Gut microbiome after RYGB vs sleeve gastrectomy, T2D, Norway
154 fecal samples from adult patients with type 2 diabetes in Norway, randomized to gastric bypass or sleeve gastrectomy; triple-blind randomized controlled trial comparing microbiome changes after surgery. machine-written summary
154 samples · 154 runs · NO · Adult · 2026 · 5.06 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 1,077 sample values (collection date, country, latitude, location region, longitude, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 616 sample values (health condition, health condition detail, location locality, location site) · PMC13218940, PMC6561424 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Surgical procedure studied before/after (bariatric surgery design randomized_controlled_trial · population type2_diabetes · Gastric bypass (RYGB) vs sleeve gastrectomy (SG) for T2D
“randomized to either SG or RYGB” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal
77 of 77 people sampled more than once (154 samples; median 2, up to 2 per person) · follow-up median 12 mo (max 12 mo) · median interval between samples 12 mo (time from timepoint labels). Schedule stated by the source: “faecal samples before and 12 months after surgery” (time origin: surgery); stated follow-up 12 mo.
Each row is one person (36 shown of 77); dots are samples at days since that person's first sample.
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 154 | 100% | ||||
| country | 154 | 100% | 154 | |||
| health_condition | 154 | 100% | 154 | |||
| subject_id | 154 | 100% | 154 | |||
| collection_date | 154 | 100% | 154 | |||
| detailed_location | 154 | 100% | ||||
| health_condition_detail | 154 | 100% | 154 | |||
| latitude | 154 | 100% | 154 | |||
| location_locality | 154 | 100% | 154 | |||
| location_region | 154 | 100% | 154 | |||
| location_site | 154 | 100% | 154 | |||
| longitude | 154 | 100% | 154 | |||
| timepoint_label | 153 | 99% | 153 |
- Single-centre, triple-blinded, randomised, 1-year, parallel-group, superiority study to compare the effects of Roux-en-Y gastric bypass and sleeve gastrectomy on remission of type 2 diabetes and ß-cell function in subjects with morbid obesity: a protocol for the Obesity surgery in Tønsberg (Oseberg) study. 2019 PMID 31167860 PMC6561424 own_data
- Gastric bypass versus sleeve gastrectomy in patients with type 2 diabetes (Oseberg): a single-centre, triple-blind, randomised controlled trial. 2019 PMID 31678062 own_data
- Gut microbiota responses to bariatric surgery are associated with metabolic outcomes and type 2 diabetes remission. 2026 PMID 42098417 PMC13218940 own_data
Archive description
Bariatric surgery is the most efficient treatment for type 2 diabetes (T2D) and has been associated with changes in the gut microbiome. The altered gut microbiome observed after bariatric surgery is suggested to contribute to improved glucose metabolism in the host. Sleeve gastrectomy (SG) is the most common type of bariatric surgery, while gastric bypass (RYGB) has a higher frequency of T2D remission. Here we analyzed the fecal microbiome in patients with T2D randomized to either SG or RYGB in a triple-blind, randomized controlled trial. Both surgeries shifted the microbiome majorly in a similar direction, but RYGB produced larger shifts 1 year after surgery. The extent of microbiome change was associated with higher circulating GLP-1 levels and beta-cell function following surgery, independent of weight loss. The functional potential of the microbiota was also altered, along with shift in fermentation products, in both surgery types. We observed decreased potential for carbohydrate degradation in the post-bariatric surgery microbiome, with accompanying increase in potential for butyrate production, especially from amino acids such as lysine. Both surgeries were associated with increased gene richness and methanogenic Archaea, while RYGB was associated with increased relative abundance of Escherichia spp. following surgery. Both increased shift in fermentation products and higher increase in gene richness, and larger increase in potential for butyrate production from amino acids was linked to T2D remission. Increased abundance of Archaea and gene richness that previously been associated with metabolic health. These shifts indicates that the gut maintains an anaerobic environment following surgery and suggest that microbial adaptations to the altered milieu following surgery may contribute to metabolic improvements in the host.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.78. Assay shotgun_dna.
- host run.scientific_name · “Homo sapiens”
- body site study.description · “fecal microbiome in patients with T2D”
- life stage study.description · “patients with T2D randomized”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA112655990 | collection_date | 2017 | R1 | 0.90 | biosample.attribute:collection_date | 2017 |
| SAMEA112655990 | country | NO | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Norway |
| SAMEA112655990 | health_condition | type2_diabetes | R3 | 0.70 | paper.fulltext.abstract | individuals with obesity and type 2 diabetes |
| SAMEA112655990 | health_condition_detail | individuals with obesity and type 2 diabetes (T2D) | R3 | 0.70 | paper.fulltext.abstract | individuals with obesity and type 2 diabetes |
| SAMEA112655990 | latitude | 59.2675 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 59.26754 10.40762 |
| SAMEA112655990 | location_locality | Tønsberg | R3 | 0.70 | paper.fulltext.methods | Vestfold Hospital Trust (Tønsberg, Norway) |
| SAMEA112655990 | location_region | Vestfold | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 59.26754 10.40762 |
| SAMEA112655990 | location_site | Vestfold Hospital Trust | R3 | 0.70 | paper.fulltext.methods | conducted at Vestfold Hospital Trust (Tønsberg, Norway) |
| SAMEA112655990 | longitude | 10.4076 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 59.26754 10.40762 |
| SAMEA112655990 | subject_id | 2 | R1 | 0.70 | sample.name_rule:alias | 2_12M |
| SAMEA112655990 | timepoint_label | 12 months | R1 | 0.70 | sample.name_rule:alias | 2_12M |
Sequencing
154 runs over 154 samples · mean 5.23 Gbp per run (median 5.06 Gbp, total 805.95 Gbp; 154 runs with a base count) · layout PAIRED 154 · platform ILLUMINA 154 · models NextSeq 500 (154) · 0% of runs with a Sandpiper profile · first public 2026-03-03
Authors (32)
Borgeraas H first · Hjelmesæth J · Birkeland KI · Fatima F · Grimnes JO · Gulseth HL · Halvorsen E · Hertel JK · Hillestad TOW · Johnson LK · Karlsen TI · Kolotkin RL · Kvan NP · Lindberg M · Lorentzen J · Nordstrand N · Sandbu R · Seeberg KA · Seip B · Svanevik M · Valderhaug TG · Hofsø D last · Cvancarova Småstuen M · Stefanovski D · Gretland Valderhaug T · Olsson LM first · Chakaroun RM · Dwibedi C · Mitteregger M · Holst JJ · Tremaroli V · Bäckhed F last
Downloads
PRJEB59861.csv.gz (5.2 KB) · parquet (76.9 KB) — all 154 samples, every field with route and confidence · PRJEB59861_determinations.csv.gz (10.9 KB) — 1,693 evidence rows