FMT plus Mediterranean diet in metabolic syndrome, Netherlands
124 gut microbiome samples from male subjects with metabolic syndrome in the Netherlands, in a randomized controlled trial comparing lean donor versus autologous fecal microbiota transplantation combined with a Mediterranean diet. machine-written summary
124 samples · 124 runs · NL · Adult · 2021 · 5.30 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 846 sample values (collection date, country, latitude, location region, longitude, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 620 sample values (health condition, health condition detail, location locality, location site, sex) · PMC8222733 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Controlled / prescribed diet or feeding regimen Faecal microbiota transplantation design randomized_controlled_trial · population cardiometabolic_other · Mediterranean diet + lean donor vs autologous FMT, 6 weeks
“randomized to either lean donor (n=12) or autologous (n=12) FMT” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal
21 of 23 people sampled more than once (105 samples; median 5, up to 5 per person) · follow-up median 3 mo (max 3 mo) · median interval between samples 18 d (time from timepoint labels).
Each row is one person (21 shown); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 120 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 124 | 100% | ||||
| country | 124 | 100% | 124 | |||
| health_condition | 124 | 100% | 124 | |||
| subject_id | 107 | 86% | 107 | |||
| collection_date | 124 | 100% | 124 | |||
| detailed_location | 124 | 100% | ||||
| health_condition_detail | 124 | 100% | 124 | |||
| latitude | 124 | 100% | 124 | |||
| location_locality | 124 | 100% | 124 | |||
| location_region | 124 | 100% | 124 | |||
| location_site | 124 | 100% | 124 | |||
| longitude | 124 | 100% | 124 | |||
| sex | 124 | 100% | 124 | |||
| timepoint_label | 119 | 96% | 119 |
- Effect of Fecal Microbiota Transplantation Combined With Mediterranean Diet on Insulin Sensitivity in Subjects With Metabolic Syndrome. 2021 PMID 34177842 PMC8222733 own_data
Archive description
Background: Recent studies demonstrate that a Mediterranean diet has beneficial metabolic effects in metabolic syndrome subjects. Since we have shown that fecal microbiota transplantation (FMT) from lean donors exerts beneficial effects on insulin-sensitivity, in the present trial we investigated the potential synergistic effects on insulin-sensitivity of combining Mediterranean diet with donor FMT in subjects with metabolic syndrome.Design: Twenty-four male subjects with metabolic syndrome were put on a Mediterranean diet and after a two-week run-in phase, subjects were randomized to either lean donor (n=12) or autologous (n=12) FMT. Changes in gut microbiota composition and bacterial strain engraftment after 2-week dietary regimens and 6 weeks post-FMT were primary endpoints. Secondary objectives were changes in glucose fluxes (both hepatic and peripheral insulin sensitivity), postprandial plasma incretin (GLP-1) levels, subcutaneous adipose tissue inflammation and plasma metabolites.Results: Consumption of the Mediterranean diet resulted in a reduction in body weight, HOMA-IR and lipid levels. However, no large synergistic effects of combining the diet with lean donor FMT were seen on gut microbiota diversity after 6 weeks. Although we did observe changes in specific bacterial species and plasma metabolites, no significant beneficial effects on glucose fluxes, postprandial incretins or subcutaneous adipose tissue inflammation were detected.Conclusions: In this small pilot randomized controlled trial, no synergistic beneficial metabolic effects of combining Mediterranean diet with lean donor FMT on glucose metabolism were achieved. However, we observed engraftment of specific bacterial species. Future trials are warranted to test the combination of other microbial interventions and diets in metabolic syndrome.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.78. Assay shotgun_dna.
- host run.scientific_name · “human gut metagenome”
- body site run.scientific_name · “human gut metagenome”
- life stage study.description · “Twenty-four male subjects with metabolic syndrome”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA8690926 | collection_date | 2017 | R1 | 0.90 | biosample.attribute:collection_date | 2017 |
| SAMEA8690926 | country | NL | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Netherlands |
| SAMEA8690926 | health_condition | obesity | R3 | 0.70 | paper.fulltext.methods | obese treatment-naive Caucasian male subjects |
| SAMEA8690926 | health_condition_detail | obese treatment-naive Caucasian male subjects | R3 | 0.70 | paper.fulltext.methods | obese treatment-naive Caucasian male subjects |
| SAMEA8690926 | latitude | 52.2940 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 52.29398138409803 4.958415663249101 |
| SAMEA8690926 | location_locality | Amsterdam | R3 | 0.70 | paper.fulltext.methods | Amsterdam University Medical Center (Amsterdam UMC) in Amsterdam |
| SAMEA8690926 | location_region | North Holland | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 52.29398138409803 4.958415663249101 |
| SAMEA8690926 | location_site | Amsterdam UMC | R3 | 0.70 | paper.fulltext.methods | conducted at the AMC in accordance with the Declaration of Helsinki |
| SAMEA8690926 | longitude | 4.9584 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 52.29398138409803 4.958415663249101 |
| SAMEA8690926 | sex | male | R3 | 0.70 | paper.fulltext.methods | obese treatment-naive Caucasian male subjects |
| SAMEA8690927 | subject_id | D03 | R1 | 0.70 | sample.name_rule:alias | D03 |
| SAMEA8690931 | timepoint_label | week 0 (FMT baseline) | R1 | 0.70 | sample.name_rule:alias | M01_W0 |
Sequencing
124 runs over 124 samples · mean 5.19 Gbp per run (median 5.30 Gbp, total 643.83 Gbp; 124 runs with a base count) · layout PAIRED 124 · platform ILLUMINA 124 · models Illumina NovaSeq 6000 (124) · 97% of runs with a Sandpiper profile · first public 2021-06-11
Authors (18)
Koopen AM first · Almeida EL · Attaye I · Witjes JJ · Rampanelli E · Majait S · Kemper M · Levels JHM · Schimmel AWM · Herrema H · Scheithauer TPM · Frei W · Dragsted L · Hartmann B · Holst JJ · O'Toole PW · Groen AK · Nieuwdorp M last
Downloads
PRJEB44237.csv.gz (4.3 KB) · parquet (75.9 KB) — all 124 samples, every field with route and confidence · PRJEB44237_determinations.csv.gz (10.6 KB) — 1,466 evidence rows