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PRJEB7369 ERP007090

Gut microbiome of Swedish students before/after travel

Influence of travel on antibiotic resistance

70 fecal samples from healthy Swedish university students, collected before and after travel to the Indian peninsula or Central Africa, in a longitudinal study of antibiotic resistance genes and Blastocystis carriage. machine-written summary

70 samples · 70 runs · SE · Adult · 2015 · 18.52 Gbp / sample

Sources
✓Sequence archive 350○Abstract / description○Full text○Supplementary tables✓External resources 350–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive350 sample values (collection date, country, latitude, location region, longitude)
○ Abstract / descriptionread; no usable statement
○ Full textread; no usable statement or table · PMC10688793, PMC10756304, PMC12390848, PMC13106387, PMC13269251, PMC4576037, PMC5725903, PMC8288446
○ Supplementary tablesfiles read; no table could be matched to the samples
✓ External resources350 sample values (age, antibiotic exposure, health condition, health condition detail, sex) · from curatedMetagenomicData 3 (cMD 3)
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
adult70
Condition
healthy_control70
Sex
female52
male18
Country
SE70
Body site
primary70
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)all projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 3 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 64 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)this project, 30–100 Gbp: 3 samplesall projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 18.52 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

Schedule stated by the source: “Each of 35 subjects sampled twice: once before and once after a student exchange trip to the Indian peninsula or Central Africa.”.

How longitudinal and relatedness information is derived

Community composition
Bacteroides 12.2%Phocaeicola 10.9%Prevotella 9.1%Roseburia 7.6%Faecalibacterium 4.9%Alistipes 4.7%Lachnospira 2.4%Acetatifactor 2.4%Parabacteroides 1.6%Ruminococcoides 1.5%Vescimonas 1.2%Hominimerdicola 1.1%not assigned to a genus 3.3%other genera 37.3%

Genera, mean relative abundance over 68 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category70 100%
country70 100%70
health_condition70 100%70
subject_id0 0%
age_at_collection_days70 100%70
antibiotic_exposure70 100%70
collection_date70 100%70
detailed_location70 100%
health_condition_detail70 100%70
latitude70 100%70
location_region70 100%70
longitude70 100%70
sex70 100%70
Papers (8)
  • The Human Gut Microbiome as a Transporter of Antibiotic Resistance Genes between Continents. 2015 PMID 26259788 PMC4576037 own_data
  • The relation between Blastocystis and the intestinal microbiota in Swedish travellers. 2017 PMID 29228901 PMC5725903 own_data
  • Dispersal strategies shape persistence and evolution of human gut bacteria. 2021 PMID 34111423 PMC8288446 unsure
  • Profiling of the intestinal community of Clostridia: taxonomy and evolutionary analysis. 2023 PMID 38047279 PMC10688793 unsure
  • Identification of a muropeptide precursor transporter from gut microbiota and its role in preventing intestinal inflammation. 2023 PMID 38127978 PMC10756304 unsure
3 more
Details
Archive description

We used untargeted metagenomic Illumina sequencing to study fecal samples of 35 Swedish healthcare students, taken before and after embarking on a student exchange program to the Indian peninsula or central Africa. The students were not taking antibiotics between sampling points. Antibiotic resistance gene frequencies were investigated in all samples.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.82. Assay shotgun_dna.

  • host run.scientific_name · “human gut metagenome”
  • body site study.description · “fecal samples of 35 Swedish”
  • life stage study.description · “Swedish healthcare students”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMEA2784448age_at_collection_days9131.25R20.75external.cmd sourceage: 25; age_unit: Year; age_group: Adult
SAMEA2784448antibiotic_exposurenoR20.75external.cmd sourceantibiotics_current_use: No
SAMEA2784448collection_date2013-05-23R10.90biosample.attribute:collection_date2013-05-23
SAMEA2784448countrySER10.90sample.attr.geographic_location_country_and_or_seaSweden
SAMEA2784448health_conditionhealthy_controlR20.75external.cmd sourcedisease: Healthy
SAMEA2784448health_condition_detailHealthyR20.75external.cmd sourcedisease: Healthy
SAMEA2784448latitude63.8186R10.90biosample.attribute:geographic_location_latitude+geographic_location_longitude63.818577 20.296611
SAMEA2784448location_regionVaesterbottenR10.60biosample.attribute:geographic_location_latitude+geographic_location_longitude63.818577 20.296611
SAMEA2784448longitude20.2966R10.90biosample.attribute:geographic_location_latitude+geographic_location_longitude63.818577 20.296611
SAMEA2784448sexfemaleR20.75external.cmd sourcesex: Female
Sequencing

70 runs over 70 samples · mean 18.62 Gbp per run (median 18.52 Gbp, total 1,303.15 Gbp; 70 runs with a base count) · layout PAIRED 70 · platform ILLUMINA 70 · models Illumina HiSeq 2000 (70) · 97% of runs with a Sandpiper profile · first public 2015-06-01

Authors (66)

Forsell J first · Bengtsson-Palme J · Angelin M · Johansson A · Evengård B · Granlund M last · Candeliere F first · Musmeci E · Amaretti A · Sola L · Raimondi S · Rossi M last · Lin NY first · Fukuoka S · Koyama S · Motooka D · Tourlousse DM · Shigeno Y · Matsumoto Y · Yamano H · Murotomi K · Tamaki H · Irie T · Sugiyama E · Kumagai S · Itahashi K · Tanegashima T · Fujimaki K · Ito S · Shindo M · Tsuji T · Wake H · Watanabe K · Maeda Y · Enokida T · Tahara M · Yamashita R · Fujisawa T · Nomura M · Kawazoe A · Goto K · Doi T · Shitara K · Mano H · Sekiguchi Y · Nakamura S · Benno Y · Nishikawa H last · Busi E · Heidrich V first · Fackelmann G · Ricci L · Spadazzi R · Baldanzi G · Punčochář M · Catassi G · Marchi P · Modesto M · Piccinno G · Porcari S · Rondinella D · Asnicar F · Valles-Colomer M · Mattarelli P · Ianiro G · Segata N last

Downloads

PRJEB7369.csv.gz (2.8 KB) · parquet (73.4 KB) — all 70 samples, every field with route and confidence · PRJEB7369_determinations.csv.gz (6.2 KB) — 700 evidence rows

Samples (70 · every field this project has values for · hover a cell for its source)
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