Prebiotic fiber crossover trial in healthy US adults
140 fecal/plasma samples from healthy adults in the USA in a randomized, placebo-controlled, double-blind crossover trial testing a prebiotic dietary fiber blend versus placebo on gut microbiota and metabolomics. machine-written summary
140 samples · 140 runs · US · Adult · 2022 · 0.33 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 839 sample values (country, latitude, location region, longitude, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 700 sample values (antibiotic exposure, collection date, health condition, health condition detail, location locality) · PMC9248813 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Prebiotic / dietary fibre supplement design crossover · population healthy_control · 12 g prebiotic dietary fiber blend daily for 4 weeks
“consumed 12 g of a prebiotic fiber supplement” — abstract:35782954 (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
20 of 20 people sampled more than once (140 samples; median 7, up to 7 per person). Schedule stated by the source: “Each of 20 subjects sampled at 7 timepoints (A-G) across placebo and prebiotic arms of a crossover design with 4-week treatment periods and 4-week washout.”.
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 140 | 100% | ||||
| country | 140 | 100% | 140 | |||
| health_condition | 140 | 100% | 140 | |||
| subject_id | 140 | 100% | 140 | |||
| antibiotic_exposure | 140 | 100% | 140 | |||
| collection_date | 140 | 100% | 140 | |||
| detailed_location | 140 | 100% | ||||
| health_condition_detail | 140 | 100% | 140 | |||
| latitude | 140 | 100% | 140 | |||
| location_locality | 140 | 100% | 140 | |||
| location_region | 140 | 100% | 140 | |||
| longitude | 140 | 100% | 140 | |||
| timepoint_label | 139 | 99% | 139 |
- (no title) PMID 35559516 own_data
- Multi-Omic Analyses Reveal Bifidogenic Effect and Metabolomic Shifts in Healthy Human Cohort Supplemented With a Prebiotic Dietary Fiber Blend. 2022 PMID 35782954 PMC9248813 own_data
Archive description
The objective of this project is to conduct a randomized-order, double-blinded cross-over trial in 20 participants to test the effects of a dietary fiber formulation on gut microbiota composition and short chain fatty acid production, lipid profiles, glucose sensitivity, intestinal permeability, overall gut health, and markers of inflammation.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.70. Assay shotgun_dna.
- host study.description · “20 participants”
- body site study.description · “gut microbiota composition”
- life stage study.title · “Healthy Human Cohort”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA14404158 | antibiotic_exposure | no | R3 | 0.70 | paper.fulltext.methods | use of antibiotics within 6 months prior to study commencement |
| SAMEA14404158 | collection_date | 2019-04/2019-12 | R3 | 0.70 | paper.fulltext.methods | Recruitment began in April of 2019 |
| SAMEA14404158 | country | US | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | USA |
| SAMEA14404158 | health_condition | healthy_control | R3 | 0.70 | paper.fulltext.methods | Twenty healthy men and women aged 18–45 y |
| SAMEA14404158 | health_condition_detail | Generally healthy young participants consuming a low-fiber diet | R3 | 0.60 | paper.fulltext.methods | Twenty healthy men and women aged 18–45 y |
| SAMEA14404158 | latitude | 38.5467 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 38.546719 _121.744339 |
| SAMEA14404158 | location_locality | Davis | R3 | 0.70 | paper.fulltext.methods | University of California (UC), Davis |
| SAMEA14404158 | location_region | California | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 38.546719 _121.744339 |
| SAMEA14404158 | longitude | -121.7443 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 38.546719 _121.744339 |
| SAMEA14404158 | subject_id | 201 | R1 | 0.70 | sample.name_rule:title | 201A |
| SAMEA14404158 | timepoint_label | A | R1 | 0.70 | sample.name_rule:title | 201A |
Sequencing
140 runs over 140 samples · mean 0.32 Gbp per run (median 0.33 Gbp, total 44.61 Gbp; 140 runs with a base count) · layout SINGLE 140 · platform ILLUMINA 140 · models Illumina NovaSeq 6000 (140) · 0% of runs with a Sandpiper profile · first public 2022-05-18 – 2022-05-19
Authors (9)
Kang JW first · Tang X · Walton CJ · Brown MJ · Brewer RA · Maddela RL · Zheng JJ · Agus JK · Zivkovic AM last
Downloads
PRJEB52881.csv.gz (4.2 KB) · parquet (75.6 KB) — all 140 samples, every field with route and confidence · PRJEB52881_determinations.csv.gz (9.9 KB) — 1,539 evidence rows