Fibromyalgia and gut microbiome case-control study in Latvia
41 gut microbiome samples from adults in Latvia, comparing 17 fibromyalgia patients to 24 healthy controls in a case-control design examining gut microbial diversity differences. machine-written summary
41 samples · 41 runs · LV · Adult · 2024 · 4.88 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 205 sample values (collection date, country, health condition, health condition detail, sex) |
| ✓ Abstract / description | 41 sample values (subject id) |
| ○ Full text | read; no usable statement or table · PMC11506677 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| ✓ External resources | 18 sample values (antibiotic exposure) · from GMrepo v3 |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 41 | 100% | ||||
| country | 41 | 100% | 41 | |||
| health_condition | 41 | 100% | 41 | |||
| subject_id | 41 | 100% | 41 | |||
| collection_date | 41 | 100% | 41 | |||
| health_condition_detail | 41 | 100% | 41 | |||
| sex | 41 | 100% | 41 | |||
| antibiotic_exposure | 18 | 44% | 18 |
- Human Herpesvirus-6B Infection and Alterations of Gut Microbiome in Patients with Fibromyalgia: A Pilot Study. 2024 PMID 39456224 PMC11506677 own_data
Archive description
Abstract: Fibromyalgia (FM) is a chronic disorder characterized by widespread musculoskeletal pain often accompanied by fatigue, sleep disturbances, memory issues, and mood disorders. The exact cause of FM remains unknown, and diagnosis is typically based on a history of persistent widespread pain, as there are no objective biomarkers usable in diagnosis of this disorder available. The aim of this study was to identify measurable indicators specific to FM with potential as biomarkers. Methods: This study included 17 individuals diagnosed with FM and 24 apparently healthy persons. Using real-time polymerase chain reaction (qPCR), we detected the presence of human herpesvirus (HHV)-6A and B genomic sequences in DNA isolated from peripheral blood mononuclear cells (PBMC) and buccal swabs. HHV-6-specific IgG and IgM class antibodies, along with proinflammatory cytokine levels, were measured using enzyme-linked immunosorbent assay (ELISA) and bead-based multiplex assays. Additionally, the gut microbiome was analyzed through next-generation sequencing. Results: HHV-6B was more frequently detected in the PBMCs of FM patients. FM patients with a Body Mass Index (BMI) of 30 or higher exhibited elevated cytokine levels compared to the control group with the same BMI range. Gut microbiome analysis revealed significant differences in both α-diversity and β-diversity between the FM and control groups, indicating a shift in species abundance in the FM group.
How this project entered the registry
Classified as human gut / stool / oral cavity / blood / tissue / internal fluids shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Fibromyalgia and gut microbiome”
- body site study.description · “assays. Additionally, the gut microbiome was analyzed”
- body site study.description · “cells (PBMC) and buccal swabs. HHV-6-specific IgG”
- body site study.description · “isolated from peripheral blood mononuclear cells (PBMC)”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA116093487 | antibiotic_exposure | no | R2 | 0.75 | external.gmrepo source | Recent_Antibiotics_Use: No |
| SAMEA116093473 | collection_date | 2023-01 | R1 | 0.90 | biosample.attribute:collection_date | 2023-01 |
| SAMEA116093473 | country | LV | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Latvia |
| SAMEA116093473 | health_condition | other_disease | R1 | 0.85 | biosample.attribute:DESCRIPTION | Female, Fibromyalgia group |
| SAMEA116093473 | health_condition_detail | Female, Fibromyalgia group | R1 | 0.85 | biosample.attribute:DESCRIPTION | Female, Fibromyalgia group |
| SAMEA116093473 | sex | female | R1 | 0.85 | biosample.attribute:DESCRIPTION | Female, Fibromyalgia group |
| SAMEA116093473 | subject_id | SAMEA116093473 | R4 | 0.60 | design.one_sample_per_subject:abstract | This study included 17 individuals diagnosed with FM and 24 apparently healthy persons. |
Sequencing
41 runs over 41 samples · mean 5.65 Gbp per run (median 4.88 Gbp, total 231.55 Gbp; 41 runs with a base count) · layout PAIRED 41 · platform DNBSEQ 41 · models DNBSEQ-G400 (41) · 0% of runs with a Sandpiper profile · first public 2024-09-26
Authors (10)
Ievina L first · Fomins N · Gudra D · Kenina V · Vilmane A · Gravelsina S · Rasa-Dzelzkaleja S · Murovska M · Fridmanis D · Nora-Krukle Z last
Downloads
PRJEB80379.csv.gz (1.9 KB) · parquet (71.4 KB) — all 41 samples, every field with route and confidence · PRJEB80379_determinations.csv.gz (2.7 KB) — 264 evidence rows