Non-nutritive sweeteners, microbiome
120 healthy Israeli adults received saccharin, sucralose, aspartame, stevia, or control over 2 weeks in a randomized trial. Stool and oral microbiome samples (N=2015) assessed NNS impacts on glycemic responses and microbial composition. machine-written summary
1,869 samples · 2,018 runs · IL · Adult · 2022 · 0.18 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 13,702 sample values (collection date, country, intervention, intervention detail, latitude, location region, longitude, subject id) |
| ✓ Abstract / description | 3,738 sample values (health condition, health condition detail) |
| ○ Full text | read; no usable statement or table · PMC9879504 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
design randomized_controlled_trial · Saccharin, sucralose, aspartame, stevia sachets 2 weeks vs glucose/no supplement controls
longitudinal — not resolved
120 of 120 people sampled more than once (1,869 samples; median 16, up to 18 per person). Schedule stated by the source: “Repeated stool (S) and oral (O) sampling across a 2-week NNS intervention per subject, timepoint coded in sample title (e.g., O1, O14, S4, S17)” (time origin: intervention_start); stated follow-up 14 d.
Genera, mean relative abundance over 1,083 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 1,869 | 100% | ||||
| country | 1,869 | 100% | 1,869 | |||
| health_condition | 1,869 | 100% | 1,869 | |||
| subject_id | 1,869 | 100% | 1,869 | |||
| collection_date | 1,869 | 100% | 1,869 | |||
| health_condition_detail | 1,869 | 100% | 1,869 | |||
| timepoint_label | 1,869 | 100% | 1,869 | |||
| detailed_location | 1,866 | 100% | ||||
| latitude | 1,866 | 100% | 1,866 | |||
| location_region | 1,866 | 100% | 1,866 | |||
| longitude | 1,866 | 100% | 1,866 | |||
| intervention | 627 | 34% | 627 | |||
| intervention_detail | 1 | 0% | 1 |
- A convenient correspondence between k-mer-based metagenomic distances and phylogenetically-informed β-diversity measures. 2023 PMID 36608056 PMC9879504 own_data
Archive description
Non-nutritive sweeteners (NNS) are commonly integrated into human diet and presumed to be inert however, animal studies suggest that they may impact the microbiome and downstream glycemic responses. We causally assessed NNS impacts in humans and their microbiomes in a randomized-controlled trial encompassing 120 healthy adults, administered saccharin, sucralose, aspartame, and stevia sachets for 2 weeks in doses lower than the acceptable daily intake, compared with controls receiving sachet-contained vehicle glucose or no supplement. As groups, each administered NNS distinctly altered stool and oral microbiome and plasma metabolome, whereas saccharin and sucralose significantly impaired glycemic responses. Importantly, gnotobiotic mice conventionalized with microbiomes from multiple top and bottom responders of each of the four NNS-supplemented groups featured glycemic responses largely reflecting those noted in respective human donors, which were preempted by distinct microbial signals, as exemplified by sucralose. Collectively, human NNS consumption may induce person-specific, microbiome-dependent glycemic alterations, necessitating future assessment of clinical implications.
How this project entered the registry
Classified as human and non-human (mixed) gut / stool / oral cavity shotgun DNA metagenome of adult by opus_adjudicated with confidence 0.75. Assay shotgun_dna.
- host run.scientific_name · “Homo sapiens”
- host run.scientific_name · “Mus musculus”
- host study.description · “gnotobiotic mice conventionalized with microbiomes”
- body site study.description · “altered stool and oral microbiome”
- life stage study.description · “120 healthy adults”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA110651595 | collection_date | 2018 | R1 | 0.90 | biosample.attribute:collection_date | 2018 |
| SAMEA110651595 | country | IL | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Israel |
| SAMEA110651595 | health_condition | healthy_control | R4 | 0.50 | study.description | 120 healthy adults, administered saccharin, sucralose, aspartame, and stevia |
| SAMEA110651595 | health_condition_detail | randomized-controlled trial of non-nutritive sweeteners | R4 | 0.50 | study.description | randomized-controlled trial encompassing 120 healthy adults, administered saccharin, sucralose, aspartame, and stevia |
| SAMEA110651595 | intervention | placebo | R1 | 0.70 | sample.name_rule:description | Oral sample from participant D409 from Glucose vehicle group taken at day 14 |
| SAMEA110652132 | intervention_detail | Stevia (non-nutritive sweetener sachets) | R1 | 0.70 | sample.name_rule:description | Oral sample from participant S522 from Stevia group taken at day O1 |
| SAMEA110651595 | latitude | 31.9045 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 31.904496382 34.805496778 |
| SAMEA110651595 | location_region | Central District | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 31.904496382 34.805496778 |
| SAMEA110651595 | longitude | 34.8055 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 31.904496382 34.805496778 |
| SAMEA110651595 | subject_id | D409 | R1 | 0.70 | sample.name_rule:description | Oral sample from participant D409 from Glucose vehicle group taken at day 14 |
| SAMEA110651595 | timepoint_label | 14 | R1 | 0.70 | sample.name_rule:description | Oral sample from participant D409 from Glucose vehicle group taken at day 14 |
Sequencing
2,018 runs over 1,869 samples · mean 0.13 Gbp per run (median 0.18 Gbp, total 257.22 Gbp; 2,018 runs with a base count) · layout SINGLE 2,018 · platform ILLUMINA 2,018 · models NextSeq 550 (2,018) · 60% of runs with a Sandpiper profile · first public 2022-08-16 – 2022-09-10
Authors (2)
Zhai H first · Fukuyama J last
Downloads
PRJEB47383.csv.gz (31.7 KB) · parquet (116.0 KB) — all 1,869 samples, every field with route and confidence · PRJEB47383_determinations.csv.gz (131.7 KB) — 17,440 evidence rows