304 | 100% | 304 | |||
| location_region | 304 | 100% | 304 | |||
| longitude | 304 | 100% | 304 | |||
| sex | 304 | 100% | 304 | |||
| timepoint_label | 304 | 100% | 304 | |||
| age_at_collection_days | 134 | 44% | 134 |
- Lifetime existence of a core of mutualistic symbionts and functionally uncoupled taxa in the gut of a Mediterranean cohort. 2026 PMID 41513932 PMC12873169 unsure
Archive description
With the present work we intend to demonstrate: 1) the existence of a core of microbial taxa that are true mutualistic symbionts andare present throughout the life of the host and, 2) the existence of microbial taxa that are not responding to host demands, exposing a decoupling between these taxa and the human host. For that, thirty healthy volunteers from Valencian Community (Spain) were involved in this study. The cohort IAE was stablished with 10 Infants (I) 2-5 years old, 10 Adults (A), 25-45 years old, and 10 elderly (E) 65-85 years old. Eight fecal samples were collected from each volunteer separated by a month. For all samples, the metagenomic, 16S rRNA and metatranscriptomic analysis of the microbiota was carried out by Illumina NextSeq with 150 bp x2 chemistry (metagenomic and metareanscriptomic) or by Illumina Miseq (16S).
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of child and infant / toddler and adult and elderly by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_child_over_36m”
- body site study.description · “years old. Eight fecal samples were collected”
- body site external_curation.infant_catalog · “body_site_call feces”
- life stage external_curation.infant_catalog · “infant triage reason_code age_child_over_36m”
- life stage study.description · “stablished with 10 Infants (I) 2-5 years”
- life stage study.description · “that, thirty healthy volunteers from Valencian Community”
- life stage study.description · “old, and 10 elderly (E) 65-85 years”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA7100486 | age_at_collection_days | 9854 | R2 | 0.80 | paper.supp.41598_2026_35033_MOESM9_ESM.xls[SupplementaryTable2!age(days)] | 9854 |
| SAMEA7100485 | collection_date | 2017 | R1 | 0.90 | biosample.attribute:collection_date | 2017 |
| SAMEA7100485 | country | ES | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Spain |
| SAMEA7100485 | health_condition | healthy_control | R1 | 0.85 | biosample.attribute:project name | PRJEB39661 |
| SAMEA7100485 | health_condition_detail | PRJEB39661 | R1 | 0.85 | biosample.attribute:project name | PRJEB39661 |
| SAMEA7100485 | latitude | 39.4561 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 39.4561165 -0.3545661 |
| SAMEA7100485 | lifestyle | urban_industrialized | R2 | 0.75 | paper.supp.41598_2026_35033_MOESM8_ESM.xls[SupplementaryTable1!host_lifestyle] | Urban |
| SAMEA7100485 | location_region | Valencia | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 39.4561165 -0.3545661 |
| SAMEA7100485 | longitude | -0.3546 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 39.4561165 -0.3545661 |
| SAMEA7100485 | sex | female | R2 | 0.75 | paper.supp.41598_2026_35033_MOESM8_ESM.xls[SupplementaryTable1!host_sex] | Female |
| SAMEA7100485 | subject_id | A01 | R1 | 0.70 | sample.name_rule:alias | A01 |
| SAMEA7100485 | timepoint_label | T1 | R1 | 0.70 | sample.name_rule:alias | T1 |
Sequencing
304 runs over 304 samples · mean 0.25 Gbp per run (median 0.19 Gbp, total 75.90 Gbp; 304 runs with a base count) · layout PAIRED 304 · platform ILLUMINA 304 · models NextSeq 500 (304) · 1% of runs with a Sandpiper profile · first public 2023-06-19
Authors (11)
Ruiz-Ruiz S first · Piquer-Esteban S · Pérez-Rocher B · Pérez-Brocal V · Arnau V · Artacho A · Diaz W · Jiménez-Hernández N · Pons J · Castro JA · Moya A last
Downloads
PRJEB39661.csv.gz (8.2 KB) · parquet (81.2 KB) — all 304 samples, every field with route and confidence · PRJEB39661_determinations.csv.gz (22.8 KB) — 3,478 evidence rows