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PRJNA1064103 SRP483368

Gut microbiome in colonoscopy rinsates from IBD patients, USA

Metagenomics of Colonoscopic Rinsates: Investigating Microbial Patterns in Inflammatory Bowel Disease

Shotgun metagenomic sequencing of 80 colonoscopic rinsate samples from US patients with Crohn's disease (n=50) or ulcerative colitis (n=15), examining microbiome patterns in inflammatory bowel disease. machine-written summary

80 samples · 80 runs · US · Age unknown · 2024 · 0.55 Gbp / sample

Sources
✓Sequence archive 290–Abstract / description–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive290 sample values (collection date, country, health condition, health condition detail)
– Abstract / descriptionno abstract or project description
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet · share metadata
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
unknown80
Condition
crohns_disease50
ulcerative_colitis15
Country
US80
Body site
primary80
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)this project, 0–0.03 Gbp: 3 samplesall projects, 0.03–0.1 Gbp: 17,066 samples (3 %)this project, 0.03–0.1 Gbp: 2 samplesall projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 4 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 62 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 8 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 1 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 0.55 Gbp per sample0130Gbp
Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category0 0%
country80 100%80
health_condition65 81%65
subject_id0 0%
collection_date80 100%80
health_condition_detail65 81%65
Details
Archive description

The association between the microbiome and Inflammatory Bowel Disease by analyzing 80 colonscopy rinsate samples from intestinal microfistulous tracts, suggesting the potential influence of microbiome commensals on the inflammatory processes in Crohn's disease and Ulcerative colitis patients.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by curator_audit with confidence 0.60. Assay shotgun_dna.

  • host runs.isolation_source · “Colonoscopic rinsates”
  • host runs.scientific_name · “human gut metagenome”
  • body site runs.isolation_source · “Colonoscopic rinsates”
  • body site runs.scientific_name · “human gut metagenome”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN39418236collection_date2021-06-07R10.85biosample.attribute:collection_datecollection_date: 2021-06-07
SAMN39418236countryUSR10.90sample.attr.geo_loc_nameUSA: Ohio, Cleveland
SAMN39418236health_conditioncrohns_diseaseR10.70sample.name_rule:library_namesCD_1
SAMN39418236health_condition_detailCrohn's diseaseR10.70sample.name_rule:library_namesCD_1
Sequencing

80 runs over 80 samples · mean 0.65 Gbp per run (median 0.55 Gbp, total 51.83 Gbp; 80 runs with a base count) · layout PAIRED 80 · platform ILLUMINA 80 · models Illumina HiSeq X (80) · 10% of runs with a Sandpiper profile · first public 2024-04-28

Authors (0)

No author record.

Help complete this project

2 of the 4 core fields are below 50% coverage: age_category, subject_id. Share metadata

Downloads

PRJNA1064103.csv.gz (2.7 KB) · parquet (72.4 KB) — all 80 samples, every field with route and confidence · PRJNA1064103_determinations.csv.gz (2.9 KB) — 290 evidence rows

Samples (80 · every field this project has values for · hover a cell for its source)
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