RCT of Multi-Donor FMT for Chronic Pouchitis, Denmark
177 faecal samples from adult patients with chronic pouchitis and faecal donors, collected across five Danish hospitals; randomised placebo-controlled trial testing multi-donor FMT versus placebo. machine-written summary
177 samples · 179 runs · DK · Adult · 2024 · 2.51 Gbp / sample · cohort Fecal microbiota transplantation from a healthy po
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 480 sample values (collection date, country, health condition, health condition detail) |
| ○ Abstract / description | read; no usable statement |
| ○ Full text | read; no usable statement or table · PMC12118383 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Faecal microbiota transplantation design randomized_controlled_trial · population ibd_or_gi_disease · Non-pooled multi-donor FMT by enema for 4 weeks
“randomised to treatment with non-pooled multi-donor FMT” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal — not resolvedFMT donor → recipient — not resolved
Schedule stated by the source: “4-week FMT intervention (enema daily 2 weeks, then every 2nd day for 2 weeks); PDAI assessed before treatment and at 30-day follow-up; total follow-up 12 months.” (time origin: FMT); stated follow-up 12 mo.
FMT RCT for chronic pouchitis; patient (PaPr) and donor (DoBa) stool sequenced; donor->recipient mapping not in metadata.
Genera, mean relative abundance over 114 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 177 | 100% | ||||
| country | 177 | 100% | 177 | |||
| health_condition | 63 | 36% | 63 | |||
| subject_id | 0 | 0% | ||||
| collection_date | 177 | 100% | 177 | |||
| health_condition_detail | 63 | 36% | 63 |
- Fecal microbiota transplantation from a healthy pouch donor for chronic pouchitis: a proof-of-concept study. 2025 PMID 40413728 PMC12118383 unsure
Archive description
Objective: To investigate if treatment with non-pooled multi-donor faecal microbiota transplantation (FMT) for four weeks was superior to placebo for inducing clinical remission in patients with chronic pouchitis. Design: The study was a randomised placebo-controlled study with a 4-week intervention period and 12 months follow-up. Eligible patients with chronic pouchitis were recruited from five Danish hospitals. Patients were randomised to treatment with non-pooled multi-donor FMT derived from four faecal donors, or placebo. The study treatment was delivered by enema daily for two weeks followed by every second day for two weeks. Disease severity was accessed before and after treatment at 30-day follow-up, using the Pouchitis Disease Activity Index (PDAI). A PDAI <7 was considered equivalent to clinical remission. The gut microbiota in faecal samples from patients and donors was analysed using shotgun metagenomic sequencing.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Placebo-Controlled Trial of Faecal Microbiota Transplantation in”
- body site study.description · “with non-pooled multi-donor faecal microbiota transplantation (FMT)”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA114515858 | collection_date | 2019-09-03 | R1 | 0.90 | biosample.attribute:collection_date | 2019-09-03 |
| SAMEA114515858 | country | DK | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Denmark |
| SAMEA114515926 | health_condition | other_disease | R1 | 0.85 | biosample.attribute:description | description: Illumina shotgun metagenome of human fecal microbiome from patient |
| SAMEA114515926 | health_condition_detail | Illumina shotgun metagenome of human fecal microbiome from patient | R1 | 0.85 | biosample.attribute:description | description: Illumina shotgun metagenome of human fecal microbiome from patient |
Sequencing
179 runs over 177 samples · mean 2.31 Gbp per run (median 2.51 Gbp, total 413.94 Gbp; 179 runs with a base count) · layout PAIRED 179 · platform ILLUMINA 179 · models Illumina NovaSeq 6000 (179) · 64% of runs with a Sandpiper profile · first public 2024-05-09 – 2026-04-09
Authors (5)
Kousgaard SJ first · Dall SM · Albertsen M · Nielsen HL · Thorlacius-Ussing O last
Help complete this project
2 of the 4 core fields are below 50% coverage: health_condition, subject_id. Share metadata
Downloads
PRJEB66493.csv.gz (5.1 KB) · parquet (76.6 KB) — all 177 samples, every field with route and confidence · PRJEB66493_determinations.csv.gz (3.8 KB) — 480 evidence rows