Home › Studies › PRJNA1124620
PRJNA1124620 SRP514635

Longitudinal gut microbiota in healthy adults, Maribor, Slovenia

Longitudinal study of human gut microbiota (untreated and ethanol treated paired stool samples)

201 stool samples from healthy adults in Maribor, Slovenia, collected longitudinally over 6 months (~every 14 days), studying persistence of ethanol-resistant spore-forming gut bacteria via 16S and shotgun metagenomic sequencing. machine-written summary

201 samples · 201 runs · SI · Adult · 2026 · 7.24 Gbp / sample

Sources
✓Sequence archive 3,226–Abstract / description–Full text–Supplementary tables–External resources–User contribution–Human verified

Checked this project's papers and archive records yourself? Mark it human-verified.

what each source gave
✓ Sequence archive3,226 sample values (age, bmi, collection date, country, diet, diet detail, health condition, health condition detail)
– Abstract / descriptionno abstract or project description
– Full textno open-access full text linked
– Supplementary tablesno supplementary tables available (or none open access)
– External resourcesnot covered by the external resources we ingest
– User contributionnothing uploaded yet
– Human verifiednot yet checked by a person · I checked this project — verify it
Samples at a glance
1 sampling locations · 1 country
Age
adult201
Condition
healthy_control201
Sex
male201
Country
SI201
Body site
primary201
Collected 2022–2023
2022: 157 samples2023: 44 samples20222023
Sequencing depth per sample · this project all projects
all projects, 0–0.03 Gbp: 25,080 samples (4 %)all projects, 0.03–0.1 Gbp: 17,066 samples (3 %)all projects, 0.1–0.3 Gbp: 29,179 samples (5 %)this project, 0.1–0.3 Gbp: 2 samplesall projects, 0.3–1 Gbp: 47,044 samples (8 %)this project, 0.3–1 Gbp: 21 samplesall projects, 1–3 Gbp: 124,983 samples (22 %)this project, 1–3 Gbp: 16 samplesall projects, 3–10 Gbp: 253,728 samples (44 %)this project, 3–10 Gbp: 162 samplesall projects, 10–30 Gbp: 72,533 samples (13 %)all projects, 30–100 Gbp: 3,024 samples (1 %)all projects, 100–300 Gbp: 106 samples (0 %)all projects, ≥ 300 Gbp: 9 samples (0 %)project median 7.24 Gbp per sample0130Gbp
Sampling design and relatedness

longitudinal

9 of 9 people sampled more than once (200 samples; median 22, up to 27 per person) · follow-up median 5 mo (max 6 mo) · median interval between samples 14 d (time from collection dates). Schedule stated by the source: “Stool samples collected approximately every 14 days over 6 months from the same 9 individuals”; stated follow-up 6 mo.

AA: day 0A: day 13A: day 14A: day 27A: day 43A: day 43A: day 61A: day 72A: day 72A: day 84A: day 84A: day 98A: day 98A: day 112A: day 112A: day 126A: day 126A: day 140A: day 140A: day 154A: day 154BB: day 0B: day 26B: day 43B: day 55B: day 55B: day 69B: day 69B: day 83B: day 83B: day 102B: day 111B: day 125B: day 125B: day 140B: day 153B: day 167CC: day 0C: day 0C: day 14C: day 14C: day 28C: day 28C: day 49C: day 49C: day 49C: day 57C: day 57C: day 70C: day 70C: day 84C: day 84C: day 99C: day 99C: day 112C: day 112C: day 126C: day 126C: day 140C: day 155C: day 155DD: day 0D: day 0D: day 23D: day 23D: day 43D: day 43D: day 60D: day 60D: day 69D: day 69D: day 76D: day 76D: day 92D: day 92D: day 107D: day 107D: day 121D: day 121D: day 135D: day 135D: day 149D: day 149D: day 164D: day 164D: day 184D: day 184D: day 191EE: day 0E: day 0E: day 14E: day 14E: day 28E: day 28E: day 42E: day 42E: day 61E: day 61E: day 72E: day 72E: day 85E: day 85E: day 101E: day 101E: day 108E: day 108E: day 112E: day 112E: day 119E: day 126E: day 126E: day 140E: day 140E: day 154E: day 154FF: day 0F: day 0F: day 14F: day 28F: day 28F: day 42F: day 42F: day 56F: day 56F: day 71F: day 71F: day 84F: day 84F: day 98F: day 98F: day 126F: day 126F: day 147F: day 147F: day 154F: day 168F: day 168GG: day 0G: day 14G: day 29G: day 29G: day 42G: day 42G: day 58G: day 58G: day 70G: day 70G: day 84G: day 84G: day 100G: day 100G: day 112G: day 112G: day 126G: day 126G: day 141G: day 155G: day 169G: day 169HH: day 0H: day 0H: day 15H: day 15H: day 30H: day 30H: day 57H: day 61H: day 61H: day 71H: day 75H: day 84H: day 98H: day 98H: day 113H: day 113H: day 126H: day 126H: day 142H: day 142H: day 152H: day 152II: day 0I: day 0I: day 13I: day 28I: day 28I: day 42I: day 42I: day 56I: day 70I: day 70I: day 84I: day 98I: day 98I: day 112I: day 125I: day 125I: day 140I: day 140I: day 1540 d48 d3 mo5 mo6 mo

