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PRJEB50473 ERP135051

PacBio HiFi metagenome of a healthy adult's gut, France

PacBio HiFi sequencing of a human fecal metagenome

Cross-sectional study of 1 fecal sample from a healthy adult donor in France, sequenced with PacBio HiFi technology, yielding 89 metagenome-assembled genomes including 9 complete circular genomes. machine-written summary

1 samples · 1 runs · FR · Age unknown · 2022 · 13.01 Gbp / sample

Sources
✓Sequence archive 7○Abstract / description✓Full text 4○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive7 sample values (collection date, country, latitude, location locality, location region, longitude, sex)
○ Abstract / descriptionread; no usable statement
✓ Full text4 sample values (age, health condition, health condition detail, subject id) · PMC9202402
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
adult1
Condition
healthy_control1
Sex
male1
Country
FR1
Body site
primary1
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)all projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 1 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 13.01 Gbp per sample0130Gbp
Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category1 100%
country1 100%1
health_condition1 100%1
subject_id1 100%1
age_at_collection_days1 100%1
collection_date1 100%1
detailed_location1 100%
health_condition_detail1 100%1
latitude1 100%1
location_locality1 100%1
location_region1 100%1
longitude1 100%1
sex1 100%1
Papers (1)
  • Recovery of Metagenome-Assembled Genomes from a Human Fecal Sample with Pacific Biosciences High-Fidelity Sequencing. 2022 PMID 35532226 PMC9202402 own_data
Details
Archive description

Characterization of the fecal metagenome from a healthy human donor with the PacBio HiFi sequencing technology.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.85. Assay shotgun_dna.

  • host study.description · “healthy human donor”
  • body site study.title · “human fecal metagenome”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMEA12616356age_at_collection_days10958R30.70paper.fulltext.methods30-year-old healthy French volunteer
SAMEA12616356collection_date2019R10.90biosample.attribute:collection_date2019
SAMEA12616356countryFRR10.90sample.attr.geographic_location_country_and_or_seaFrance
SAMEA12616356health_conditionhealthy_controlR30.70paper.fulltext.methodsself-collected by a 30-year-old healthy
SAMEA12616356health_condition_detailhealthy human donorR30.70paper.fulltext.methodsself-collected by a 30-year-old healthy
SAMEA12616356latitude48.8583R10.90biosample.attribute:geographic_location_latitude+geographic_location_longitude48.8583 2.2923
SAMEA12616356location_localityJouy-en-JosasR10.85biosample.attribute:geographic_location_region_and_localityJouy-en-Josas
SAMEA12616356location_regionIle-de-FranceR10.60biosample.attribute:geographic_location_latitude+geographic_location_longitude48.8583 2.2923
SAMEA12616356longitude2.2923R10.90biosample.attribute:geographic_location_latitude+geographic_location_longitude48.8583 2.2923
SAMEA12616356sexmaleR10.90sample.attr.host_sexmale
SAMEA12616356subject_idSAMEA12616356R30.70design.one_sample_per_subject:fulltextA fecal sample was self-collected by a 30-year-old healthy French volunteer for whom written consent was obtained.
Sequencing

1 runs over 1 samples · mean 13.01 Gbp per run (median 13.01 Gbp, total 13.01 Gbp; 1 runs with a base count) · layout SINGLE 1 · platform PACBIO_SMRT 1 · models Sequel (1) · 0% of runs with a Sandpiper profile · first public 2022-01-31

Authors (3)

Plaza Oñate F first · Roume H · Almeida M last

Downloads

PRJEB50473.csv.gz (941 B) · parquet (70.6 KB) — all 1 samples, every field with route and confidence · PRJEB50473_determinations.csv.gz (908 B) — 11 evidence rows

Samples (1 · every field this project has values for · hover a cell for its source)
Loading the sample table…