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PRJNA1077691 SRP527464

Metagenome-guided culturomics of healthy gut microbes, Denmark

unidentified Raw sequence reads

498 samples from healthy adult controls in Copenhagen, Denmark, used to combine whole-metagenome sequencing with culturomics to selectively enrich targeted gut microbial taxa and functions via medium modifications. machine-written summary

498 samples · 498 runs · DK · Age unknown · 2024 · 2.01 Gbp / sample

Sources
✓Sequence archive 996○Abstract / description✓Full text 1,494○Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive996 sample values (country, location locality)
○ Abstract / descriptionread; no usable statement
✓ Full text1,494 sample values (antibiotic exposure, health condition, health condition detail) · PMC11733127
○ Supplementary tablesfiles read; no table could be matched to the samples
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 country
Age
adult498
Condition
healthy_control498
Country
DK498
Body site
primary498
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)this project, 0–0.03 Gbp: 8 samplesall projects, 0.03–0.1 Gbp: 17,066 samples (3 %)this project, 0.03–0.1 Gbp: 8 samplesall projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 15 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 59 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 266 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 140 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)this project, 10–30 Gbp: 2 samplesall projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 2.01 Gbp per sample0130Gbp
Community composition
Bacteroides 30.2%Escherichia 20.3%Phocaeicola 12.3%Parabacteroides 4.4%Acidaminococcus 2.3%Bifidobacterium 2.3%Citrobacter 2.0%Bilophila 1.9%Lawsonibacter 1.7%Enterocloster 1.6%Phascolarctobacterium 1.4%Collinsella 0.8%not assigned to a genus 7.8%other genera 11.1%

Genera, mean relative abundance over 436 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).

Field coverage
fieldsamplescoverageR1R2R3R4
age_category498 100%
country498 100%498
health_condition498 100%498
subject_id0 0%
antibiotic_exposure498 100%498
detailed_location498 100%
health_condition_detail498 100%498
location_locality498 100%498
Papers (1)
Details
Archive description

Metagenome-guided culturomics for the targeted enrichment of gut microbes.

How this project entered the registry

Classified as human gut / stool shotgun DNA metagenome of age unknown by sonnet_x2 with confidence 0.53. Assay shotgun_dna.

  • host run.host_tax_id · “9606”
  • body site run.scientific_name · “gut metagenome”
  • body site study.description · “enrichment of gut microbes”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN43164958antibiotic_exposurenoR30.70paper.fulltext.methodsnot having undergone antibiotic, antiviral, antifungal
SAMN43164958countryDKR10.90sample.attr.geo_loc_nameDenmark: Copenhagen
SAMN43164958health_conditionhealthy_controlR30.70paper.fulltext.methodsselected to be considered healthy individual
SAMN43164958health_condition_detailhealthy individual, self-declared, no chronic conditionR30.70paper.fulltext.methodsselected to be considered healthy individual
SAMN43164958location_localityCopenhagenR10.85biosample.attribute:geo_loc_nameDenmark: Copenhagen
Sequencing

498 runs over 498 samples · mean 2.38 Gbp per run (median 2.01 Gbp, total 1,185.78 Gbp; 498 runs with a base count) · layout PAIRED 498 · platform ILLUMINA 498 · models Illumina NovaSeq 6000 (498) · 88% of runs with a Sandpiper profile · first public 2024-12-05

Authors (5)

Armetta J first · Li SS · Vaaben TH · Vazquez-Uribe R · Sommer MOA last

Downloads

PRJNA1077691.csv.gz (11.4 KB) · parquet (87.3 KB) — all 498 samples, every field with route and confidence · PRJNA1077691_determinations.csv.gz (12.1 KB) — 2,490 evidence rows

Samples (498 · every field this project has values for · hover a cell for its source)
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