Gut metagenomes in metastatic CRC trial, Italy
163 stool metagenome samples from adult metastatic colorectal cancer patients in Italy, collected in the AtezoTRIBE phase-II randomized trial comparing atezolizumab plus folfoxiri/bevacizumab to folfoxiri/bevacizumab alone. machine-written summary
163 samples · 163 runs · IT · Adult · 2025 · 4.28 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 434 sample values (collection date, country, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ○ Full text | read; no usable statement or table · PMC12283368 |
| ✓ Supplementary tables | 815 sample values (age, bmi, health condition, health condition detail, sex) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Non-antibiotic pharmaceutical given as the study intervention (metformin design randomized_controlled_trial · population colorectal_cancer · Atezolizumab added to FOLFOXIRI plus bevacizumab in mCRC
“impact of the atezolizumab immune therapy treatment to folfoxiri plus bevacizumab treatment” — bioproject.description (study level; per-sample arms are in the intervention column below)
Genera, mean relative abundance over 143 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 163 | 100% | ||||
| country | 163 | 100% | 163 | |||
| health_condition | 163 | 100% | 163 | |||
| subject_id | 0 | 0% | ||||
| age_at_collection_days | 163 | 100% | 163 | |||
| bmi | 163 | 100% | 163 | |||
| collection_date | 163 | 100% | 163 | |||
| health_condition_detail | 163 | 100% | 163 | |||
| sex | 163 | 100% | 163 | |||
| timepoint_label | 108 | 66% | 108 |
- Pooled analysis of 3,741 stool metagenomes from 18 cohorts for cross-stage and strain-level reproducible microbial biomarkers of colorectal cancer. 2025 PMID 40461820 PMC12283368 unsure
Archive description
Shotgun metagenomic stool samples for the AtezoTRIBE phase-II randomized trial which was established to study the impact of the atezolizumab immune therapy treatment to folfoxiri plus bevacizumab treatment in unresectable metastatic colorectal cancer (CRC) patients.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Gut microbiome samples from”
- body site study.description · “Shotgun metagenomic stool samples for the”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA115284616 | age_at_collection_days | 24837.0 | R2 | 0.85 | paper.supp.41591_2025_3693_MOESM2_ESM.xlsx[Table 2][Age] | 68 |
| SAMEA115284616 | bmi | 19.15 | R2 | 0.85 | paper.supp.41591_2025_3693_MOESM2_ESM.xlsx[Table 2][BMI] | 19.1511918197052 |
| SAMEA115284616 | collection_date | 2019 | R1 | 0.90 | biosample.attribute:collection_date | 2019 |
| SAMEA115284616 | country | IT | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Italy |
| SAMEA115284616 | health_condition | colorectal_cancer | R2 | 0.85 | paper.supp.41591_2025_3693_MOESM2_ESM.xlsx[Table 2][Study condition] | CRC |
| SAMEA115284616 | health_condition_detail | CRC | R2 | 0.85 | paper.supp.41591_2025_3693_MOESM2_ESM.xlsx[Table 2][Study condition] | CRC |
| SAMEA115284616 | sex | male | R2 | 0.85 | paper.supp.41591_2025_3693_MOESM2_ESM.xlsx[Table 2][Sex] | male |
| SAMEA115284617 | timepoint_label | V1 | R1 | 0.70 | biosample.name_rule:title | ATZ_142_V1 |
Sequencing
163 runs over 163 samples · mean 5.81 Gbp per run (median 4.28 Gbp, total 946.90 Gbp; 163 runs with a base count) · layout PAIRED 163 · platform ILLUMINA 163 · models unspecified (163) · 88% of runs with a Sandpiper profile · first public 2025-01-15 – 2025-03-17
Authors (40)
Piccinno G first · Thompson KN · Manghi P · Ghazi AR · Thomas AM · Blanco-Míguez A · Asnicar F · Mladenovic K · Pinto F · Armanini F · Punčochář M · Piperni E · Heidrich V · Fackelmann G · Ferrero G · Tarallo S · Nguyen LH · Yan Y · Keles NA · Tuna BG · Vymetalkova V · Trompetto M · Liska V · Hucl T · Vodicka P · Bencsiková B · Čarnogurská M · Popovici V · Marmorino F · Cremolini C · Pardini B · Cordero F · Song M · Chan AT · Derosa L · Zitvogel L · Huttenhower C · Naccarati A · Budinska E · Segata N last
Downloads
PRJEB72523.csv.gz (5.7 KB) · parquet (77.2 KB) — all 163 samples, every field with route and confidence · PRJEB72523_determinations.csv.gz (11.1 KB) — 1,249 evidence rows