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PRJEB79382 ERP163565

Infant stool microbiota stability in home freezers

Stool microbiota stability

77 stool samples from Switzerland examining infant stool microbiota stability under domestic freezer storage conditions. machine-written summary

77 samples · 77 runs · CH · Infant / toddler · 2024 · 1.60 Gbp / sample

Sources
✓Sequence archive 231○Abstract / description○Full text○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive231 sample values (collection date, country, subject id)
○ Abstract / descriptionread; no usable statement
○ Full textread; no usable statement or table · PMC11878046
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet · share metadata
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
unknown77
Country
CH77
Body site
unknown77
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 3 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 3 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 70 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 1 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 1.60 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal — not resolved

20 of 20 people sampled more than once (77 samples; median 4, up to 4 per person). Schedule stated by the source: “none”.

How longitudinal and relatedness information is derived

Community composition
Bifidobacterium 15.8%Blautia_A 7.2%Bacteroides 6.7%Phocaeicola 4.9%Faecalibacterium 4.1%Roseburia 3.6%Klebsiella 3.5%Mediterraneibacter 2.9%Anaerostipes 2.9%Lactobacillus 2.1%Ruminococcoides 2.1%Collinsella 2.0%not assigned to a genus 5.7%other genera 36.5%

Genera, mean relative abundance over 77 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country77 100%77
health_condition0 0%
subject_id77 100%77
collection_date77 100%77
Papers (2)
  • Evaluating stool microbiome integrity after domestic freezer storage using whole-metagenome sequencing, genome assembly, and antimicrobial resistance gene analysis. PMID 39932403 PMC11878046 curated
  • Domestic freezer storage: a solution for preserving stool microbiota integrity for at-home collection curated
Details
Archive description

Infant stool microbiota stability in domestic freezers

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of infant / toddler by sonnet_x2 with confidence 0.55. Assay shotgun_dna.

  • host run.scientific_name · “human metagenome”
  • body site study.description · “Infant stool microbiota stability”
  • life stage study.description · “Infant stool microbiota stability”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMEA115957666collection_date2023-01-08R10.85biosample.attribute:collection_datecollection_date: 2023-01-08
SAMEA115957666countryCHR10.90biosample_attrSwitzerland
SAMEA115957666subject_id01R10.70sample.name_rule:ena.read_run.experiment_titleNextSeq 550 sequencing: Child01_month2
Sequencing

77 runs over 77 samples · mean 1.68 Gbp per run (median 1.60 Gbp, total 129.59 Gbp; 77 runs with a base count) · layout PAIRED 77 · platform ILLUMINA 77 · models NextSeq 550 (77) · 100% of runs with a Sandpiper profile · first public 2024-12-12

Authors (3)

Momo Cabrera P first · Bokulich NA · Zimmermann P last

Help complete this project

2 of the 4 core fields are below 50% coverage: age_category, health_condition. Share metadata

Downloads

PRJEB79382.csv.gz (2.7 KB) · parquet (72.2 KB) — all 77 samples, every field with route and confidence · PRJEB79382_determinations.csv.gz (2.3 KB) — 231 evidence rows

Samples (77 · every field this project has values for · hover a cell for its source)
Loading the sample table…