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PRJNA1026631 SRP467044

Female urogenital and rectal microbiomes in UTI symptoms, USA

Female Urogenital & Rectal Microbiomes of Individuals with UTI Symptoms Metagenome

Cross-sectional study of 83 samples from urogenital and rectal swab sites in females with UTI symptoms, collected in Maywood, IL, USA, including elderly and adult age groups. machine-written summary

83 samples · 83 runs · US · Elderly · 2023 · 1.67 Gbp / sample

Sources
✓Sequence archive 910–Abstract / description–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive910 sample values (age, collection date, country, health condition, health condition detail, latitude, location locality, location region)
– Abstract / descriptionno abstract or project description
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
elderly49
adult33
unknown1
Condition
other_infection83
Sex
female82
Country
US83
Body site
primary44
unknown39
Collected 2021–2022
2021: 61 samples2022: 22 samples20212022
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)all projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 10 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 70 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 3 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 1.67 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal

20 of 20 people sampled more than once (82 samples; median 4, up to 6 per person) · follow-up median 1 d (max 1 d) · median interval between samples 1 d (time from collection dates).

Participant1Participant1: day 0Participant1: day 0Participant1: day 1Participant1: day 1Participant1: day 1Participant1: day 10 d0 d0 d1 d1 d

Each row is one person (1 shown of 20); dots are samples at days since that person's first sample.

How longitudinal and relatedness information is derived

Community composition
Escherichia 46.3%Streptococcus 23.6%Enterococcus 9.2%Lactobacillus 3.3%Enterococcus_B 2.9%Staphylococcus 2.9%Bacteroides 1.8%Ligilactobacillus 0.9%ECMA0423 0.8%Bifidobacterium 0.4%Phocaeicola 0.3%Peptoniphilus_A 0.3%not assigned to a genus 3.8%other genera 3.5%

Genera, mean relative abundance over 76 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category82 99%
country83 100%83
health_condition83 100%83
subject_id82 99%82
collection_date83 100%83
detailed_location83 100%
health_condition_detail83 100%83
latitude83 100%83
location_locality83 100%83
location_region83 100%83
longitude83 100%83
age_at_collection_days82 99%82
sex82 99%82
Details
Archive description

This study is aimed at identifying the microbes in urogenital samples as well as a rectal swab of females with UTI symptoms.

How this project entered the registry

Classified as human vaginal / urogenital / gut / stool shotgun DNA metagenome of elderly and adult by opus_adjudicated with confidence 0.75. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • body site study.description · “urogenital samples as well as a rectal swab”
  • body site run.scientific_name · “urine metagenome”
  • body site run.scientific_name · “feces metagenome”
  • life stage sample.attr.host_age · “68 (n=51/83)”
  • life stage sample.attr.host_age · “51 (n=31/83)”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN37835371age_at_collection_days28854.8R10.70sample.attr.host_age79
SAMN37753975collection_date2021-05-19R10.90biosample.attribute:collection_date2021-05-19
SAMN37753975countryUSR10.90sample.attr.geo_loc_nameUSA: Maywood, IL
SAMN37753975health_conditionother_infectionR10.85biosample.attribute:urogenit_tract_disorUTI
SAMN37753975health_condition_detailUTIR10.90sample.attr.urogenit_tract_disorUTI
SAMN37753975latitude41.8600R10.90biosample.attribute:lat_lon41.86 N 87.84 W
SAMN37753975location_localityMaywoodR10.85biosample.attribute:geo_loc_nameUSA: Maywood, IL
SAMN37753975location_regionIllinoisR10.75biosample.attribute:geo_loc_nameUSA: Maywood, IL
SAMN37753975longitude-87.8400R10.90biosample.attribute:lat_lon41.86 N 87.84 W
SAMN37835371sexfemaleR10.90sample.attr.host_sexfemale
SAMN37753975subject_idParticipant1R10.70sample.name_rule:aliasParticipant1_Bladder_LB
Sequencing

83 runs over 83 samples · mean 1.73 Gbp per run (median 1.67 Gbp, total 143.25 Gbp; 83 runs with a base count) · layout PAIRED 83 · platform ILLUMINA 83 · models NextSeq 2000 (83) · 94% of runs with a Sandpiper profile · first public 2023-10-22

Authors (0)

No author record.

Downloads

PRJNA1026631.csv.gz (3.3 KB) · parquet (74.6 KB) — all 83 samples, every field with route and confidence · PRJNA1026631_determinations.csv.gz (6.8 KB) — 910 evidence rows

Samples (83 · every field this project has values for · hover a cell for its source)
Loading the sample table…