Prebiotic trial in older UK twins for muscle and cognition
137 gut metagenome samples from healthy older adults (≥60) in the United Kingdom, from a placebo-controlled, double-blinded, randomised twin-pair trial testing a prebiotic plus exercise and BCAA supplementation on muscle function and cognition. machine-written summary
137 samples · 137 runs · GB · Elderly · 2024 · 6.11 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 959 sample values (collection date, country, latitude, location region, longitude, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 274 sample values (health condition, health condition detail) · PMC10904794 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| ✓ External resources | 137 sample values (antibiotic exposure) · from BugSigDB |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Non-microbial dietary supplement (vitamins Exercise / physical-training programme Prebiotic / dietary fibre supplement design randomized_controlled_trial · population healthy_control · Prebiotic vs placebo 12 weeks, with resistance exercise and BCAA supplementation for all
“randomised to receive either placebo or prebiotic daily” — abstract:38424099 (study level; per-sample arms are in the intervention column below)
longitudinal — not resolvedtwins — not resolved
65 of 72 people sampled more than once (130 samples; median 2, up to 2 per person). Schedule stated by the source: “Two visits per participant: V1 (baseline) and V2 (end of 12-week prebiotic/placebo intervention)”.
36 twin pairs (72 individuals, monozygotic/dizygotic), one twin per pair to each study arm.
Genera, mean relative abundance over 128 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 137 | 100% | ||||
| country | 137 | 100% | 137 | |||
| health_condition | 137 | 100% | 137 | |||
| subject_id | 137 | 100% | 137 | |||
| antibiotic_exposure | 137 | 100% | 137 | |||
| collection_date | 137 | 100% | 137 | |||
| detailed_location | 137 | 100% | ||||
| health_condition_detail | 137 | 100% | 137 | |||
| latitude | 137 | 100% | 137 | |||
| location_region | 137 | 100% | 137 | |||
| longitude | 137 | 100% | 137 | |||
| timepoint_label | 137 | 100% | 137 |
- Effect of gut microbiome modulation on muscle function and cognition: the PROMOTe randomised controlled trial. 2024 PMID 38424099 PMC10904794 own_data
Archive description
Studies suggest that inducing gut microbiota changes may alter both muscle physiology and cognitive behaviour. Gut microbiota may play a role in both anabolic resistance of older muscle, and cognition. In this placebo controlled double blinded randomised controlled trial of 36 twin pairs (72 individuals), aged >= 60,each twin pair are block randomised to receive either placebo or prebiotic daily for 12 weeks. Resistance exercise and branched chain amino acid (BCAA) supplementation is prescribed to all participants. Outcomes are physical function and cognition. The trial is carried out remotely using video visits, online questionnaires and cognitive testing, and posting of equipment and biological samples. The prebiotic supplement is well tolerated and results in a changed gut microbiome [e.g., increased relative Bifidobacterium abundance]. There is no significant difference between prebiotic and placebo for the primary outcome of chair rise time (β=0.579 95% CI -1.080-2.239 p=0.494). The prebiotic improves cognition (factor score versus placebo (β=-0.482 95% CI, -0.813, -0.141 p=0.014)). Our results demonstrate that cheap and readily available gut microbiome interventions may improve cognition in our ageing population. We illustrate the feasibility of remotely delivered trials for older people, which could reduce under-representation of older people in clinical trials. ClinicalTrials.gov registration: NCT04309292.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of elderly by sonnet_x2 with confidence 0.75. Assay shotgun_dna.
- host run.scientific_name · “Homo sapiens”
- body site study.description · “Gut microbiota may play a role”
- life stage study.description · “aged >= 60”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA115368570 | antibiotic_exposure | no | R2 | 0.60 | external.bugsigdb source | Antibiotics exclusion: 3 months |
| SAMEA115368570 | collection_date | 2021 | R1 | 0.90 | biosample.attribute:collection_date | 2021 |
| SAMEA115368570 | country | GB | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | United Kingdom |
| SAMEA115368570 | health_condition | healthy_control | R3 | 0.70 | paper.fulltext.abstract | randomised to receive either placebo or prebiotic |
| SAMEA115368570 | health_condition_detail | prebiotic vs placebo RCT with BCAA and exercise in older twins | R3 | 0.70 | paper.fulltext.abstract | randomised to receive either placebo or prebiotic |
| SAMEA115368570 | latitude | 51.4980 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 51.49802 -0.11088 |
| SAMEA115368570 | location_region | England | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 51.49802 -0.11088 |
| SAMEA115368570 | longitude | -0.1109 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 51.49802 -0.11088 |
| SAMEA115368570 | subject_id | F8_T1 | R1 | 0.70 | sample.name_rule:alias | F8_T1_V1 |
| SAMEA115368570 | timepoint_label | V1 | R1 | 0.70 | sample.name_rule:alias | F8_T1_V1 |
Sequencing
137 runs over 137 samples · mean 6.43 Gbp per run (median 6.11 Gbp, total 880.60 Gbp; 137 runs with a base count) · layout PAIRED 137 · platform ILLUMINA 137 · models Illumina HiSeq 2000 (137) · 93% of runs with a Sandpiper profile · first public 2024-03-01
Authors (18)
Ni Lochlainn M first · Bowyer RCE · Moll JM · García MP · Wadge S · Baleanu AF · Nessa A · Sheedy A · Akdag G · Hart D · Raffaele G · Seed PT · Murphy C · Harridge SDR · Welch AA · Greig C · Whelan K · Steves CJ last
Downloads
PRJEB72531.csv.gz (4.4 KB) · parquet (75.7 KB) — all 137 samples, every field with route and confidence · PRJEB72531_determinations.csv.gz (10.7 KB) — 1,370 evidence rows