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PRJNA1008138 SRP474381

Human and mouse lemur fecal metagenomes, Madagascar

Fecal metagenomes from humans and mouse lemurs

Cross-sectional study of 57 fecal metagenome samples from sympatric humans and wild gray mouse lemurs near Ranomafana National Park, Madagascar, comparing gut microbiome bacterial species and antibiotic resistance genes. machine-written summary

57 samples · 68 runs · MG · Age unknown · 2023 · 2.20 Gbp / sample

Sources
✓Sequence archive 171✓Abstract / description 114✓Full text 57✓Supplementary tables 57–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive171 sample values (collection date, country, location site)
✓ Abstract / description114 sample values (health condition detail, lifestyle detail)
✓ Full text57 sample values (location region) · PMC11296303
✓ Supplementary tables57 sample values (subject id)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet · share metadata
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
unknown57
Country
MG57
Body site
primary57
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)all projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 54 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 3 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 2.06 Gbp per sample0130Gbp
Sampling design and relatedness

households — not resolved

Humans sampled by household in 8 communities near Ranomafana NP (plus lemurs); household ids not published.

How longitudinal and relatedness information is derived

Community composition
Prevotella 31.9%Faecalibacterium 5.5%Staphylococcus 3.3%Roseburia 3.2%Alloprevotella 2.7%Bifidobacterium 2.0%Succinivibrio 2.0%Blautia_A 1.7%Cryptobacteroides 1.4%Dialister 1.1%Streptococcus 1.0%Acetatifactor 1.0%not assigned to a genus 10.5%other genera 32.7%

Genera, mean relative abundance over 64 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country57 100%57
health_condition0 0%
subject_id57 100%57
collection_date57 100%57
detailed_location57 100%
health_condition_detail57 100%57
lifestyle_detail57 100%57
location_region57 100%57
location_site57 100%57
Papers (1)
  • Metagenome-wide characterization of shared antimicrobial resistance genes in sympatric people and lemurs in rural Madagascar. 2024 PMID 39099658 PMC11296303 own_data
Details
Archive description

Collection of human and wild gray mouse lemur fecal metagenomes collected in 2017

How this project entered the registry

Classified as human and non-human (mixed) gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.68. Assay shotgun_dna.

  • host study.title · “Fecal metagenomes from humans and mouse lemurs”
  • host study.description · “Collection of human and wild gray mouse lemur fecal metagenomes”
  • body site run.scientific_name · “gut metagenome”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN37113226collection_date2017-06-09R10.90biosample.attribute:collection_date2017-06-09
SAMN37113226countryMGR10.90sample.attr.geo_loc_nameMadagascar: Ranomafana National Park
SAMN37113226health_condition_detailmixed_case_control: Study compares two distinct host species (humans and lemurs); no health status specified for either R40.50paper.abstracthumans and wild mouse lemurs
SAMN37113226lifestyle_detailrural Madagascar, forest-dominated roadless region near Ranomafana National ParkR40.50paper.abstractrural Madagascar
SAMN37113226location_regionIfanadiana DistrictR30.70paper.fulltext.methodsOne Health research platform in Ifanadiana District, Madagascar
SAMN37113226location_siteRanomafana National ParkR10.85biosample.attribute:geo_loc_nameMadagascar: Ranomafana National Park
SAMN37113226subject_idHUM0038R20.85paper.supp.peerj-12-17805-s001.csv[!Study ID]HUM0038
Sequencing

68 runs over 57 samples · mean 2.26 Gbp per run (median 2.20 Gbp, total 153.49 Gbp; 68 runs with a base count) · layout PAIRED 68 · platform ILLUMINA 68 · models NextSeq 2000 (68) · 94% of runs with a Sandpiper profile · first public 2023-11-28 – 2024-02-25

Authors (9)

Talbot BM first · Clennon JA · Rakotoarison MFN · Rautman L · Durry S · Ragazzo LJ · Wright PC · Gillespie TR · Read TD last

Help complete this project

2 of the 4 core fields are below 50% coverage: age_category, health_condition. Share metadata

Downloads

PRJNA1008138.csv.gz (2.7 KB) · parquet (73.0 KB) — all 57 samples, every field with route and confidence · PRJNA1008138_determinations.csv.gz (3.6 KB) — 399 evidence rows

Samples (57 · every field this project has values for · hover a cell for its source)
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