L. reuteri probiotic trial in elderly women, Sweden
38 samples from older Swedish women with low bone mineral density, comparing good and poor responders to one-year Lactobacillus reuteri ATCC PTA 6475 supplementation; case-control design. machine-written summary
38 samples · 38 runs · SE · Adult · 2022 · 7.44 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 114 sample values (collection date, country, subject id) |
| ✓ Abstract / description | 76 sample values (health condition, health condition detail) |
| ○ Full text | read; no usable statement or table · PMC9581899 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Probiotic (live microorganisms; single strain or mixture) design randomized_controlled_trial · population other_disease · Lactobacillus reuteri ATCC PTA 6475, daily supplementation, 1 year
“supplementation of Lactobacillus reuteri ATCC PTA 6475 reduced bone loss” — abstract:36261538 (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
18 of 20 people sampled more than once (36 samples; median 2, up to 2 per person). Schedule stated by the source: “fecal samples at baseline and at 12 months after one-year probiotic supplementation” (time origin: intervention_start); stated follow-up 12 mo.
Genera, mean relative abundance over 34 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 38 | 100% | ||||
| country | 38 | 100% | 38 | |||
| health_condition | 38 | 100% | 38 | |||
| subject_id | 38 | 100% | 38 | |||
| collection_date | 38 | 100% | 38 | |||
| health_condition_detail | 38 | 100% | 38 |
- One-year supplementation with Lactobacillus reuteri ATCC PTA 6475 counteracts a degradation of gut microbiota in older women with low bone mineral density. 2022 PMID 36261538 PMC9581899 own_data
Archive description
Recent studies have shown that probiotic supplementation has beneficial effects on bone metabolism. In a randomized controlled trial (RCT) we demonstrated that supplementation of Lactobacillus reuteri ATCC PTA 6475 reduced bone loss in older women with low bone mineral density. To investigate the mechanisms underlying the effect of L. reuteri ATCC PTA 6475 on bone metabolism, 20 women with the highest changes (good responders) and the lowest changes (poor responders) in tibia total volumetric BMD after one-year supplementation were selected from our previous RCT. In the current study we characterized the gut microbiome composition and function as well as serum metabolome in good responders and poor responders to the probiotic treatment as a secondary analysis.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult and elderly by sonnet_x2 with confidence 0.68. Assay shotgun_dna.
- host run.scientific_name · “Homo sapiens”
- body site study.description · “characterized the gut microbiome composition”
- life stage study.title · “older women with low bone mineral density”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA14418825 | collection_date | 2019 | R1 | 0.90 | biosample.attribute:collection_date | 2019 |
| SAMEA14418825 | country | SE | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Sweden |
| SAMEA14418825 | health_condition | other_disease | R4 | 0.50 | study.description | low bone mineral density |
| SAMEA14418825 | health_condition_detail | Low bone mineral density; secondary analysis of RCT. | R4 | 0.50 | study.description | low bone mineral density |
| SAMEA14418825 | subject_id | 18 | R1 | 0.70 | sample.name_rule:alias | D18MO2167 |
Sequencing
38 runs over 38 samples · mean 7.40 Gbp per run (median 7.44 Gbp, total 281.16 Gbp; 38 runs with a base count) · layout PAIRED 38 · platform ILLUMINA 38 · models Illumina NovaSeq 6000 (38) · 89% of runs with a Sandpiper profile · first public 2022-05-21
Downloads
PRJEB52923.csv.gz (1.8 KB) · parquet (70.4 KB) — all 38 samples, every field with route and confidence · PRJEB52923_determinations.csv.gz (1.9 KB) — 190 evidence rows