Microbiome across colorectal adenoma-carcinoma sequence
156 stool samples from Austria in a case-control study of healthy controls and patients with advanced colorectal adenoma or carcinoma, tracking gut microbiome changes along the adenoma-carcinoma sequence. machine-written summary
156 samples · 312 runs · AT · Adult · 2015 · 5.00 Gbp / sample · cohort Contributions of Human-Associated Archaeal Metabol
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 1,060 sample values (collection date, country, health condition, health condition detail, latitude, location region, longitude) |
| ○ Abstract / description | read; no usable statement |
| ○ Full text | read; no usable statement or table · PMC10457972, PMC12035788, PMC12539022, PMC13184533, PMC13281578, PMC7283552, PMC8851854 |
| ✓ Supplementary tables | 329 sample values (age, bmi, sex, subject id) |
| ✓ External resources | 402 sample values (age, antibiotic exposure, bmi, sex) · from curatedMetagenomicData 3 (cMD 3) |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
health condition: sequence archive and curatedMetagenomicData disagree for 32 samples (e.g. healthy_control vs cardiometabolic_other) — withheld.
The sources disagree on 32 samples. Where two incompatible values exist for one sample (or one person's samples disagree on sex), neither value is published: the field is left empty here, in the sample sheet and in the downloads. Both sides, with their sources, are in gut_metadata_contradictions.parquet of the data package (what counts as a contradiction).
Contradicted values (32)
| sample | field | value | other value | handling |
|---|---|---|---|---|
| SAMEA3136623 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136624 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136626 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136627 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136628 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136629 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136630 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136634 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136635 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136636 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136640 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136643 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136644 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136648 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136649 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136652 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136653 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136657 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136658 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136659 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136661 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136662 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136663 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136664 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136668 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136670 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136675 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136677 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136678 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3136679 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3178937 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
| SAMEA3178938 | health condition | healthy_control (sequence archive) | cardiometabolic_other (curatedMetagenomicData) | withheld: neither value published |
Genera, mean relative abundance over 142 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 156 | 100% | ||||
| country | 156 | 100% | 156 | |||
| health_condition | 124 | 79% | 124 | |||
| subject_id | 109 | 70% | 109 | |||
| age_at_collection_days | 156 | 100% | 156 | |||
| bmi | 156 | 100% | 156 | |||
| collection_date | 156 | 100% | 156 | |||
| detailed_location | 156 | 100% | ||||
| health_condition_detail | 156 | 100% | 156 | |||
| latitude | 156 | 100% | 156 | |||
| location_region | 156 | 100% | 156 | |||
| longitude | 156 | 100% | 156 | |||
| sex | 156 | 100% | 156 | |||
| antibiotic_exposure | 154 | 99% | 154 |
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Archive description
Colorectal cancer, a commonly diagnosed cancer in the elderly, often develops slowly from benign polyps called adenoma. The gut microbiota, with its critical roles in nutrient metabolism and immune modulation, is believed to be directly involved in colorectal carcinogenesis. The identity and functional capacity of the adenoma- or carcinoma-related gut microbe(s), however, have not been surveyed in a comprehensive manner. Here we perform metagenomewide association study (MGWAS) on stools from healthy controls, advanced adenoma and carcinoma patients, revealing gene markers from over 100 bacterial strains enriched in each group.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult and elderly by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Gut microbiome development along”
- body site study.description · “Colorectal cancer, a commonly”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- life stage study.description · “cancer in the elderly , often develops”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA3136623 | age_at_collection_days | 26663 | R2 | 0.85 | paper.supp.13099_2025_712_MOESM7_ESM.xlsx[Sheet1!Age (yrs)] | 73 |
| SAMEA3136680 | age_at_collection_days | 24837.0 | R2 | 0.75 | external.cmd source | age: 68; age_unit: Year; age_group: Elderly |
| SAMEA3136623 | antibiotic_exposure | no | R2 | 0.75 | external.cmd source | antibiotics_current_use: No |
| SAMEA3136623 | bmi | 32.0 | R2 | 0.85 | paper.supp.13099_2025_712_MOESM7_ESM.xlsx[Sheet1!BMI] | 32 |
| SAMEA3136680 | bmi | 34.01 | R2 | 0.75 | external.cmd source | bmi: 34.01 |
| SAMEA3136623 | collection_date | 2013 | R1 | 0.90 | biosample.attribute:collection_date | 2013 |
| SAMEA3136623 | country | AT | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Austria |
| SAMEA3136625 | health_condition | healthy_control | R1 | 0.85 | biosample.attribute:title | Stool sample from controls |
| SAMEA3136623 | health_condition_detail | Stool sample from controls | R1 | 0.85 | biosample.attribute:title | Stool sample from controls |
| SAMEA3136623 | latitude | 47.8015 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 47.80151 13.04292 |
| SAMEA3136623 | location_region | Salzburg | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 47.80151 13.04292 |
| SAMEA3136623 | longitude | 13.0429 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 47.80151 13.04292 |
| SAMEA3136623 | sex | female | R2 | 0.75 | external.cmd source | sex: Female |
| SAMEA3136625 | sex | female | R2 | 0.85 | paper.supp.13099_2025_712_MOESM7_ESM.xlsx[Sheet1!Gender (1:male, 2:female)] | ERR688409: 2 |
| SAMEA3136623 | subject_id | 31766 | R2 | 0.85 | paper.supp.13099_2025_712_MOESM8_ESM.xlsx[Sheet1!Sample Id] | ERR688419: 31766 |
Sequencing
312 runs over 156 samples · mean 2.50 Gbp per run (median 1.57 Gbp, total 778.91 Gbp; 312 runs with a base count) · layout PAIRED 156, SINGLE 156 · platform ILLUMINA 312 · models Illumina HiSeq 2000 (312) · 46% of runs with a Sandpiper profile · first public 2015-03-05
Authors (79)
Tarracchini C first · Fontana F · Mancabelli L · Lugli GA · Alessandri G · Turroni F · Ventura M · Milani C last · Orihara K first · Yahagi K · Saito Y · Watanabe Y · Sasai T · Hara T · Tsukuda N · Oki K · Fujimoto J · Matsuki T last · Ormaasen I first · Rudi K · Diep DB · Snipen L last · De Biase R · Bocchio F · Viappiani A · De Conto F · Nouvenne A · Ticinesi A · Bussolati O · Meschi T · Cecchi R · van Sinderen D · Haque S first · Bantun F · Jalal NA · Faidah H · Babalghith AO · Alobaidy MA · Aldairi AF · Ahmad F last · Dong Y first · Wang M · Zhou X · Wang P · Yan K · Wang S · Zhong JC · Li H · Zhao L · Li B · Li J last · Schroer HW first · Beghini F · Raygoza Garay JA · Christakis NA · Bosch DE last · Zhang Z first · Lu T · Dong B · Liu J · Zhang Y · Li S · Liu H · Li X · Guan T · Guo H · Yan Q · Lei Z · Yu X · Wang L · Kang J · Li L · Zhao D last · Weilguny L first · Probul N · Ren Y · Pons N · Baumbach J · Almeida M last
Downloads
PRJEB7774.csv.gz (6.1 KB) · parquet (78.6 KB) — all 156 samples, every field with route and confidence · PRJEB7774_determinations.csv.gz (18.4 KB) — 1,791 evidence rows