Severe alcoholic hepatitis, Amiens, pre/post steroids
54 fecal metagenome samples from 27 severe alcoholic hepatitis patients at Amiens University Hospital, France, collected before and after a 7-day corticosteroid therapy course (longitudinal design). machine-written summary
54 samples · 54 runs · FR · Adult · 2024 · 1.85 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 518 sample values (collection date, country, health condition, intervention, intervention detail, latitude, location locality, longitude) |
| ✓ Abstract / description | 76 sample values (health condition, health condition detail) |
| ✓ Full text | 54 sample values (location site) · PMC11290774 |
| ○ Supplementary tables | files read; no table could be matched to the samples |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
intervention · arms resolved
27 people have samples in more than one arm.
Non-antibiotic pharmaceutical given as the study intervention (metformin design single_arm_before_after · population liver_disease · 7-day corticosteroid (prednisolone) therapy in severe alcoholic hepatitis
“before (D0) and after (D7) a 7-day corticotherapy course” — bioproject.description (study level; per-sample arms are in the intervention column below)
longitudinal
27 of 27 people sampled more than once (54 samples; median 2, up to 2 per person) · follow-up median 7 d (max 7 d) · median interval between samples 7 d (time from collection dates). Schedule stated by the source: “stool samples at D0 (before) and D7 (after 7-day corticotherapy course)” (time origin: treatment_start); stated follow-up 7 d.
Each row is one person (27 shown); dots are samples at days since that person's first sample.
Genera, mean relative abundance over 51 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 54 | 100% | ||||
| country | 54 | 100% | 54 | |||
| health_condition | 54 | 100% | 32 | 22 | ||
| subject_id | 54 | 100% | 54 | |||
| collection_date | 54 | 100% | 54 | |||
| detailed_location | 54 | 100% | ||||
| health_condition_detail | 54 | 100% | 54 | |||
| intervention | 54 | 100% | 54 | |||
| intervention_detail | 54 | 100% | 54 | |||
| latitude | 54 | 100% | 54 | |||
| location_locality | 54 | 100% | 54 | |||
| location_site | 54 | 100% | 54 | |||
| longitude | 54 | 100% | 54 | |||
| timepoint_label | 54 | 100% | 54 |
- Serum bile acids profiles are altered without change of the gut microbiota composition following a seven-day prednisolone therapy in severe alcoholic hepatitis. 2024 PMID 39078043 PMC11290774 own_data
Archive description
Severe Alcoholic Hepatitis (sAH) is an acute form of liver injury caused by chronic and heavy alcohol drinking. A one-month corticosteroids course is the only sAH reference treatment, and its interactions with the Gut Microbiota (GM), which is a key contributor to liver injury, remain unknown. To evaluate the evolution of the GM in sAH patients, we retrospectively investigated the composition of the GM of 27 sAH patients at the Amiens University Hospital before (D0) and after (D7) a 7-day corticotherapy course using fecal metagenomics sequencing. We also quantified fecal Short Chain Fatty Acids (SCFA) and fecal and serum Bile Acids (BA), as well as serum Lipopolysaccharide-Binding Protein (LBP). Overall, the community and taxonomical analyses did not reveal any GM evolution between D0 and D7, nor did the SCFA profiles analysis. However, in serum but not fecal samples, the ratio of glyco-conjugated to tauro-conjugated BA was significantly reduced at D7, independently of the response to treatment, while two BA were enriched in non-responder patients. LBP concentration significantly diminished between D0 and D7, which may indicate an improvement of the gut barrier. The stability of the GM of sAH is interesting in the perspective of new treatments based on GM modulation.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of adult by sonnet_x2 with confidence 0.70. Assay shotgun_dna.
- host run.scientific_name · “human gut metagenome”
- body site study.title · “change of the Gut Microbiota composition”
- body site study.description · “fecal metagenomics sequencing”
- life stage study.description · “Amiens University Hospital before”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN41077593 | collection_date | 2019-08-16 | R1 | 0.90 | biosample.attribute:collection_date | 2019-08-16 |
| SAMN41077593 | country | FR | R1 | 0.90 | sample.attr.geo_loc_name | France: Amiens |
| SAMN41077597 | health_condition | liver_disease | R1 | 0.70 | biosample_attr | PR_D0 |
| SAMN41077593 | health_condition | liver_disease | R4 | 0.50 | study.description | 27 sAH patients at the Amiens University Hospital |
| SAMN41077593 | health_condition_detail | Severe Alcoholic Hepatitis | R4 | 0.50 | study.description | 27 sAH patients at the Amiens University Hospital |
| SAMN41077593 | intervention | no_intervention | R1 | 0.80 | biosample.attribute:Group | Group: PNR_D0 |
| SAMN41077593 | intervention_detail | non-responder, baseline before 7-day prednisolone | R1 | 0.80 | biosample.attribute:Group | Group: PNR_D0 |
| SAMN41077593 | latitude | 49.5200 | R1 | 0.90 | biosample.attribute:lat_lon | 49.52 N 2.15 E |
| SAMN41077593 | location_locality | Amiens | R1 | 0.85 | biosample.attribute:geo_loc_name | France: Amiens |
| SAMN41077593 | location_site | Amiens University Hospital | R3 | 0.70 | paper.fulltext.abstract | 27 sAH patients at the Amiens University Hospital |
| SAMN41077593 | longitude | 2.1500 | R1 | 0.90 | biosample.attribute:lat_lon | 49.52 N 2.15 E |
| SAMN41077593 | subject_id | BB.013 | R1 | 0.85 | sample.attr.individual | BB.013 |
| SAMN41077593 | timepoint_label | D0 | R1 | 0.85 | sample.attr.time | D0 |
Sequencing
54 runs over 54 samples · mean 2.94 Gbp per run (median 1.85 Gbp, total 158.73 Gbp; 54 runs with a base count) · layout PAIRED 54 · platform ILLUMINA 54 · models NextSeq 550 (54) · 93% of runs with a Sandpiper profile · first public 2024-07-23
Authors (11)
Esparteiro D first · Fouquet G · Courtois A · Jedraszak G · Marticho L · Gourdel M · Billon-Crossouard S · Croyal M · Naassila M · Nguyen-Khac E · Marcq I last
Downloads
PRJNA1104421.csv.gz (2.9 KB) · parquet (73.5 KB) — all 54 samples, every field with route and confidence · PRJNA1104421_determinations.csv.gz (5.3 KB) — 648 evidence rows