Multi-body-site microbiome of Venezuelan Amerindian villages
226 longitudinal samples from nose, mouth, skin, and feces of Yekwana and Yanomami-Sanema villagers in Southern Bolívar, Venezuela, comparing low- and medium-exposure villages before and after Ivermectin treatment. machine-written summary
226 samples · 226 runs · VE · Age unknown · 2023 · 1.90 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 2,287 sample values (age, collection date, country, family id, latitude, location locality, longitude, sex) |
| ✓ Abstract / description | 678 sample values (health condition, health condition detail, location region) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
sex differs between samples of the same subject id (13 subjects, 38 samples) — sex withheld; subject ids shared by samples of both sexes (12 ids, 34 samples) — ids withheld.
The sources disagree on 46 samples. Where two incompatible values exist for one sample (or one person's samples disagree on sex), neither value is published: the field is left empty here, in the sample sheet and in the downloads. Both sides, with their sources, are in gut_metadata_contradictions.parquet of the data package (what counts as a contradiction).
Contradicted values (40 of 72)
| sample | field | value | other value | handling |
|---|---|---|---|---|
| SAMEA111265075 | sex | female (sequence archive) | subject 2015_11: female in 2 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265077 | sex | male (sequence archive) | subject 2015_13: female in 3 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265079 | sex | female (sequence archive) | subject 2015_15: male in 2 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265080 | sex | male (sequence archive) | subject 2015_16: female in 3 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265083 | sex | male (sequence archive) | subject 2015_20: male in 2 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265084 | sex | female (sequence archive) | subject 2015_21: female in 2 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265086 | sex | male (sequence archive) | subject 2015_31: male in 1 sample, female in 1 sample | withheld: neither value published |
| SAMEA111265090 | sex | female (sequence archive) | subject 2015_6: female in 2 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265091 | sex | female (sequence archive) | subject 2015_76: female in 1 sample, male in 1 sample | withheld: neither value published |
| SAMEA111265092 | sex | male (sequence archive) | subject 2015_9: male in 3 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265095 | sex | female (sequence archive) | subject 2015_13: female in 3 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265096 | sex | female (sequence archive) | subject 2015_16: female in 3 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265099 | sex | female (sequence archive) | subject 2015_6: female in 2 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265102 | sex | male (sequence archive) | subject 2015_9: male in 3 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265104 | sex | female (sequence archive) | subject 2015_11: female in 2 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265106 | sex | male (sequence archive) | subject 2015_11: female in 2 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265107 | sex | female (sequence archive) | subject 2015_13: female in 3 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265108 | sex | male (sequence archive) | subject 2015_15: male in 2 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265109 | sex | female (sequence archive) | subject 2015_16: female in 3 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265110 | sex | female (sequence archive) | subject 2015_17: female in 1 sample, male in 1 sample | withheld: neither value published |
| SAMEA111265111 | sex | female (sequence archive) | subject 2015_18: female in 1 sample, male in 1 sample | withheld: neither value published |
| SAMEA111265113 | sex | male (sequence archive) | subject 2015_21: female in 2 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265119 | sex | male (sequence archive) | subject 2015_28: male in 2 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265123 | sex | male (sequence archive) | subject 2015_9: male in 3 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265128 | sex | female (sequence archive) | subject 2015_13: female in 3 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265131 | sex | male (sequence archive) | subject 2015_28: male in 2 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265134 | sex | male (sequence archive) | subject 2015_17: female in 1 sample, male in 1 sample | withheld: neither value published |
| SAMEA111265136 | sex | female (sequence archive) | subject 2015_20: male in 2 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265140 | sex | male (sequence archive) | subject 2015_20: male in 2 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265141 | sex | female (sequence archive) | subject 2015_21: female in 2 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265160 | sex | male (sequence archive) | subject 2015_15: male in 2 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265161 | sex | female (sequence archive) | subject 2015_16: female in 3 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265162 | sex | male (sequence archive) | subject 2015_18: female in 1 sample, male in 1 sample | withheld: neither value published |
| SAMEA111265163 | sex | male (sequence archive) | subject 2015_76: female in 1 sample, male in 1 sample | withheld: neither value published |
| SAMEA111265168 | sex | male (sequence archive) | subject 2015_6: female in 2 samples, male in 1 sample | withheld: neither value published |
| SAMEA111265175 | sex | female (sequence archive) | subject 2015_28: male in 2 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265178 | sex | female (sequence archive) | subject 2015_31: male in 1 sample, female in 1 sample | withheld: neither value published |
| SAMEA111265180 | sex | female (sequence archive) | subject 2015_9: male in 3 samples, female in 1 sample | withheld: neither value published |
| SAMEA111265075 | subject id | 2015_11 | female (sequence archive) | subject id withheld (samples of both sexes share it) |
| SAMEA111265079 | subject id | 2015_15 | female (sequence archive) | subject id withheld (samples of both sexes share it) |
longitudinalcommunityfamilieshouseholdstribe
16 of 166 people sampled more than once (42 samples; median 2, up to 4 per person) · follow-up median 12.5 y (max 53.0 y) · median interval between samples 6.0 y (time from age at collection). Schedule stated by the source: “Subjects recruited/sampled in 2015 (before low-exposure villages' Ivermectin treatment) and again in 2016 (after all villages received Ivermectin treatment).”.
Each row is one person (16 shown); dots are samples at days since that person's first sample; rows of one family are adjacent and coloured by role (mother, father, child, twin).
