Gut microbiota in HLA-B27 ankylosing spondylitis, Chengdu
88 fecal samples from adults in Chengdu, China, comparing HLA-B27-positive AS patients, HLA-B27-positive healthy controls, and HLA-B27-negative healthy controls in a case-control study of gut microbiome and metabolome changes. machine-written summary
88 samples · 88 runs · CN · Adult · 2026 · 6.49 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 648 sample values (collection date, country, health condition, health condition detail, latitude, location locality, location region, longitude) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 264 sample values (antibiotic exposure, location site, subject id) · PMC12915779 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Antibiotic given as the study intervention (incl. eradication therapy design observational_with_procedure · population autoimmune_inflammatory · Human fecal metagenomics cross-sectional; mouse FMT from clinical donors into antibiotic-treated mice
“FMT) from clinical donors into antibiotic-treated mice” — abstract:41698880 (study level; per-sample arms are in the intervention column below)
Genera, mean relative abundance over 87 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 88 | 100% | ||||
| country | 88 | 100% | 88 | |||
| health_condition | 60 | 68% | 60 | |||
| subject_id | 88 | 100% | 88 | |||
| antibiotic_exposure | 88 | 100% | 88 | |||
| collection_date | 88 | 100% | 88 | |||
| detailed_location | 88 | 100% | ||||
| latitude | 88 | 100% | 88 | |||
| location_locality | 88 | 100% | 88 | |||
| location_region | 88 | 100% | 88 | |||
| location_site | 88 | 100% | 88 | |||
| longitude | 88 | 100% | 88 | |||
| health_condition_detail | 60 | 68% | 60 |
- HLA-B27-associated gut microbiota and amino acid perturbations promote ankylosing spondylitis through M1 macrophage activation. 2026 PMID 41698880 PMC12915779 own_data
Archive description
Utilizing multi-omics data, our study has provided evidence that HLA-B27 may influence the gut ecosystem by altering the composition of the microbiome and its metabolic functions. We identified disturbances in three amino acid metabolism pathways, including tryptophan metabolism, cysteine metabolism, and biosynthesis of branched-chain amino acid, ornithine, and lysine, as hallmark features in the gut ecosystem of AS. Moreover, our correlation analysis led to the discovery of several novel AS-related species and metabolites. Collectively, these findings highlight the potential role of HLA-B27 in the pathogenesis of AS, as seen through the lens of gut microbiome changes. The further disruption of microbiota-derived metabolism in the context of HLA-B27 may be partially responsible for the eventual development of AS among HLA-B27-positive individuals. Our study also opens up new directions to develop novel therapeutic strategies for AS however, these findings will require further investigation and verification.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_adult_only”
- body site study.title · “Metagenome AS and Gut microbiota”
- body site study.description · “may influence the gut ecosystem by altering”
- life stage external_curation.infant_catalog · “infant triage reason_code age_adult_only”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN36958563 | antibiotic_exposure | no | R3 | 0.70 | paper.fulltext.methods | taken antibiotics or probiotics within three months |
| SAMN36958563 | collection_date | 2022-08-25 | R1 | 0.90 | biosample.attribute:collection_date | 2022-08-25 |
| SAMN36958563 | country | CN | R1 | 0.90 | sample.attr.geo_loc_name | China:Chengdu |
| SAMN36958563 | health_condition | healthy_control | R1 | 0.70 | sample.name_rule:alias | B27N_control_1 |
| SAMN36958563 | health_condition_detail | B27N_control | R1 | 0.70 | sample.name_rule:alias | B27N_control_1 |
| SAMN36958563 | latitude | 30.6100 | R1 | 0.90 | biosample.attribute:lat_lon | 30.61 N 104.03 E |
| SAMN36958563 | location_locality | Chengdu | R1 | 0.85 | biosample.attribute:geo_loc_name | China:Chengdu |
| SAMN36958563 | location_region | Sichuan | R1 | 0.60 | biosample.attribute:lat_lon | 30.61 N 104.03 E |
| SAMN36958563 | location_site | West China Hospital of Sichuan University | R3 | 0.70 | paper.fulltext.methods | Biomedical Research Ethics Committee of West China Hospital of Sichuan University |
| SAMN36958563 | longitude | 104.0300 | R1 | 0.90 | biosample.attribute:lat_lon | 30.61 N 104.03 E |
| SAMN36958563 | subject_id | SAMN36958563 | R3 | 0.70 | design.one_sample_per_subject:fulltext | A cohort of 28 AS patients and 60 HCs, ranging in age from 18 to 65 y, was recruited for the study. |
Sequencing
88 runs over 88 samples · mean 6.47 Gbp per run (median 6.49 Gbp, total 569.65 Gbp; 88 runs with a base count) · layout PAIRED 88 · platform ILLUMINA 88 · models Illumina NovaSeq 6000 (88) · 99% of runs with a Sandpiper profile · first public 2026-01-01
Authors (10)
Huang T first · Yang H · Zhang L · Wang X · Chen Y · Dai H · Hashimoto K · Luo Y · Pu Y · Liu Y last
Downloads
PRJNA1004858.csv.gz (3.2 KB) · parquet (75.0 KB) — all 88 samples, every field with route and confidence · PRJNA1004858_determinations.csv.gz (6.6 KB) — 912 evidence rows