Gut microbiome in long-term fasting, Germany
Single-arm long-term fasting study (~9.8 days, ~250 kcal/day) in Germany: shotgun metagenomics of 241 stool samples from about 92 participants collected before, after and at follow-up of fasting. machine-written summary
241 samples · 241 runs · DE · Age unknown · 2025 · 3.43 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 1,202 sample values (collection date, country, intervention, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ○ Full text | read; no usable statement or table · PMC13227866, PMC13587497 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
88 people have samples in more than one arm.
design single_arm_before_after
longitudinal — not resolved
89 of 91 people sampled more than once (238 samples; median 2, up to 4 per person). Schedule stated by the source: “stool before fasting, after fasting, and at two follow-up visits (FUP_1, FUP_2)” (time origin: intervention_start).
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 0 | 0% | ||||
| country | 241 | 100% | 241 | |||
| health_condition | 0 | 0% | ||||
| subject_id | 240 | 100% | 240 | |||
| collection_date | 241 | 100% | 241 | |||
| intervention | 240 | 100% | 240 | |||
| timepoint_label | 240 | 100% | 240 |
- Long-term fasting remodels gut microbial metabolism and host metabolism 2024 own_data
Archive description
To be added
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of age unknown by curator_audit with confidence 0.50. Assay shotgun_dna.
- host runs.scientific_name · “human gut metagenome (241)”
- host runs.environment_material · “feces (241)”
- body site runs.scientific_name · “human gut metagenome (241)”
- body site runs.environment_material · “feces (241)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA115423614 | collection_date | 2022 | R1 | 0.85 | biosample.attribute:collection date | collection date: 2022 |
| SAMEA115423614 | country | DE | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Germany |
| SAMEA115423614 | intervention | no_intervention | R1 | 0.70 | sample.name_rule:DESCRIPTION+preprint.abstract | DESCRIPTION: GEN_2_Before_Stool |
| SAMEA115423614 | subject_id | GEN_2 | R1 | 0.70 | sample.name_rule:DESCRIPTION | DESCRIPTION: GEN_2_Before_Stool |
| SAMEA115423614 | timepoint_label | Before | R1 | 0.70 | sample.name_rule:DESCRIPTION | DESCRIPTION: GEN_2_Before_Stool |
Sequencing
241 runs over 241 samples · mean 3.46 Gbp per run (median 3.43 Gbp, total 834.47 Gbp; 241 runs with a base count) · layout PAIRED 241 · platform ILLUMINA 241 · models Illumina NovaSeq 6000 (241) · 92% of runs with a Sandpiper profile · first public 2025-01-20
Authors (0)
No author record.
Help complete this project
2 of the 4 core fields are below 50% coverage: age_category, health_condition. Share metadata
Downloads
PRJEB74035.csv.gz (6.8 KB) · parquet (77.8 KB) — all 241 samples, every field with route and confidence · PRJEB74035_determinations.csv.gz (12.0 KB) — 1,202 evidence rows