Gut microbiome in stressed Chinese adolescents, cross-sectional
59 fecal samples from Chinese adolescents (ages 12-16), stratified by chronic stress level (low vs. high), analyzed via metagenomic sequencing and metabolomics in a cross-sectional design. machine-written summary
59 samples · 59 runs · CN · Adolescent · 2024 · 3.14 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 177 sample values (age, collection date, country) |
| ○ Abstract / description | read; no usable statement |
| ✓ Full text | 177 sample values (antibiotic exposure, location locality, location site) · PMC12235824 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet · share metadata |
| – Human verified | not yet checked by a person · I checked this project — verify it |
Genera, mean relative abundance over 57 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 59 | 100% | ||||
| country | 59 | 100% | 59 | |||
| health_condition | 0 | 0% | ||||
| subject_id | 0 | 0% | ||||
| age_at_collection_days | 59 | 100% | 59 | |||
| antibiotic_exposure | 59 | 100% | 59 | |||
| collection_date | 59 | 100% | 59 | |||
| detailed_location | 59 | 100% | ||||
| location_locality | 59 | 100% | 59 | |||
| location_site | 59 | 100% | 59 |
- Chronic stress is associated with altered gut microbiota profile and relevant metabolites in adolescents. 2025 PMID 40629292 PMC12235824 own_data
Archive description
Fecal samples from 124 adolescents aged 12-16 years in China were collected for 16S rRNA sequencing, metagenomic sequencing, and liquid chromatography-mass spectrometry. Chronic stress was measured by the Adolescent Life Events Scale and the Study Stress Scale.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adolescent by sonnet_x2 with confidence 0.75. Assay shotgun_dna.
- host study.title · “Homo sapiens Raw sequence reads”
- body site study.description · “Fecal samples from 124 adolescents”
- life stage study.description · “adolescents aged 12-16 years”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN39981088 | age_at_collection_days | 5113.0 | R1 | 0.85 | sample.attr.age | 14 |
| SAMN39981088 | antibiotic_exposure | no | R3 | 0.70 | paper.fulltext.methods | taken antibiotics, probiotics, or prebiotics within the past three months |
| SAMN39981088 | collection_date | 2022-12 | R1 | 0.90 | biosample.attribute:collection_date | 2022-12 |
| SAMN39981088 | country | CN | R1 | 0.90 | sample.attr.geo_loc_name | China |
| SAMN39981088 | location_locality | Xi'an | R3 | 0.70 | paper.fulltext.methods | a middle school in Xi’an, China |
| SAMN39981088 | location_site | middle school in Xi'an | R3 | 0.70 | paper.fulltext.methods | a middle school in Xi’an, China |
Sequencing
59 runs over 59 samples · mean 3.65 Gbp per run (median 3.14 Gbp, total 215.54 Gbp; 59 runs with a base count) · layout SINGLE 59 · platform ILLUMINA 59 · models Illumina NovaSeq 6000 (59) · 97% of runs with a Sandpiper profile · first public 2024-03-06
Authors (16)
Ying L first · Yuhao W · Yafang H · Jiao L · Lina D · Qinze S · Chenghai Y · Shaoxiong Z · Yuexi G · Mingwang S · Zelin C · Chuangchuang W · Zihan G · Xin L · Lu M · Lei Z last
Help complete this project
2 of the 4 core fields are below 50% coverage: health_condition, subject_id. Share metadata
Downloads
PRJNA1077752.csv.gz (2.3 KB) · parquet (71.6 KB) — all 59 samples, every field with route and confidence · PRJNA1077752_determinations.csv.gz (2.2 KB) — 354 evidence rows