Healthy donor stool samples from UC FMT (LOTUS) study
31 shotgun metagenome samples from healthy donors in the LOTUS faecal microbiota transplantation study for ulcerative colitis, an intervention design investigating donor bacterial determinants of therapeutic outcome. machine-written summary
31 samples · 31 runs · Age unknown · 2022 · 15.45 Gbp / sample · cohort Fungal Trans-kingdom Dynamics Linked to Responsive
One study deposited as 2 BioProjects (252 catalog samples together): PRJEB50699 · PRJEB58035 · show together
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ○ Sequence archive | records read; no usable sample values |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 62 sample values (health condition, health condition detail) · PMC11406951, PMC11487140, PMC12507277, PMC12542657 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Faecal microbiota transplantation design randomized_controlled_trial · population ulcerative_colitis · Lyophilised oral FMT donor samples, double-blind RCT in ulcerative colitis
“double-blind randomized trials of FMT in ulcerative colitis” — abstract:39420033 (study level; per-sample arms are in the intervention column below)
Genera, mean relative abundance over 29 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 0 | 0% | ||||
| country | 0 | 0% | ||||
| health_condition | 31 | 100% | 31 | |||
| subject_id | 0 | 0% | ||||
| health_condition_detail | 31 | 100% | 31 |
- Refining microbial community metabolic models derived from metagenomics using reference-based taxonomic profiling. 2024 PMID 39136455 PMC11406951 own_data
- kMermaid: Ultrafast metagenomic read assignment to protein clusters by hashing of amino acid k-mer frequencies. 2025 PMID 40934284 PMC12507277 own_data
- The phageome of patients with ulcerative colitis treated with donor fecal microbiota reveals markers associated with disease remission. 2024 PMID 39420033 PMC11487140 unsure
- Bacterial taxonomic and functional changes following oral lyophilized donor fecal microbiota transplantation in patients with ulcerative colitis. 2025 PMID 40952001 PMC12542657 unsure
Archive description
Faecal microbiota transplantation (FMT) delivered via colonoscopic infusion or enemas have been shown to induce remission in a proportion of patients with active ulcerative colitis. Here, we aimed to investigate donor bacterial taxonomic and functional determinants of therapeutic outcome.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of age unknown by opus_adjudicated with confidence 0.80. Assay shotgun_dna.
- host run.scientific_name · “Homo sapiens”
- body site study.title · “oral faecal microbiota transplantation”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA12869768 | health_condition | healthy_control | R3 | 0.70 | paper.fulltext.methods | stool samples from healthy individuals was part of a study |
| SAMEA12869768 | health_condition_detail | healthy individuals ... donors of the study | R3 | 0.70 | paper.fulltext.methods | stool samples from healthy individuals was part of a study |
Sequencing
31 runs over 31 samples · mean 14.41 Gbp per run (median 15.45 Gbp, total 446.58 Gbp; 31 runs with a base count) · layout PAIRED 31 · platform ILLUMINA 31 · models Illumina NovaSeq 6000 (31) · 94% of runs with a Sandpiper profile · first public 2022-07-27
Authors (16)
Majzoub ME first · Luu LDW · Haifer C · Paramsothy S · Borody TJ · Leong RW · Thomas T · Kaakoush NO last · Parthasarathy R · Kamm MA · Lucas A first · Schäffer DE · Wickramasinghe J · Auslander N last · Raich SS first · Shamim MMI
Downloads
PRJEB50699.csv.gz (1.6 KB) · parquet (69.5 KB) — all 31 samples, every field with route and confidence · PRJEB50699_determinations.csv.gz (691 B) — 62 evidence rows