Diosmectite trial in UK/Netherlands diarrhea patients
170 fecal samples from adults with chronic functional diarrhea in the United Kingdom and Netherlands, collected in an intervention trial assessing diosmectite drug therapy. machine-written summary
170 samples · 760 runs · GB, NL · Adult · 2022 · 2.89 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 1,275 sample values (age, country, intervention, intervention detail, sex, stool consistency bristol, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 680 sample values (antibiotic exposure, collection date, health condition, health condition detail) · PMC8840705 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
35 people have samples in more than one arm.
Non-antibiotic pharmaceutical given as the study intervention (metformin design single_arm_before_after · population gi_infection_or_diarrhoea · Diosmectite 3g/sachet x3/day for 5 weeks in chronic diarrhea
“5 weeks administration of diosmectite” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal
35 of 35 people sampled more than once (170 samples; median 5, up to 5 per person) · follow-up median 34 d (max 34 d) · median interval between samples 7 d (time from timepoint labels).
Each row is one person (35 shown); dots are samples at days since that person's first sample.
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 170 | 100% | ||||
| country | 170 | 100% | 170 | |||
| health_condition | 170 | 100% | 170 | |||
| subject_id | 170 | 100% | 170 | |||
| age_at_collection_days | 170 | 100% | 170 | |||
| antibiotic_exposure | 170 | 100% | 170 | |||
| collection_date | 170 | 100% | 170 | |||
| health_condition_detail | 170 | 100% | 170 | |||
| intervention | 170 | 100% | 170 | |||
| intervention_detail | 170 | 100% | 170 | |||
| sex | 170 | 100% | 170 | |||
| timepoint_label | 170 | 100% | 170 | |||
| stool_consistency_bristol | 85 | 50% | 85 |
- Long-term diosmectite use does not alter the gut microbiota in adults with chronic diarrhea. 2022 PMID 35151268 PMC8840705 own_data
Archive description
Diosmectite, a natural colloidal clay, has been used worldwide for a number of approved indications, including the treatment of chronic functional diarrhea. Here, we used high-resolution whole metagenome shotgun sequencing to assess the impact of a 5 weeks administration of diosmectite (3g/sachet, 3 sachets/day) on the fecal microbiota of 35 adults with functional chronic diarrhea. Gut microbiota was not impacted by diosmectite administration. In particular, richness remained stable and no microbial species displayed a significant evolution. Segregating patients either by diosmectite response (non responder, early responder, late responder) or by nationality (Great-Britain or Netherlands) yielded the same results. We concluded that no microbiota-related physiological alterations are expected upon long-term treatment with diosmectite. Trial registration: clinicaltrials.gov NCT03045926.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “not alter the gut microbiota in adults”
- body site study.description · “sachets/day) on the fecal microbiota of 35”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- life stage study.title · “gut microbiota in adults with chronic diarrhea”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA12737997 | age_at_collection_days | 8766.0 | R1 | 0.85 | sample.attr.age | 24 |
| SAMEA12737997 | antibiotic_exposure | no | R3 | 0.70 | paper.fulltext.methods | Antibiotic agent intake within the month prior to baseline |
| SAMEA12737997 | collection_date | 2016-08/2017-05 | R3 | 0.70 | paper.fulltext.methods | inclusion took place from 24 August 2016 to 9 May 2017 |
| SAMEA12737997 | country | NL | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Netherlands |
| SAMEA12737997 | health_condition | gi_infection_or_diarrhoea | R3 | 0.70 | paper.fulltext.abstract | functional chronic diarrhea |
| SAMEA12737997 | health_condition_detail | functional chronic diarrhea (Rome IV criteria, BSS grade 6-7) | R3 | 0.70 | paper.fulltext.abstract | functional chronic diarrhea |
| SAMEA12737997 | intervention | no_intervention | R1 | 0.80 | biosample.attribute:visit | visit: D-30 |
| SAMEA12737997 | intervention_detail | day -30, before diosmectite treatment | R1 | 0.80 | biosample.attribute:visit | visit: D-30 |
| SAMEA12737997 | sex | female | R1 | 0.90 | sample.attr.gender | F |
| SAMEA12738000 | stool_consistency_bristol | 4 | R1 | 0.90 | biosample.attribute:bristol_score | 4 |
| SAMEA12737997 | subject_id | 1 | R1 | 0.85 | sample.attr.subject | 1 |
| SAMEA12737997 | timepoint_label | D-30 | R1 | 0.85 | sample.attr.visit | D-30 |
Sequencing
760 runs over 170 samples · mean 0.65 Gbp per run (median 0.67 Gbp, total 490.52 Gbp; 760 runs with a base count) · layout SINGLE 760 · platform ION_TORRENT 760 · models Ion Torrent Proton (760) · 0% of runs with a Sandpiper profile · first public 2022-01-31
Authors (12)
Da Silva K first · Guilly S · Thirion F · Le Chatelier E · Pons N · Roume H · Quinquis B · Ehrlich SD · Bekkat N · Mathiex-Fortunet H · Sokol H · Doré J last
Downloads
PRJEB43454.csv.gz (5.6 KB) · parquet (77.8 KB) — all 170 samples, every field with route and confidence · PRJEB43454_determinations.csv.gz (11.2 KB) — 1,955 evidence rows