Each row is one person (9 shown); dots are samples at days since that person's first sample.

How longitudinal and relatedness information is derived

Community composition

No Sandpiper profile for this project's samples yet.

Field coverage
fieldsamplescoverageR1R2R3R4
age_category201 100%
country201 100%201
health_condition201 100%201
subject_id200 100%200
age_at_collection_days201 100%201
bmi201 100%201
collection_date201 100%201
detailed_location201 100%
diet201 100%201
diet_detail201 100%201
health_condition_detail201 100%201
latitude201 100%201
location_locality201 100%201
location_region201 100%201
longitude201 100%201
sex201 100%201
stool_consistency_bristol201 100%201
timepoint_label200 100%200
medication7 3%7
medication_detail5 2%5
Details
Archive description

Sequences were produced by amplicon sequencing of V3-V4 region of the 16S gene and shotgun metagenomic sequencing from stool samples of 9 healthy individuals in Maribor, Slovenia, sampled for 6 months approximately 14 days. Goal of the study was to observe and describe the longitudinal persistence and stability of ethanol resistant spore-forming bacteria in the healthy human gut.

How this project entered the registry

Classified as human gut / stool mixed assays of adult by sonnet_x2 with confidence 0.57. Assay mixed.

  • host run.host_tax_id · “9606”
  • body site study.description · “stool samples of 9 healthy individuals”
  • life stage study.title · “human gut microbiota”
Evidence examples (one per field × route)
samplefieldvaluerouteconf.sourcequote
SAMN56525638age_at_collection_days12417.9R10.85sample.attr.host_age34
SAMN56525638bmi22.61R10.90sample.attr.host_body_mass_index22.605591909577633
SAMN56525638collection_date2022-07-20R10.90biosample.attribute:collection_date2022-07-20
SAMN56525638countrySIR10.90sample.attr.geo_loc_nameSlovenia: Maribor
SAMN56525638dietomnivoreR10.90biosample.attribute:host_dietomnivore
SAMN56525638diet_detailomnivoreR10.90biosample.attribute:host_dietomnivore
SAMN56525638health_conditionhealthy_controlR10.85biosample.attribute:gastrointest_disordNO
SAMN56525638health_condition_detailNOR10.90sample.attr.gastrointest_disordNO
SAMN56525638latitude46.5547R10.90biosample.attribute:lat_lon46.5547 N 15.6459 E
SAMN56525638location_localityMariborR10.85biosample.attribute:geo_loc_nameSlovenia: Maribor
SAMN56525638location_regionMariborR10.60biosample.attribute:lat_lon46.5547 N 15.6459 E
SAMN56525638longitude15.6459R10.90biosample.attribute:lat_lon46.5547 N 15.6459 E
SAMN56525652medicationother_medicationR10.85biosample.attribute:medicationantimalarik Malarone
SAMN56525692medication_detailemtricelabiniR10.90biosample.attribute:medicationemtricelabini
SAMN56525638sexmaleR10.90sample.attr.host_sexmale
SAMN56525638stool_consistency_bristol3R10.90biosample.attribute:bristol_stool_scale3
SAMN56525638subject_idAR10.70sample.name_rule:library_nameslibrary_names: mMA001
SAMN56525638timepoint_label001R10.70sample.name_rule:library_nameslibrary_names: mMA001
Sequencing

201 runs over 201 samples · mean 5.81 Gbp per run (median 7.24 Gbp, total 1,168.05 Gbp; 201 runs with a base count) · layout PAIRED 201 · platform BGISEQ 201 · models BGISEQ-500 (201) · 0% of runs with a Sandpiper profile · first public 2026-04-19

Authors (0)

No author record.

Downloads

PRJNA1124620.csv.gz (6.7 KB) · parquet (81.6 KB) — all 201 samples, every field with route and confidence · PRJNA1124620_determinations.csv.gz (24.9 KB) — 3,226 evidence rows

Samples (201 · every field this project has values for · hover a cell for its source)
Loading the sample table…