Gut microbiome in B6/B12 deficient children, Balaghat, India
Case-control study of 64 gut metagenome samples from children (plus a few infants/adolescents) in Balaghat district, Madhya Pradesh, India, comparing malnutrition (B6/B12 deficiency) cases with healthy controls. machine-written summary
64 samples · 64 runs · IN · Child · 2026 · 7.62 Gbp / sample
Checked this project's papers and archive records yourself? Mark it human-verified.
what each source gave
| ✓ Sequence archive | 899 sample values (age, antibiotic exposure, bmi, collection date, country, diet, diet detail, health condition) |
| ○ Abstract / description | read; no usable statement |
| – Full text | no open-access full text linked |
| – Supplementary tables | no supplementary tables available (or none open access) |
| – External resources | not covered by the external resources we ingest |
| – User contribution | nothing uploaded yet |
| – Human verified | not yet checked by a person · I checked this project — verify it |
Genera, mean relative abundance over 62 profiled gut samples of this project (Sandpiper SingleM profiles, GTDB R232; low-depth and non-metagenome profiles left out).
| field | samples | coverage | R1 | R2 | R3 | R4 |
|---|---|---|---|---|---|---|
| age_category | 64 | 100% | ||||
| country | 64 | 100% | 64 | |||
| health_condition | 59 | 92% | 59 | |||
| subject_id | 0 | 0% | ||||
| age_at_collection_days | 64 | 100% | 64 | |||
| antibiotic_exposure | 64 | 100% | 64 | |||
| collection_date | 64 | 100% | 64 | |||
| detailed_location | 64 | 100% | ||||
| diet | 64 | 100% | 64 | |||
| diet_detail | 64 | 100% | 64 | |||
| health_condition_detail | 64 | 100% | 64 | |||
| latitude | 64 | 100% | 64 | |||
| location_locality | 64 | 100% | 64 | |||
| location_region | 64 | 100% | 64 | |||
| longitude | 64 | 100% | 64 | |||
| sex | 64 | 100% | 64 | |||
| bmi | 60 | 94% | 60 | |||
| lifestyle | 6 | 9% | 6 | |||
| lifestyle_detail | 6 | 9% | 6 |
- Inverse association between serum vitamin B12 level and abundance of potential B12-producing gut microbes in Indian children 2025 own_data
- Inverse Association between Serum Vitamin B<sub>12</sub> Level and Abundance of Potential B<sub>12</sub>-Producing Gut Microbes in Indian Children. 2025 PMID 41093108 own_data
Archive description
A comprehensive national nutrition survey in Indian population (CNNS-2016-18) showed</p><p>deficiency of multiple essential micronutrients were still prevalent not only in vulnerable</p><p>groups but also in the general population (Hidden-hunger). Despite public health strategies in</p><p>past several decades, the prevalence of Hidden-hunger requires more innovative</p><p>interventions.</p><p>One of the integral sources of micronutrients for human has been the gut microbiota, known</p><p>to produce some of those essential micronutrients. However, behaviour of native gut</p><p>microbiota in case of malnutrition has not been thoroughly investigated. So our hypothesis is</p><p>whether microbial dysbiosis (i.e, altered abundance and composition) contribute to the</p><p>prevalence of deficiency of essential micronutrients?</p><p>To address this gaps, we sequenced gut-metagenome of healthy and B6 and B12 deficient children from Balaghat district of Madhta Pradesh, India. Our analysis answerd if change in composition and dynamics of gut microbe(s) underlies micronutrient deficiency.
How this project entered the registry
Classified as human gut / stool shotgun DNA metagenome of child by sonnet_x2 with confidence 0.60. Assay shotgun_dna.
- host run.host_tax_id · “9606”
- body site run.scientific_name · “gut metagenome”
- life stage study.description · “healthy and B6 and B12 deficient children”
Evidence examples (one per field × route)
| sample | field | value | route | conf. | source | quote |
|---|---|---|---|---|---|---|
| SAMN41489713 | age_at_collection_days | 1158.5 | R1 | 0.85 | sample.attr.age | 3.17 |
| SAMN41489713 | antibiotic_exposure | no | R1 | 0.90 | biosample_attr | No |
| SAMN41489717 | antibiotic_exposure | no | R1 | 0.85 | biosample.attribute:antibiotics_supplements | vit supplements |
| SAMN41489775 | antibiotic_exposure | yes | R1 | 0.80 | sample.attr.antibiotics_supplements | took antibiotics 2 months before |
| SAMN41489713 | bmi | 11.53 | R1 | 0.90 | sample.attr.bmi | 11.53402537 |
| SAMN41489713 | collection_date | 2023-01-21 | R1 | 0.90 | biosample.attribute:collection_date | 2023-01-21 |
| SAMN41489713 | country | IN | R1 | 0.90 | sample.attr.geo_loc_name | India: Balaghat, Madhya Pradesh |
| SAMN41489713 | diet | omnivore | R1 | 0.90 | biosample.attribute:diet | Nonvegetarian |
| SAMN41489713 | diet_detail | Nonvegetarian | R1 | 0.90 | biosample.attribute:diet | Nonvegetarian |
| SAMN41489713 | health_condition | malnutrition | R1 | 0.50 | biosample_attr | B12 deficient |
| SAMN41489713 | health_condition_detail | B12 deficient | R1 | 0.90 | sample.attr.condition | B12 deficient |
| SAMN41489713 | latitude | 21.8213 | R1 | 0.90 | biosample.attribute:lat_lon | 21.82125177 N 80.18976658 E |
| SAMN41489714 | lifestyle | vegetarian_or_vegan | R1 | 0.85 | biosample.attribute:diet | Vegetarian |
| SAMN41489714 | lifestyle_detail | Vegetarian | R1 | 0.85 | biosample.attribute:diet | Vegetarian |
| SAMN41489713 | location_locality | Balaghat | R1 | 0.85 | biosample.attribute:geo_loc_name | India: Balaghat, Madhya Pradesh |
| SAMN41489713 | location_region | Madhya Pradesh | R1 | 0.85 | biosample.attribute:geo_loc_name | India: Balaghat, Madhya Pradesh |
| SAMN41489713 | longitude | 80.1898 | R1 | 0.90 | biosample.attribute:lat_lon | 21.82125177 N 80.18976658 E |
| SAMN41489713 | sex | female | R1 | 0.90 | sample.attr.gender | female |
Sequencing
64 runs over 64 samples · mean 7.73 Gbp per run (median 7.62 Gbp, total 494.96 Gbp; 64 runs with a base count) · layout PAIRED 64 · platform ILLUMINA 64 · models Illumina NovaSeq 6000 (64) · 97% of runs with a Sandpiper profile · first public 2026-01-02
Authors (0)
No author record.
Downloads
PRJNA1114651.csv.gz (3.3 KB) · parquet (75.4 KB) — all 64 samples, every field with route and confidence · PRJNA1114651_determinations.csv.gz (7.5 KB) — 899 evidence rows