Yekwana/Sanema villagers: 28 families, 28 houses, 8 villages, 2 ethnic groups (Family_ID, House_ID, Village, Ethnicity) · 28 families with 116 sampled people · 35 shared settings.
member · circles are people (pale = no sequenced sample), arrows run donor → recipient and parent → child, curved lines join twins, partners and siblings, squares are shared settings or families without recorded roles. Shown: 24 of 28 families (one per family structure, then more) · 8 of 35 shared settings. 166 people in the project overall.
Families (25 of 28)
Genera, mean relative abundance over 201 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 226 | 100% | ||||
| country | 226 | 100% | 226 | |||
| health_condition | 226 | 100% | 226 | |||
| subject_id | 192 | 85% | 192 | |||
| age_at_collection_days | 226 | 100% | 226 | |||
| detailed_location | 226 | 100% | ||||
| health_condition_detail | 226 | 100% | 226 | |||
| latitude | 226 | 100% | 226 | |||
| location_locality | 226 | 100% | 226 | |||
| location_region | 226 | 100% | 226 | |||
| longitude | 226 | 100% | 226 | |||
| shared_setting_id | 226 | 100% | 226 | |||
| shared_setting_type | 226 | 100% | 226 | |||
| collection_date | 206 | 91% | 206 | |||
| sex | 188 | 83% | 188 | |||
| family_id | 119 | 53% | 119 |
Archive description
Amerindians from Yekwana and Yanomami-Sanema villages in a region of 100 sq Km in Southern Bolivar State of Venezuela were recruited. There were 7 small villages which had low exposure to visitors and medicines and 1 village which had medium exposure to Western visitors and medicines (a medical post built in this village supplying medicines, antibiotics, antiparasitic and vaccines). In total, 118 subjects from low exposure villages and 79 subjects from medium exposure village were recruited in 2015, before the low exposure villages started Ivermectin treatment 161 subjects from low exposure villages and 74 subjects from medium exposure village were recruited in 2016, after all villages received Ivermectin treatment (see Table S5 for details). Samples were taken from nose, mouth, skin (on the right arm and right hand), and feces by swabbing, and were immediately frozen in dry shippers in the field.
How this project entered the registry
Classified as human gut / stool / oral cavity / skin / nasal / nasopharyngeal / multiple sites shotgun DNA metagenome of adult and child and adolescent by sonnet_x2 with confidence 0.68. Assay shotgun_dna.
- host study.description · “Amerindians from Yekwana and Yanomami-Sanema villages”
- body site study.description · “Samples were taken from nose, mouth, skin”
- body site study.description · “and feces by swabbing”
- life stage sample.attr.age · “24 (n=93/226)”
- life stage sample.attr.age · “8 (n=88/226)”
- life stage sample.attr.age · “13 (n=24/226)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA111265063 | age_at_collection_days | 4018.0 | R1 | 0.85 | sample.attr.age | 11 |
| SAMEA111265063 | collection_date | 2015-10-06 | R1 | 0.90 | biosample.attribute:collection_date | 2015-10-06 |
| SAMEA111265063 | country | VE | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Venezuela |
| SAMEA111265063 | family_id | 2015_1 | R1 | 0.85 | biosample.attribute:Family_ID | Family_ID: 2015_1 |
| SAMEA111265063 | health_condition | healthy_control | R4 | 0.50 | study.description | before the low exposure villages started Ivermectin treatment |
| SAMEA111265063 | health_condition_detail | Amerindian communities receiving Ivermectin treatment intervention | R4 | 0.50 | study.description | before the low exposure villages started Ivermectin treatment |
| SAMEA111265063 | latitude | 4.6700 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 4.67 -64.53 |
| SAMEA111265063 | location_locality | Chajuranha | R1 | 0.85 | biosample.attribute:village | Chajuranha |
| SAMEA111265063 | location_region | Bolivar State | R4 | 0.45 | ena.study_description | Southern Bolivar State of Venezuela |
| SAMEA111265063 | longitude | -64.5300 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 4.67 -64.53 |
| SAMEA111265063 | sex | male | R1 | 0.90 | sample.attr.gender | M |
| SAMEA111265063 | shared_setting_id | 2015_1 | R1 | 0.85 | biosample.attribute:House_ID;Village;Ethnicity | House_ID: 2015_1; Village: Chajuranha; Ethnicity: SANEMA |
| SAMEA111265066 | shared_setting_id | Chajuranha | R1 | 0.85 | biosample.attribute:Village;Ethnicity | Village: Chajuranha; Ethnicity: SANEMA |
| SAMEA111265063 | shared_setting_type | household | R1 | 0.85 | biosample.attribute:House_ID;Village;Ethnicity | House_ID: 2015_1; Village: Chajuranha; Ethnicity: SANEMA |
| SAMEA111265066 | shared_setting_type | village_or_community | R1 | 0.85 | biosample.attribute:Village;Ethnicity | Village: Chajuranha; Ethnicity: SANEMA |
| SAMEA111265063 | subject_id | 2015_1.10 | R1 | 0.85 | sample.attr.subject_id | 2015_1.10 |
Sequencing
226 runs over 226 samples · mean 2.30 Gbp per run (median 1.90 Gbp, total 518.75 Gbp; 226 runs with a base count) · layout PAIRED 226 · platform ILLUMINA 226 · models Illumina HiSeq 2000 (226) · 89% of runs with a Sandpiper profile · first public 2023-03-31
Authors (0)
No author record.
Downloads
PRJEB55648.csv.gz (9.1 KB) · parquet (83.7 KB) — all 226 samples, every field with route and confidence · PRJEB55648_determinations.csv.gz (22.6 KB) — 2,965 evidence rows