IgA coating of gut microbiota in healthy California adults
Cross-sectional study using metagenomic immunoglobulin sequencing (MIg-seq) on 76 stool samples from healthy adults in California, USA, to determine strain-level IgA coating levels across gut microbiome strains. machine-written summary
76 samples · 76 runs · US · Adult · 2023 · 16.25 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 532 sample values (collection date, country, latitude, location region, longitude, subject id, timepoint) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 76 sample values (health condition) · PMC11849979 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
Genera, mean relative abundance over 69 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 76 | 100% | ||||
| country | 76 | 100% | 76 | |||
| health_condition | 76 | 100% | 76 | |||
| subject_id | 76 | 100% | 76 | |||
| collection_date | 76 | 100% | 76 | |||
| detailed_location | 76 | 100% | ||||
| latitude | 76 | 100% | 76 | |||
| location_region | 76 | 100% | 76 | |||
| longitude | 76 | 100% | 76 | |||
| timepoint_label | 76 | 100% | 76 |
- Metagenomic immunoglobulin sequencing reveals IgA coating of microbial strains in the healthy human gut. 2025 PMID 39747692 PMC11849979 own_data
Archive description
Data supporting the publication "Metagenomic Immunoglobulin Sequencing (MIG-Seq) Exposes Patterns of IgA Antibody Binding in the Healthy Human Gut Microbiome". Abstract: IgA, the most highly produced human antibody, is continually secreted into the gut to shape the intestinal microbiota. Methodological limitations have critically hindered defining which microbial strains are targeted by IgA and why. Here, we develop a new technique, Metagenomic Immunoglobulin Sequencing (MIG-Seq), and use it to determine IgA coating levels for thousands of gut microbiome strains in healthy humans. We find that microbes associated with both health and disease have higher levels of coating, and that microbial genes are highly predictive of IgA binding levels, with mucus degradation genes especially correlated with high binding. We find a significant reduction in replication rates among microbes bound by IgA, and demonstrate that IgA binding is more correlated with host immune status than traditional microbial abundance measures. This study introduces a powerful technique for assessing strain-level IgA binding in human stool, paving the way for deeper understanding of IgA-based host microbe interactions.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.68. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site run.scientific_name · “human gut metagenome”
- life stage study.description · “gut microbiome strains in healthy humans”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN38698739 | collection_date | 2017 | R1 | 0.90 | biosample.attribute:collection_date | 2017 |
| SAMN38698739 | country | US | R1 | 0.90 | sample.attr.geo_loc_name | USA: California |
| SAMN38698739 | health_condition | healthy_control | R3 | 0.70 | paper.fulltext.abstract | IgA coating levels for 3,520 gut microbiome strains in healthy human feces |
| SAMN38698739 | latitude | 37.4275 | R1 | 0.90 | biosample.attribute:lat_lon | 37.4275 N 122.1697 W |
| SAMN38698739 | location_region | California | R1 | 0.85 | biosample.attribute:geo_loc_name | USA: California |
| SAMN38698739 | longitude | -122.1697 | R1 | 0.90 | biosample.attribute:lat_lon | 37.4275 N 122.1697 W |
| SAMN38698739 | subject_id | 1018_p | R1 | 0.85 | sample.attr.host_subject_id | 1018_p |
| SAMN38698739 | timepoint_label | 1 | R1 | 0.70 | sample.name_rule:title | Subject 8017; timepoint 1; positive IgA |
Sequencing
76 runs over 76 samples · mean 16.23 Gbp per run (median 16.25 Gbp, total 1,233.76 Gbp; 76 runs with a base count) · layout PAIRED 76 · platform ILLUMINA 76 · models Illumina NovaSeq 6000 (76) · 91% of runs with a Sandpiper profile · first public 2023-12-07 – 2023-12-10
Authors (5)
Olm MR first · Spencer SP · Takeuchi T · Silva EL · Sonnenburg JL last
Downloads
PRJNA1049470.csv.gz (2.9 KB) · parquet (73.4 KB) — all 76 samples, every field with route and confidence · PRJNA1049470_determinations.csv.gz (5.2 KB) — 608 evidence rows