Gut microbiota in anorexia nervosa, case-control, Denmark
147 faecal samples from adult and adolescent females in Denmark, comparing anorexia nervosa cases with healthy controls in a case-control design, including fecal microbiota transplantation experiments. machine-written summary
147 samples · 594 runs · DK · Adult · 2023 · 2.98 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 147 sample values (country) |
| ○ Abstract / description | read; no usable statement |
| ○ Full text | read; no usable statement or table · PMC10159860 |
| ✓ Supplementary tables | 147 sample values (subject id) |
| ✓ External resources | 873 sample values (age, antibiotic exposure, bmi, health condition, health condition detail, sex) · from curatedMetagenomicData 3 (cMD 3) |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Faecal microbiota transplantation design observational_with_procedure · population other_disease · FMT from AN patients to germ-free mice (animal experiment)
“faecal microbiota transplantation from AN cases to germ-free mice” — abstract:37069399 (study level; per-sample arms are in the intervention column below)
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 147 | 100% | ||||
| country | 147 | 100% | 147 | |||
| health_condition | 147 | 100% | 147 | |||
| subject_id | 147 | 100% | 147 | |||
| antibiotic_exposure | 147 | 100% | 147 | |||
| bmi | 147 | 100% | 147 | |||
| health_condition_detail | 147 | 100% | 147 | |||
| sex | 147 | 100% | 147 | |||
| age_at_collection_days | 138 | 94% | 138 |
- The gut microbiota contributes to the pathogenesis of anorexia nervosa in humans and mice. 2023 PMID 37069399 PMC10159860 own_data
Archive description
Anorexia nervosa (AN) is an eating disorder with a high mortality affecting about 1% of women, where no evidence-based effective treatment exists. The pathogenesis likely involves genetic and environmental alterations. We hypothesized that a disrupted gut microbiota contributes to AN pathogenesis. In analyses comparing 70 AN with 77 healthy females, we found multiple taxa, functional modules, structural variants and growth rates of bacterial gut microbiota, and viral gut microbiota that were altered in AN with parts of these perturbations linked to estimates of eating behavior and mental health. In silico, causal inference analyses implied bacterial metabolites mediated parts of the impact of altered gut microbiota on AN behavior, and in vivo, fecal microbiota transplantation from AN cases to germ-free mice induced a lower body weight and hypothalamic and adipose tissue gene expressions related to aberrant energy metabolism and eating and mental behavior.
How this project entered the registry
Classified as human gut / stool / blood / tissue / internal fluids shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Disrupted intestinal microbiota contributes to”
- body site study.description · “that a disrupted gut microbiota contributes to”
- body site study.description · “hypothalamic and adipose tissue gene expressions related”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- life stage study.description · “about 1% of women , where no”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA112657319 | age_at_collection_days | 5844.0 | R2 | 0.75 | external.cmd source | age: 16; age_unit: (empty); age_group: Adolescent |
| SAMEA112657319 | antibiotic_exposure | no | R2 | 0.75 | external.cmd source | antibiotics_current_use: No |
| SAMEA112657319 | bmi | 18.1 | R2 | 0.75 | external.cmd source | bmi: 18.1 |
| SAMEA112657319 | country | DK | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Denmark |
| SAMEA112657319 | health_condition | other_disease | R2 | 0.75 | external.cmd source | disease: Anorexia |
| SAMEA112657319 | health_condition_detail | Anorexia | R2 | 0.75 | external.cmd source | disease: Anorexia |
| SAMEA112657319 | sex | female | R2 | 0.75 | external.cmd source | sex: Female |
| SAMEA112657319 | subject_id | DKAN_01 | R2 | 0.80 | paper.supp.41564_2023_1355_MOESM13_ESM.xlsx[5a!ID] | DKAN_01 |
Sequencing
594 runs over 147 samples · mean 0.76 Gbp per run (median 0.72 Gbp, total 453.93 Gbp; 594 runs with a base count) · layout SINGLE 594 · platform ION_TORRENT 594 · models Ion Torrent Proton (594) · 0% of runs with a Sandpiper profile · first public 2023-02-18
Authors (26)
Fan Y first · Støving RK · Berreira Ibraim S · Hyötyläinen T · Thirion F · Arora T · Lyu L · Stankevic E · Hansen TH · Déchelotte P · Sinioja T · Ragnarsdottir O · Pons N · Galleron N · Quinquis B · Levenez F · Roume H · Falony G · Vieira-Silva S · Raes J · Clausen L · Telléus GK · Bäckhed F · Oresic M · Ehrlich SD · Pedersen O last
Downloads
PRJEB51776.csv.gz (5.3 KB) · parquet (76.9 KB) — all 147 samples, every field with route and confidence · PRJEB51776_determinations.csv.gz (13.7 KB) — 1,167 evidence rows