Infant gut microbiota, HMOs and cognition, USA, longitudinal

While ample research on independent associations between infant cognition and gut microbiota composition and human milk (HM) oligosaccharides (HMOs) has been reported, studies on how the interactions between gut microbiota and HMOs may yield associations with cognitive development in infancy are lacking. We aimed to determine how HMOs and species of Bacteroides and Bifidobacterium genera interact with each other and their associations with cognitive development in typically developing infants. A total of 105 mother-infant dyads were included in this study. The enrolled infants (2.9-12 months old (8.09±2.48)) were at least predominantly breastfed at 4 months old. A total of 170 HM samples from the mothers and fecal samples of the children were collected longitudinally. Using the Mullen Scales of Early Learning to assess cognition and the scores as the outcomes, linear mixed effects models including both the levels of eight HMOs and relative abundance of Bacteroides and Bifidobacterium species as main associations and their interactions were employed with adjusting covariates infant sex, delivery mode, maternal education, site and batch effects of HMOs. Additionally, regression models stratifying infants based on the A-tetrasaccharide (A-tetra) status of the HM they received were also employed to determine if the associations depend on the A-tetra status. With Bacteroides species, we observed significant associations with motor functions, while Bif.catenulatum showed a negative association with visual reception in the detectable A-tetra group both as main effect (p-value=0.012) and in interaction with LNFP-I (p-value=0.007). Additionally, 3-FL showed a positive association with gross motor (p=0.027) and visual reception (p=0.041). Furthermore, significant associations were observed with the interaction terms mainly in the undetectable A-tetra group. Specifically, we observed negative associations for Bifidobacterium species and LNT (breve (p=0.011) and longum (p=0.022)), and positive associations for expressive language with 3’-SL and Bif.bifidum (p=0.01), 6’-SL and B.fragilis (p=0.019), and LNFP-I and Bif.kashiwanohense (p=0.048), respectively. Our findings suggest that gut microbiota and HMOs are both independently and interactively associated with early cognitive development. In particular, the diverse interactions between HMOs and Bacteroides and Bifidobacterium species reveal different candidate pathways through which HMOs, Bifidobacterium and Bacteroides species potentially interact to impact cognitive development in infancy.

Classified as human human milk / gut / stool shotgun DNA metagenome of infant / toddler and child and adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.

• host external_curation.infant_catalog · “infant triage verdict include”
• body site study.title · “and Bacteroides and Human Milk Oligosaccharides and Their”
• body site study.description · “microbiota composition and human milk (HM) oligosaccharides (HMOs)”
• body site study.description · “infant cognition and gut microbiota composition and”
• body site external_curation.infant_catalog · “infant triage verdict include (gut/stool scope)”
• life stage external_curation.infant_catalog · “infant triage verdict include (0-3 y scope)”
• life stage study.title · “Their Associations with Infant Cognition”
• life stage study.description · “samples of the children were collected longitudinally.”
• life stage study.description · “samples from the mothers and fecal samples”

170 runs over 170 samples · mean 5.06 Gbp per run (median 5.00 Gbp, total 859.98 Gbp; 170 runs with a base count) · layout PAIRED 170 · platform ILLUMINA 170 · models Illumina NovaSeq 6000 (170) · 89% of runs with a Sandpiper profile · first public 2023-06-22 – 2023-06-25

Cho S first · Samuel TM · Li T · Howell BR · Baluyot K · Hazlett HC · Elison JT · Zhu H · Hauser J · Sprenger N · Lin W last

3 of the 4 core fields are below 50% coverage: age_category, health_condition, subject_id. Share metadata

PRJEB63183.csv.gz (4.9 KB) · parquet (76.1 KB) — all 170 samples, every field with route and confidence · PRJEB63183_determinations.csv.gz (1.9 KB) — 340 evidence rows