Psychobiotic diet intervention in healthy adults, Ireland
380 fecal/plasma/urine samples from a randomized trial of 45 healthy Irish adults (24 psychobiotic diet, 21 control) over 4 weeks, assessing gut microbiota, metabolites, and perceived stress. machine-written summary
380 samples · 380 runs · IE · Adult · 2022 · 0.30 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 1,304 sample values (collection date, country, intervention, subject id) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 1,520 sample values (antibiotic exposure, health condition, health condition detail, location locality) · PMC9908549 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Controlled / prescribed diet or feeding regimen design randomized_controlled_trial · population healthy_control · Psychobiotic diet (high prebiotic/fermented foods) vs control diet for 4 weeks
“randomized into either a psychobiotic (n = 24) or control (n =” — abstract:36289300 (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
47 of 47 people sampled more than once (368 samples; median 8, up to 8 per person). Schedule stated by the source: “Samples collected pre- and post- a 4-week dietary intervention (baseline and ~4 weeks later)” (time origin: intervention start); stated follow-up 28 d.
Genera, mean relative abundance over 311 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 380 | 100% | ||||
| country | 380 | 100% | 380 | |||
| health_condition | 380 | 100% | 380 | |||
| subject_id | 368 | 97% | 368 | |||
| antibiotic_exposure | 380 | 100% | 380 | |||
| collection_date | 380 | 100% | 380 | |||
| detailed_location | 380 | 100% | ||||
| health_condition_detail | 380 | 100% | 380 | |||
| location_locality | 380 | 100% | 380 | |||
| intervention | 176 | 46% | 176 |
- Feed your microbes to deal with stress: a psychobiotic diet impacts microbial stability and perceived stress in a healthy adult population. 2023 PMID 36289300 PMC9908549 own_data
Archive description
Both, the impact of diet on the microbiota composition as well as the regulation of the microbiota of brain processes, have been well established in the last decade. Additionally, there has been increasing emphasis on the role of diet in supporting optimal mental health. At the same time, psychobiotic interventions are emerging, salvaging the fact that the microbiota is modifiable by external factors. While probiotic and prebiotic interventions have shown promise as psychobiotic agents, whether whole dietary approaches can exert similar effects is largely understudied. Thus, we investigated the influence of a psychobiotic diet on the microbial profile and function as well as on stress and mood in a healthy human population. Forty five adults were randomized into either following a psychobiotic (n=24) or control (n=21) diet for 4 weeks. Microbiota composition and function was characterized using shotgun sequencing. Stress, mood, overall health and diet were assessed using standardized questionnaires. Metabolic profiling of plasma, urine and fecal samples was performed. Intervention with a psychobiotic diet resulted in reductions of perceived stress and an improvement in sleep quality. Additionally, higher adherence to the psychobiotic diet resulted in stronger decreases in perceived stress. Only subtle microbial compositional and functional changes were observed, but the dietary intervention elicited significant changes in the level of specific lipids in the faeces and urinary tryptophan metabolites. Lastly, microbial volatility was linked to greater changes in perceived stress scores in those on the psychobiotic diet. This data highlights that dietary approaches can be used to reduce stress in a human cohort, which holds promise in reducing the prevalence of stress-associated chronic diseases. However, additional research is warranted to investigate the role of the microbiota. Nevertheless, using healthy diets to positively modulate gut–brain communication holds possibilities for the reduction of stress and stress-associated disorders.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.75. Assay shotgun_dna.
- host run.scientific_name · “Homo sapiens”
- body site study.description · “plasma, urine and fecal samples”
- life stage study.description · “Forty five adults were randomized”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA111391305 | antibiotic_exposure | no | R3 | 0.70 | paper.fulltext.methods | had taken any pro-, pre- or antibiotic 4 weeks prior |
| SAMEA111391305 | collection_date | 2019 | R1 | 0.90 | biosample.attribute:collection_date | 2019 |
| SAMEA111391305 | country | IE | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Ireland |
| SAMEA111391305 | health_condition | healthy_control | R3 | 0.70 | paper.fulltext.methods | Healthy adult (male and female) participants with poor dietary habits |
| SAMEA111391305 | health_condition_detail | Healthy adult participants with poor dietary habits | R3 | 0.70 | paper.fulltext.methods | Healthy adult (male and female) participants with poor dietary habits |
| SAMEA111391305 | intervention | no_intervention | R1 | 0.85 | biosample.attribute:description | CONT |
| SAMEA111391305 | location_locality | Cork | R3 | 0.70 | paper.fulltext.methods | recruited from the Cork area |
| SAMEA111391305 | subject_id | 1 | R1 | 0.70 | sample.name_rule:alias | 1v2_S1_L001 |
Sequencing
380 runs over 380 samples · mean 0.35 Gbp per run (median 0.30 Gbp, total 132.38 Gbp; 380 runs with a base count) · layout PAIRED 380 · platform ILLUMINA 380 · models NextSeq 550 (380) · 82% of runs with a Sandpiper profile · first public 2022-09-23
Authors (12)
Berding K first · Bastiaanssen TFS · Moloney GM · Boscaini S · Strain CR · Anesi A · Long-Smith C · Mattivi F · Stanton C · Clarke G · Dinan TG · Cryan JF last
Downloads
PRJEB56264.csv.gz (8.5 KB) · parquet (82.1 KB) — all 380 samples, every field with route and confidence · PRJEB56264_determinations.csv.gz (16.8 KB) — 2,824 evidence rows