Fiber mixture crossover trial in Dutch adults with metabolic risk
238 faecal samples from healthy Dutch adults (45–70 years) with increased metabolic risk, in a randomized, double-blind, placebo-controlled cross-over study of a daily acacia gum and carrot powder fibre mixture versus placebo. machine-written summary
238 samples · 238 runs · NL · Adult · 2025 · 0.42 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 2,618 sample values (age, collection date, country, intervention, intervention detail, latitude, location region, longitude) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 476 sample values (health condition, health condition detail) · PMC12756460 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
Prebiotic / dietary fibre supplement design crossover · population cardiometabolic_other · Acacia gum and carrot powder fibre mixture vs placebo, 12 weeks crossover
“Subjects took daily a fibre mixture of Acacia gum and carrot powder” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal
61 of 62 people sampled more than once (237 samples; median 4, up to 4 per person) · follow-up median 3 mo (max 3 mo) · median interval between samples 3 mo (time from timepoint numbers, unit from the stated sampling schedule). Schedule stated by the source: “Stool collected at baseline and every 4 weeks (0,4,8,12wk) in each of two 12-week crossover periods, separated by an 8-week washout” (time origin: intervention_start); stated follow-up 7 mo.
Each row is one person (36 shown of 61); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 208 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 238 | 100% | ||||
| country | 238 | 100% | 238 | |||
| health_condition | 238 | 100% | 238 | |||
| subject_id | 238 | 100% | 238 | |||
| age_at_collection_days | 238 | 100% | 238 | |||
| collection_date | 238 | 100% | 238 | |||
| detailed_location | 238 | 100% | ||||
| health_condition_detail | 238 | 100% | 238 | |||
| intervention | 238 | 100% | 238 | |||
| intervention_detail | 238 | 100% | 238 | |||
| latitude | 238 | 100% | 238 | |||
| location_region | 238 | 100% | 238 | |||
| longitude | 238 | 100% | 238 | |||
| sex | 238 | 100% | 238 | |||
| timepoint_label | 238 | 100% | 238 |
- Translating <i>in vitro</i> gut microbiota models to human context: compositional correlations under dietary fiber intervention. 2025 PMID 41488312 PMC12756460 own_data
Archive description
This randomized, double-blind, cross-over study included 65 healthy subjects, aged 45–70 years, with increased metabolic risk (i.e., body mass index [BMI] 25–30 kg/m2, low to moderate daily dietary fibre intake, <30g/day). Subjects took daily a fibre mixture of Acacia gum and carrot powder or placebo for 12 weeks, with an 8-week wash-out period. Faecal samples for measurement of SCFAs and microbiome analysis were collected every 4 weeks. The fibre mixture exerted microbiome modulation, with an increase in β-diversity (p < 0.001). α-diversity was lower during fibre mixture intake compared to placebo after 4, 8 and 12 weeks (p = 0.002 p = 0.012 p = 0.031). Faecal samples taken at baseline of the intervention period were used for culturing experiments in which the microbiome modulating effects of fiber exposure in vitro were determined.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult and elderly by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Fiber Mixture on Gut Microbiome Diversity and”
- body site study.description · “8-week wash-out period. Faecal samples for measurement”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- life stage study.title · “Fatty Acids in Adults with Increased Metabolic”
- life stage sample.attr.age · “68 (n=53/238)”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMEA117395231 | age_at_collection_days | 23010.8 | R1 | 0.70 | sample.attr.age | 63 |
| SAMEA117395231 | collection_date | 2020-08-01 | R1 | 0.90 | biosample.attribute:collection_date | 2020-08-01 |
| SAMEA117395231 | country | NL | R1 | 0.90 | sample.attr.geographic_location_country_and_or_sea | Netherlands |
| SAMEA117395231 | health_condition | healthy_control | R3 | 0.70 | paper.fulltext.abstract | healthy volunteers ( N = 54, 45–70 years |
| SAMEA117395231 | health_condition_detail | healthy volunteers with increased metabolic risk (BMI 25-30) | R3 | 0.70 | paper.fulltext.abstract | healthy volunteers ( N = 54, 45–70 years |
| SAMEA117395231 | intervention | no_intervention | R1 | 0.80 | biosample.attribute:collection timepoint weeks | collection timepoint weeks: 0 |
| SAMEA117395233 | intervention | prebiotic_or_fibre | R1 | 0.90 | biosample.attribute:treatment | treatment: Fiber mix |
| SAMEA117395231 | intervention_detail | collection timepoint weeks = 0 (before intervention start); assigned arm: placebo control period | R1 | 0.80 | biosample.attribute:collection timepoint weeks | collection timepoint weeks: 0 |
| SAMEA117395233 | intervention_detail | acacia gum and carrot powder fibre mixture | R1 | 0.90 | biosample.attribute:treatment | treatment: Fiber mix |
| SAMEA117395231 | latitude | 52.1667 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 52.166661 4.469690 |
| SAMEA117395231 | location_region | South Holland | R1 | 0.60 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 52.166661 4.469690 |
| SAMEA117395231 | longitude | 4.4697 | R1 | 0.90 | biosample.attribute:geographic_location_latitude+geographic_location_longitude | 52.166661 4.469690 |
| SAMEA117395231 | sex | male | R1 | 0.90 | sample.attr.sex | Male |
| SAMEA117395231 | subject_id | 22 | R1 | 0.85 | sample.attr.subject | 22 |
| SAMEA117395231 | timepoint_label | 0 | R1 | 0.85 | sample.attr.collection_timepoint_weeks | 0 |
Sequencing
238 runs over 238 samples · mean 0.44 Gbp per run (median 0.42 Gbp, total 105.02 Gbp; 238 runs with a base count) · layout PAIRED 238 · platform ILLUMINA 238 · models NextSeq 500 (238) · 87% of runs with a Sandpiper profile · first public 2025-03-20
Authors (9)
Hoevenaars FPM first · Scheithauer TPM · Eveleens Maarse BC · de Oliveira IM · Warnke I · Sybesma W · Moerland M · van den Broek TJ · Schuren FHJ last
Downloads
PRJEB81519.csv.gz (7.4 KB) · parquet (80.4 KB) — all 238 samples, every field with route and confidence · PRJEB81519_determinations.csv.gz (18.1 KB) — 3,094 evidence rows