Dietary bioactive crossover intervention in UK adults
100 gut microbiome samples from healthy adults in the United Kingdom, studied in a randomised 2x2 cross-over intervention comparing diets high versus low in bioactive-rich foods, with a four-week washout between periods. machine-written summary
100 samples · 100 runs · GB · Adult · 2023 · 12.16 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 1,420 sample values (age, collection date, country, diet, diet detail, health condition, health condition detail, intervention) |
| – Abstract / description | no abstract or project description |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
Controlled / prescribed diet or feeding regimen design crossover · population healthy_control · 2x2 crossover: high vs low bioactive-rich diet for two weeks each, 4-week washout
“randomised 2x2 cross-over human intervention where healthy participants” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal
20 of 21 people sampled more than once (99 samples; median 5, up to 5 per person) · follow-up median 2 mo (max 3 mo) · median interval between samples 15 d (time from collection dates). Schedule stated by the source: “2x2 crossover with two 2-week diet interventions (high bioactive, low bioactive) and 4-week washout between interventions” (time origin: intervention start).
Each row is one person (20 shown); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 94 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 100 | 100% | ||||
| country | 100 | 100% | 100 | |||
| health_condition | 100 | 100% | 100 | |||
| subject_id | 100 | 100% | 100 | |||
| age_at_collection_days | 100 | 100% | 100 | |||
| collection_date | 100 | 100% | 100 | |||
| detailed_location | 100 | 100% | ||||
| health_condition_detail | 100 | 100% | 100 | |||
| latitude | 100 | 100% | 100 | |||
| location_region | 100 | 100% | 100 | |||
| longitude | 100 | 100% | 100 | |||
| sex | 100 | 100% | 100 | |||
| timepoint_label | 100 | 100% | 100 | |||
| diet | 80 | 80% | 80 | |||
| diet_detail | 80 | 80% | 80 | |||
| intervention | 80 | 80% | 80 | |||
| intervention_detail | 80 | 80% | 80 |
- Dietary bioactives increase gut microbiome diversity and alter host and microbial metabolite profiles 2025 own_data
Archive description
The DIME study consists of a randomised 2x2 cross-over human intervention where healthy participants (n = 20) are subjected to a diet high in bioactive-rich food for two weeks and a diet low in bioactive-rich food. There is a four-week washout between the two interventions.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “dietary bioactive on gut microbiome diversity (DIME)”
- body site external_curation.infant_catalog · “body_site_call stool”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA110758230 | age_at_collection_days | 16791.5 | R1 | 0.85 | sample.attr.host_age | 46 |
| SAMEA110758230 | collection_date | 2021-04-21 | R1 | 0.90 | biosample.attribute:collection_date | 2021-04-21 |
| SAMEA110758230 | country | GB | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | United Kingdom |
| SAMEA110758231 | diet | therapeutic_or_study_diet | R1 | 0.90 | biosample.attribute:host_diet | Before Low Bioactive Diet |
| SAMEA110758231 | diet_detail | Before Low Bioactive Diet | R1 | 0.90 | biosample.attribute:host_diet | Before Low Bioactive Diet |
| SAMEA110758230 | health_condition | healthy_control | R1 | 0.70 | biosample.name_rule:TITLE | Sample_408170-DIME01-MP |
| SAMEA110758230 | health_condition_detail | healthy participants (project description) | R1 | 0.70 | biosample.name_rule:TITLE | Sample_408170-DIME01-MP |
| SAMEA110758231 | intervention | no_intervention | R1 | 0.90 | biosample.attribute:host diet | host diet: Before Low Bioactive Diet |
| SAMEA110758231 | intervention_detail | baseline before low-bioactive-food diet period | R1 | 0.90 | biosample.attribute:host diet | host diet: Before Low Bioactive Diet |
| SAMEA110758230 | latitude | 52.6211 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 52.62109 1.21814 |
| SAMEA110758230 | location_region | England | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 52.62109 1.21814 |
| SAMEA110758230 | longitude | 1.2181 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 52.62109 1.21814 |
| SAMEA110758230 | sex | female | R1 | 0.90 | sample.attr.host_sex | female |
| SAMEA110758230 | subject_id | DIME01 | R1 | 0.85 | sample.attr.host_subject_id | DIME01 |
| SAMEA110758230 | timepoint_label | Midpoint | R1 | 0.85 | biosample.attribute:host diet | Midpoint |
Sequencing
100 runs over 100 samples · mean 11.97 Gbp per run (median 12.16 Gbp, total 1,196.52 Gbp; 100 runs with a base count) · layout PAIRED 100 · platform ILLUMINA 100 · models Illumina NovaSeq 6000 (100) · 94% of runs with a Sandpiper profile · first public 2023-03-22
Authors (13)
Duncan A first · Bernuzzi F · Ahn-Jarvis J · Zhang L · Segovia-Lizano D · Petrou M · Bedarf JR · Ng D · Savva GM · Stanstrup J · Dragsted LO · Hildebrand F · Traka MH last
Downloads
PRJEB55607.csv.gz (4.4 KB) · parquet (76.8 KB) — all 100 samples, every field with route and confidence · PRJEB55607_determinations.csv.gz (11.4 KB) — 1,420 evidence rows