Gut microbiome in a modified fasting trial, healthy adults, Germany
Shotgun metagenomic sequencing of 185 longitudinal stool samples from 62 healthy adult subjects (Germany) in a randomised controlled trial comparing a 5-day hypocaloric ketogenic modified fasting program to no intervention. machine-written summary
185 samples · 189 runs · DE · Adult · 2024 · 3.42 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 740 sample values (collection date, country, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 432 sample values (antibiotic exposure, health condition, health condition detail) · PMC13227866 |
| ✓ Supplementary tables | 185 sample values (intervention) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
Controlled / prescribed diet or feeding regimen design randomized_controlled_trial · population healthy_control · 5-day hypocaloric (~600 kcal/day) ketogenic modified fasting program
“randomised to MFP or control group” — abstract:42226305 (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
62 of 62 people sampled more than once (185 samples; median 3, up to 3 per person). Schedule stated by the source: “baseline (pre), end of 5-day MFP (post), after food reintroduction, one month follow-up (fup)”.
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 185 | 100% | ||||
| country | 185 | 100% | 185 | |||
| health_condition | 185 | 100% | 185 | |||
| subject_id | 185 | 100% | 185 | |||
| collection_date | 185 | 100% | 185 | |||
| health_condition_detail | 185 | 100% | 185 | |||
| intervention | 185 | 100% | 185 | |||
| timepoint_label | 185 | 100% | 185 | |||
| antibiotic_exposure | 62 | 34% | 62 |
- Health benefits of a five-day at-home modified fasting program: a randomised controlled trial 2026 PMID 42226305 PMC13227866 own_data
Archive description
To be completed
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by curator_audit with confidence 0.53. Assay shotgun_dna.
- host runs.scientific_name · “human gut metagenome (189)”
- host papers[0].abstract · “gut metagenomics analyses”
- body site runs.scientific_name · “human gut metagenome (189)”
- body site papers[0].abstract · “gut metagenomics analyses”
- life stage study.title · “risk factors for cardiometabolic disease”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA115529281 | antibiotic_exposure | no | R3 | 0.70 | paper.body.PMC13227866[methods: exclusion criteria] | intake of antibiotics up to 2 months prior the study |
| SAMEA115529281 | collection_date | 2023 | R1 | 0.85 | biosample.attribute:collection date | collection date: 2023 |
| SAMEA115529281 | country | DE | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Germany |
| SAMEA115529281 | health_condition | healthy_control | R3 | 0.70 | paper.body.PMC13227866 | sixty-four healthy subjects were randomised to MFP or control group |
| SAMEA115529281 | health_condition_detail | healthy subjects | R3 | 0.70 | paper.body.PMC13227866 | sixty-four healthy subjects were randomised to MFP or control group |
| SAMEA115529281 | intervention | diet_intervention | R2 | 0.85 | paper.supp.13073_2026_1681_MOESM1_ESM.xlsx[S33!group] | S9448Nr1: group=MFP |
| SAMEA115529281 | subject_id | 1 | R1 | 0.70 | sample.name_rule:library_names | Participant 1 pre |
| SAMEA115529281 | timepoint_label | pre | R1 | 0.70 | sample.name_rule:library_names | Participant 1 pre |
Sequencing
189 runs over 185 samples · mean 3.50 Gbp per run (median 3.42 Gbp, total 662.21 Gbp; 189 runs with a base count) · layout PAIRED 189 · platform ILLUMINA 189 · models Illumina NovaSeq 6000 (189) · 97% of runs with a Sandpiper profile · first public 2024-12-04
Authors (0)
No author record.
Downloads
PRJEB75353.csv.gz (5.6 KB) · parquet (76.4 KB) — all 185 samples, every field with route and confidence · PRJEB75353_determinations.csv.gz (11.0 KB) — 1,357 evidence rows