Gut metagenome of healthy adults, whey protein trial, India
30 fecal samples from healthy adult males in India in a double-blind, randomized, crossover trial testing whey protein with an enzyme-probiotic blend versus placebo, with fecal microbiota assessed on day 1 and day 15. machine-written summary
30 samples · 30 runs · IN · Adult · 2024 · 0.32 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 180 sample values (collection date, country, latitude, longitude, subject id, timepoint) |
| ✓ Abstract / description | 60 sample values (health condition, health condition detail) |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention — arms not resolved
Non-microbial dietary supplement (vitamins Synbiotic (probiotic + prebiotic) design crossover · population healthy_control · Whey protein + Pepzyme Pro (enzyme-probiotic blend) vs whey protein + maltodextrin placebo, 15 days
“whey protein and Pepzyme Pro (enzyme and probiotics supplement) or placebo supplement” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal — not resolved
15 of 15 people sampled more than once (30 samples; median 2, up to 2 per person). Schedule stated by the source: “Fecal samples on day 1 and day 15 of each arm; 15-day supplement period with 30-day washout between crossover arms” (time origin: intervention_start).
No Sandpiper profile for this project's samples yet.
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 30 | 100% | ||||
| country | 30 | 100% | 30 | |||
| health_condition | 30 | 100% | 30 | |||
| subject_id | 30 | 100% | 30 | |||
| collection_date | 30 | 100% | 30 | |||
| health_condition_detail | 30 | 100% | 30 | |||
| latitude | 30 | 100% | 30 | |||
| longitude | 30 | 100% | 30 | |||
| timepoint_label | 30 | 100% | 30 |
Archive description
The double blind, cross over and randomized clinical study was designed on 15 healthy male subjects. The subjects were supplement with test supplement i.e., whey protein and Pepzyme Pro (enzyme and probiotics supplement) or placebo supplement i.e., whey protein and maltodextrin for 15 days with the washout period of 30 days. On day 1 and day 15, blood samples were drawn at 0, 30, 60, 120, 180, 240 min of post ingestion of the supplement for the analysis of plasma free amino acids. On day 15, blood samples at 0h and 4h of post ingestion of protein were tested for insulin and CRP level. Additionally, on day 1 and day 15 urine and fecal samples were evaluated for nitrogen level and the fecal samples were tested for gut microbiota.
How this project entered the registry
Classified as human gut / stool / vaginal / urogenital / blood / tissue / internal fluids shotgun DNA metagenome of adult by deterministic_prior with confidence 0.90. Assay shotgun_dna.
- host external_curation.infant_catalog · “infant triage reason_code age_unknown_no_evidence”
- body site study.title · “Metabolomics and gut metagenomics profile of”
- body site study.description · “15 urine and fecal samples were evaluated”
- body site study.description · “and day 15 urine and fecal samples”
- body site study.description · “and day 15, blood samples were drawn”
- life stage study.title · “of the healthy adults on consumption of”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN37663762 | collection_date | 2021-12-02/2022-01-10 | R1 | 0.90 | biosample.attribute:collection_date | 2021-12-02/2022-01-10 |
| SAMN37663762 | country | IN | R1 | 0.90 | sample.attr.geo_loc_name | India |
| SAMN37663762 | health_condition | healthy_control | R4 | 0.50 | study.description | 15 healthy male subjects |
| SAMN37663762 | health_condition_detail | healthy | R4 | 0.50 | study.description | healthy male subjects |
| SAMN37663762 | latitude | 20.5937 | R1 | 0.90 | biosample.attribute:lat_lon | 20.5937 N 78.9629 E |
| SAMN37663762 | longitude | 78.9629 | R1 | 0.90 | biosample.attribute:lat_lon | 20.5937 N 78.9629 E |
| SAMN37663762 | subject_id | 10 | R1 | 0.70 | sample.name_rule:title | WP_Post_10 |
| SAMN37663762 | timepoint_label | Post | R1 | 0.70 | sample.name_rule:title | WP_Post_10 |
Sequencing
30 runs over 30 samples · mean 0.37 Gbp per run (median 0.32 Gbp, total 11.14 Gbp; 30 runs with a base count) · layout SINGLE 30 · platform OXFORD_NANOPORE 30 · models MinION (30) · 0% of runs with a Sandpiper profile · first public 2024-02-07 – 2024-02-25
Authors (0)
No author record.
Downloads
PRJNA1023559.csv.gz (1.6 KB) · parquet (70.7 KB) — all 30 samples, every field with route and confidence · PRJNA1023559_determinations.csv.gz (2.2 KB) — 240 evidence rows